Putative genes of a variant-specific antigen gene transcription unit in Trypanosoma brucei
In a 7-kilobase (kb) sequence upstream from the 5' barren region, the Trypanosoma brucei AnTat 1.3A expression site carries two putative genes, named ESAG 2 and ESAG 3 for expression site-associated genes, as well as a copy of ESAG 1 (D.F. Cully, H.S. Ip, and G.A.M. Cross, Cell 42:173-182, 1985). At...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Informa UK Limited
1988
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/33068 |
| _version_ | 1855515180780224512 |
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| author | Alexandre, S. Guyaux, M. Murphy, N.B. Coquelet, H. Pays, A. Steinert, M. Pays, E. |
| author_browse | Alexandre, S. Coquelet, H. Guyaux, M. Murphy, N.B. Pays, A. Pays, E. Steinert, M. |
| author_facet | Alexandre, S. Guyaux, M. Murphy, N.B. Coquelet, H. Pays, A. Steinert, M. Pays, E. |
| author_sort | Alexandre, S. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | In a 7-kilobase (kb) sequence upstream from the 5' barren region, the Trypanosoma brucei AnTat 1.3A expression site carries two putative genes, named ESAG 2 and ESAG 3 for expression site-associated genes, as well as a copy of ESAG 1 (D.F. Cully, H.S. Ip, and G.A.M. Cross, Cell 42:173-182, 1985). At least 3 kb of this expression site exhibits a high degree of homology with the silent telomere carrying the AnTat 1.3A basic copy, whose ESAG 1 is interrupted by stop codons. Like the antigen gene, the region containing the ESAGs is transcribed only in the bloodstream forms, although transcription of 5' barren- and ESAG 2-related sequences also occurs in cultured procyclics. Analysis of steady-state and nascent transcripts suggests a continuous transcription of the whole expression site by an RNA polymerase resistant to alpha-amanitin, possibly initiating at a polymerase I-like promoter located about 17 kb upstream from the antigen gene. This polymerase seems prone to becoming inactivated upon incubation of the trypanosomes at low temperature. The putative protein encoded by ESAG 3 may carry a hydrophobic signal peptide, suggesting interaction with a membrane. |
| format | Journal Article |
| id | CGSpace33068 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1988 |
| publishDateRange | 1988 |
| publishDateSort | 1988 |
| publisher | Informa UK Limited |
| publisherStr | Informa UK Limited |
| record_format | dspace |
| spelling | CGSpace330682024-05-01T08:15:21Z Putative genes of a variant-specific antigen gene transcription unit in Trypanosoma brucei Alexandre, S. Guyaux, M. Murphy, N.B. Coquelet, H. Pays, A. Steinert, M. Pays, E. trypanosoma brucei antigens genes cell biology molecular biology In a 7-kilobase (kb) sequence upstream from the 5' barren region, the Trypanosoma brucei AnTat 1.3A expression site carries two putative genes, named ESAG 2 and ESAG 3 for expression site-associated genes, as well as a copy of ESAG 1 (D.F. Cully, H.S. Ip, and G.A.M. Cross, Cell 42:173-182, 1985). At least 3 kb of this expression site exhibits a high degree of homology with the silent telomere carrying the AnTat 1.3A basic copy, whose ESAG 1 is interrupted by stop codons. Like the antigen gene, the region containing the ESAGs is transcribed only in the bloodstream forms, although transcription of 5' barren- and ESAG 2-related sequences also occurs in cultured procyclics. Analysis of steady-state and nascent transcripts suggests a continuous transcription of the whole expression site by an RNA polymerase resistant to alpha-amanitin, possibly initiating at a polymerase I-like promoter located about 17 kb upstream from the antigen gene. This polymerase seems prone to becoming inactivated upon incubation of the trypanosomes at low temperature. The putative protein encoded by ESAG 3 may carry a hydrophobic signal peptide, suggesting interaction with a membrane. 1988-06 2013-07-03T05:26:01Z 2013-07-03T05:26:01Z Journal Article https://hdl.handle.net/10568/33068 en Limited Access Informa UK Limited Molecular and Cellular Biology;8: 2367-2378 |
| spellingShingle | trypanosoma brucei antigens genes cell biology molecular biology Alexandre, S. Guyaux, M. Murphy, N.B. Coquelet, H. Pays, A. Steinert, M. Pays, E. Putative genes of a variant-specific antigen gene transcription unit in Trypanosoma brucei |
| title | Putative genes of a variant-specific antigen gene transcription unit in Trypanosoma brucei |
| title_full | Putative genes of a variant-specific antigen gene transcription unit in Trypanosoma brucei |
| title_fullStr | Putative genes of a variant-specific antigen gene transcription unit in Trypanosoma brucei |
| title_full_unstemmed | Putative genes of a variant-specific antigen gene transcription unit in Trypanosoma brucei |
| title_short | Putative genes of a variant-specific antigen gene transcription unit in Trypanosoma brucei |
| title_sort | putative genes of a variant specific antigen gene transcription unit in trypanosoma brucei |
| topic | trypanosoma brucei antigens genes cell biology molecular biology |
| url | https://hdl.handle.net/10568/33068 |
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