Genetic and phenotypic analysis of Tunisian Theileria annulata clones
Many parasite species are known to show high levels of genetic diversity, yet the consequences of this diversity for host-parasite interactions are not well understood. Variation in phenotypic traits such as growth rates and the ability to form transmission stages are raw material for natural and ar...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Cambridge University Press
2003
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/33096 |
| _version_ | 1855521951830769664 |
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| author | Taylor, L.H. Katzer, F. Shiels, B.R. Welburn, S.C. |
| author_browse | Katzer, F. Shiels, B.R. Taylor, L.H. Welburn, S.C. |
| author_facet | Taylor, L.H. Katzer, F. Shiels, B.R. Welburn, S.C. |
| author_sort | Taylor, L.H. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Many parasite species are known to show high levels of genetic diversity, yet the consequences of this diversity for host-parasite interactions are not well understood. Variation in phenotypic traits such as growth rates and the ability to form transmission stages are raw material for natural and artificial selection to act upon with consequences for the evolution of the parasite species and disease control. In order to study genetic and phenotypic diversity amongst Theileria annulata parasites, a collection of 52 parasite clones was generated from cattle isolates and tick material recently collected in Tunisia. Genetic diversity was assessed using PCR-RFLP and monoclonal antibody markers, and genetically distinct clones selected for further study. Clones varied significantly in their growth rates in culture at 37 degreesC, their viability after a period of culture at 41 degreesC and their differentiation rates into transmission stages after culturing at 41 degreesC. The viability of a clone after culturing at 41 degreesC could not be predicted from its growth rate at 37 degreesC, but across clones, differentiation rates were positively correlated with growth rates at 37 degreesC. All 3 in vitro measures are likely to have relevance to parasite-host interactions in animals with clinical theileriosis, and should be acted on by within-host and between-host selection. |
| format | Journal Article |
| id | CGSpace33096 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2003 |
| publishDateRange | 2003 |
| publishDateSort | 2003 |
| publisher | Cambridge University Press |
| publisherStr | Cambridge University Press |
| record_format | dspace |
| spelling | CGSpace330962024-11-15T08:52:22Z Genetic and phenotypic analysis of Tunisian Theileria annulata clones Taylor, L.H. Katzer, F. Shiels, B.R. Welburn, S.C. tunisia cattle theileria annulata clones genetic variation genotypes growth rate molecular genetics phenotypes Many parasite species are known to show high levels of genetic diversity, yet the consequences of this diversity for host-parasite interactions are not well understood. Variation in phenotypic traits such as growth rates and the ability to form transmission stages are raw material for natural and artificial selection to act upon with consequences for the evolution of the parasite species and disease control. In order to study genetic and phenotypic diversity amongst Theileria annulata parasites, a collection of 52 parasite clones was generated from cattle isolates and tick material recently collected in Tunisia. Genetic diversity was assessed using PCR-RFLP and monoclonal antibody markers, and genetically distinct clones selected for further study. Clones varied significantly in their growth rates in culture at 37 degreesC, their viability after a period of culture at 41 degreesC and their differentiation rates into transmission stages after culturing at 41 degreesC. The viability of a clone after culturing at 41 degreesC could not be predicted from its growth rate at 37 degreesC, but across clones, differentiation rates were positively correlated with growth rates at 37 degreesC. All 3 in vitro measures are likely to have relevance to parasite-host interactions in animals with clinical theileriosis, and should be acted on by within-host and between-host selection. 2003-03 2013-07-03T05:26:03Z 2013-07-03T05:26:03Z Journal Article https://hdl.handle.net/10568/33096 en Limited Access Cambridge University Press Parasitology;126(3): 241-252 |
| spellingShingle | tunisia cattle theileria annulata clones genetic variation genotypes growth rate molecular genetics phenotypes Taylor, L.H. Katzer, F. Shiels, B.R. Welburn, S.C. Genetic and phenotypic analysis of Tunisian Theileria annulata clones |
| title | Genetic and phenotypic analysis of Tunisian Theileria annulata clones |
| title_full | Genetic and phenotypic analysis of Tunisian Theileria annulata clones |
| title_fullStr | Genetic and phenotypic analysis of Tunisian Theileria annulata clones |
| title_full_unstemmed | Genetic and phenotypic analysis of Tunisian Theileria annulata clones |
| title_short | Genetic and phenotypic analysis of Tunisian Theileria annulata clones |
| title_sort | genetic and phenotypic analysis of tunisian theileria annulata clones |
| topic | tunisia cattle theileria annulata clones genetic variation genotypes growth rate molecular genetics phenotypes |
| url | https://hdl.handle.net/10568/33096 |
| work_keys_str_mv | AT taylorlh geneticandphenotypicanalysisoftunisiantheileriaannulataclones AT katzerf geneticandphenotypicanalysisoftunisiantheileriaannulataclones AT shielsbr geneticandphenotypicanalysisoftunisiantheileriaannulataclones AT welburnsc geneticandphenotypicanalysisoftunisiantheileriaannulataclones |