Analysis of cellular responses to native and recombinant proteins of Cowdria ruminantium
The response of peripheral blood mononuclear cells (PBMC) collected from cattle immunized with killed C. ruminantium to MAP1, MAP1-B, MAP2 and to a lysate of Cowdria was characterized in vitro. CD4+ T lymphocytes responded strongly to Cowdria lysates while B cells and gamma delta T cells proliferate...
| Autores principales: | , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
1998
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/33077 |
| _version_ | 1855535311747022848 |
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| author | Totte, P. McKeever, Declan J. Jongejan, F. Barbet, A. Mahan, S.M. Mwangi, D. Bensaid, A. |
| author_browse | Barbet, A. Bensaid, A. Jongejan, F. Mahan, S.M. McKeever, Declan J. Mwangi, D. Totte, P. |
| author_facet | Totte, P. McKeever, Declan J. Jongejan, F. Barbet, A. Mahan, S.M. Mwangi, D. Bensaid, A. |
| author_sort | Totte, P. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The response of peripheral blood mononuclear cells (PBMC) collected from cattle immunized with killed C. ruminantium to MAP1, MAP1-B, MAP2 and to a lysate of Cowdria was characterized in vitro. CD4+ T lymphocytes responded strongly to Cowdria lysates while B cells and gamma delta T cells proliferated in response to recombinant proteins. Short-term cell lines derived from PBMC using Cowdria lysates were >90% CD4+ and did not respond to any of the recombinant proteins. Restimulation of PBMC with MAP1, MAP1-B, and MAP2 proved difficult, and cultures usually stopped proliferating after 2 to 3 weeks. Only 1 cell line could be generated using MAP1 and it was composed of 85% gamma delta T cells. This study indicates that in animals immunized with killed Cowdria, proteins other than the serologically immunodominant MAP1 and MAP2 are strongly immunogenic for T lymphocytes. |
| format | Journal Article |
| id | CGSpace33077 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1998 |
| publishDateRange | 1998 |
| publishDateSort | 1998 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace330772024-05-01T08:18:56Z Analysis of cellular responses to native and recombinant proteins of Cowdria ruminantium Totte, P. McKeever, Declan J. Jongejan, F. Barbet, A. Mahan, S.M. Mwangi, D. Bensaid, A. cattle ruminants cowdria ruminantium proteins recombinant proteins lymphocytes antigens immune response rickettsiales bacterial diseases The response of peripheral blood mononuclear cells (PBMC) collected from cattle immunized with killed C. ruminantium to MAP1, MAP1-B, MAP2 and to a lysate of Cowdria was characterized in vitro. CD4+ T lymphocytes responded strongly to Cowdria lysates while B cells and gamma delta T cells proliferated in response to recombinant proteins. Short-term cell lines derived from PBMC using Cowdria lysates were >90% CD4+ and did not respond to any of the recombinant proteins. Restimulation of PBMC with MAP1, MAP1-B, and MAP2 proved difficult, and cultures usually stopped proliferating after 2 to 3 weeks. Only 1 cell line could be generated using MAP1 and it was composed of 85% gamma delta T cells. This study indicates that in animals immunized with killed Cowdria, proteins other than the serologically immunodominant MAP1 and MAP2 are strongly immunogenic for T lymphocytes. 1998-06 2013-07-03T05:26:01Z 2013-07-03T05:26:01Z Journal Article https://hdl.handle.net/10568/33077 en Limited Access Wiley New York Academy of Sciences. Annals;849: 155-160 |
| spellingShingle | cattle ruminants cowdria ruminantium proteins recombinant proteins lymphocytes antigens immune response rickettsiales bacterial diseases Totte, P. McKeever, Declan J. Jongejan, F. Barbet, A. Mahan, S.M. Mwangi, D. Bensaid, A. Analysis of cellular responses to native and recombinant proteins of Cowdria ruminantium |
| title | Analysis of cellular responses to native and recombinant proteins of Cowdria ruminantium |
| title_full | Analysis of cellular responses to native and recombinant proteins of Cowdria ruminantium |
| title_fullStr | Analysis of cellular responses to native and recombinant proteins of Cowdria ruminantium |
| title_full_unstemmed | Analysis of cellular responses to native and recombinant proteins of Cowdria ruminantium |
| title_short | Analysis of cellular responses to native and recombinant proteins of Cowdria ruminantium |
| title_sort | analysis of cellular responses to native and recombinant proteins of cowdria ruminantium |
| topic | cattle ruminants cowdria ruminantium proteins recombinant proteins lymphocytes antigens immune response rickettsiales bacterial diseases |
| url | https://hdl.handle.net/10568/33077 |
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