In vivo inhibition of trypanosome mitochondrial topoisomerase II: Effects of kinetoplast DNA maxicircles
Kinetoplast DNA, the mitochondrial DNA of trypanosomes, is a topologically complex structure composed of interlocked minicircles and maxicircles. We previously reported that etoposide, a potent inhibitor of topoisomerase II, promotes the cleavage of about 20% of network minicircle DNA (T. A. Shapiro...
| Main Authors: | , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Informa UK Limited
1994
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/33069 |
| _version_ | 1855514088160886784 |
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| author | Shapiro, T.A. Showalter, F. |
| author_browse | Shapiro, T.A. Showalter, F. |
| author_facet | Shapiro, T.A. Showalter, F. |
| author_sort | Shapiro, T.A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Kinetoplast DNA, the mitochondrial DNA of trypanosomes, is a topologically complex structure composed of interlocked minicircles and maxicircles. We previously reported that etoposide, a potent inhibitor of topoisomerase II, promotes the cleavage of about 20% of network minicircle DNA (T. A. Shapiro, V. A. Klein, and P. T. Englund, J. Biol. Chem. 264:4173-4178, 1989). We now find that virtually all maxicircles are released from kinetoplast DNA networks after trypanosomes are treated with etoposide. As expected for a topoisomerase II cleavage product, the linearized maxicircles have protein bound to both 5' ends. After etoposide treatment, the residual minicircle catenanes have a sedimentation coefficient which is only 70% that of controls, and by electron microscopy the networks are less compact. Double-size networks, the characteristic dumbbell-shape forms that normally arise in the final stages of network replication, are replaced by aberrant unit-size forms. |
| format | Journal Article |
| id | CGSpace33069 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1994 |
| publishDateRange | 1994 |
| publishDateSort | 1994 |
| publisher | Informa UK Limited |
| publisherStr | Informa UK Limited |
| record_format | dspace |
| spelling | CGSpace330692024-05-01T08:15:18Z In vivo inhibition of trypanosome mitochondrial topoisomerase II: Effects of kinetoplast DNA maxicircles Shapiro, T.A. Showalter, F. trypanosome dna mitochondria animal diseases cell biology molecular biology Kinetoplast DNA, the mitochondrial DNA of trypanosomes, is a topologically complex structure composed of interlocked minicircles and maxicircles. We previously reported that etoposide, a potent inhibitor of topoisomerase II, promotes the cleavage of about 20% of network minicircle DNA (T. A. Shapiro, V. A. Klein, and P. T. Englund, J. Biol. Chem. 264:4173-4178, 1989). We now find that virtually all maxicircles are released from kinetoplast DNA networks after trypanosomes are treated with etoposide. As expected for a topoisomerase II cleavage product, the linearized maxicircles have protein bound to both 5' ends. After etoposide treatment, the residual minicircle catenanes have a sedimentation coefficient which is only 70% that of controls, and by electron microscopy the networks are less compact. Double-size networks, the characteristic dumbbell-shape forms that normally arise in the final stages of network replication, are replaced by aberrant unit-size forms. 1994-09 2013-07-03T05:26:01Z 2013-07-03T05:26:01Z Journal Article https://hdl.handle.net/10568/33069 en Limited Access Informa UK Limited Molecular and Cellular Biology;14: 5891-5897 |
| spellingShingle | trypanosome dna mitochondria animal diseases cell biology molecular biology Shapiro, T.A. Showalter, F. In vivo inhibition of trypanosome mitochondrial topoisomerase II: Effects of kinetoplast DNA maxicircles |
| title | In vivo inhibition of trypanosome mitochondrial topoisomerase II: Effects of kinetoplast DNA maxicircles |
| title_full | In vivo inhibition of trypanosome mitochondrial topoisomerase II: Effects of kinetoplast DNA maxicircles |
| title_fullStr | In vivo inhibition of trypanosome mitochondrial topoisomerase II: Effects of kinetoplast DNA maxicircles |
| title_full_unstemmed | In vivo inhibition of trypanosome mitochondrial topoisomerase II: Effects of kinetoplast DNA maxicircles |
| title_short | In vivo inhibition of trypanosome mitochondrial topoisomerase II: Effects of kinetoplast DNA maxicircles |
| title_sort | in vivo inhibition of trypanosome mitochondrial topoisomerase ii effects of kinetoplast dna maxicircles |
| topic | trypanosome dna mitochondria animal diseases cell biology molecular biology |
| url | https://hdl.handle.net/10568/33069 |
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