Oxidative damage and phosphatidylserine expression of red blood cells in cattle experimentally infected with Theileria sergenti
In order to elucidate the mechanism of anemia in Japanese bovine theileriosis, we investigated the oxidative alteration of red blood cells (RBCs) in cattle infected with Theileria sergenti. As an index of RBC oxidation, the levels of 2',7'-dichlorofluorescin-diacetate (DCFH) oxidation and malondiald...
| Autores principales: | , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2003
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/33098 |
| _version_ | 1855526211043721216 |
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| author | Shiono, H. Yagi, Y. Chikayama, Y. Miyazaki, S. Nakamura, I. |
| author_browse | Chikayama, Y. Miyazaki, S. Nakamura, I. Shiono, H. Yagi, Y. |
| author_facet | Shiono, H. Yagi, Y. Chikayama, Y. Miyazaki, S. Nakamura, I. |
| author_sort | Shiono, H. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | In order to elucidate the mechanism of anemia in Japanese bovine theileriosis, we investigated the oxidative alteration of red blood cells (RBCs) in cattle infected with Theileria sergenti. As an index of RBC oxidation, the levels of 2',7'-dichlorofluorescin-diacetate (DCFH) oxidation and malondialdehyde-thiobarbituric acid reactive substances (MDA-TBARS), and phosphatidylserine (PS) expression accompanying anemia were examined in experimentally infected cattle. Before the development of anemia, the concentrations of DCFH oxidation and MDA-TBARS were low, and PS expression on the surfaces of RBCs was hardly seen. However, during the onset of anemia, these levels began to increase remarkably in proportion to the decrease of packed cell volume and the increase of parasitemia in all infected cattle. During the serious stage of anemia, these oxidative indices reached their maximum values. Our findings indicate that oxidative damage and loss of membrane asymmetry in RBCs are related to the development of anemia in T. sergenti infection. This oxidative damage to the RBCs might play an important role in the pathogenesis of anemia in Japanese bovine theileriosis. |
| format | Journal Article |
| id | CGSpace33098 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2003 |
| publishDateRange | 2003 |
| publishDateSort | 2003 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace330982023-12-08T19:36:04Z Oxidative damage and phosphatidylserine expression of red blood cells in cattle experimentally infected with Theileria sergenti Shiono, H. Yagi, Y. Chikayama, Y. Miyazaki, S. Nakamura, I. cattle theileria sergenti oxidative stress anaemia erythrocytes experimental infections oxidation peroxides phosphatidylserines theileriosis In order to elucidate the mechanism of anemia in Japanese bovine theileriosis, we investigated the oxidative alteration of red blood cells (RBCs) in cattle infected with Theileria sergenti. As an index of RBC oxidation, the levels of 2',7'-dichlorofluorescin-diacetate (DCFH) oxidation and malondialdehyde-thiobarbituric acid reactive substances (MDA-TBARS), and phosphatidylserine (PS) expression accompanying anemia were examined in experimentally infected cattle. Before the development of anemia, the concentrations of DCFH oxidation and MDA-TBARS were low, and PS expression on the surfaces of RBCs was hardly seen. However, during the onset of anemia, these levels began to increase remarkably in proportion to the decrease of packed cell volume and the increase of parasitemia in all infected cattle. During the serious stage of anemia, these oxidative indices reached their maximum values. Our findings indicate that oxidative damage and loss of membrane asymmetry in RBCs are related to the development of anemia in T. sergenti infection. This oxidative damage to the RBCs might play an important role in the pathogenesis of anemia in Japanese bovine theileriosis. 2003-02 2013-07-03T05:26:03Z 2013-07-03T05:26:03Z Journal Article https://hdl.handle.net/10568/33098 en Limited Access Springer Parasitology Research;89(3): 228-234 |
| spellingShingle | cattle theileria sergenti oxidative stress anaemia erythrocytes experimental infections oxidation peroxides phosphatidylserines theileriosis Shiono, H. Yagi, Y. Chikayama, Y. Miyazaki, S. Nakamura, I. Oxidative damage and phosphatidylserine expression of red blood cells in cattle experimentally infected with Theileria sergenti |
| title | Oxidative damage and phosphatidylserine expression of red blood cells in cattle experimentally infected with Theileria sergenti |
| title_full | Oxidative damage and phosphatidylserine expression of red blood cells in cattle experimentally infected with Theileria sergenti |
| title_fullStr | Oxidative damage and phosphatidylserine expression of red blood cells in cattle experimentally infected with Theileria sergenti |
| title_full_unstemmed | Oxidative damage and phosphatidylserine expression of red blood cells in cattle experimentally infected with Theileria sergenti |
| title_short | Oxidative damage and phosphatidylserine expression of red blood cells in cattle experimentally infected with Theileria sergenti |
| title_sort | oxidative damage and phosphatidylserine expression of red blood cells in cattle experimentally infected with theileria sergenti |
| topic | cattle theileria sergenti oxidative stress anaemia erythrocytes experimental infections oxidation peroxides phosphatidylserines theileriosis |
| url | https://hdl.handle.net/10568/33098 |
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