Acetyldiaminobutanoic acid, a potential lathyrogenic amino acid in leaves of Acacia angustissima
Acacia angustissima has potential in agroforestry systems for forage production in tropical environments. However, feeding trials indicate that the leaves are potentially toxic. Signs of toxicity in sheep are similar to those presented by sheep fed flatpea (Lathyrus sylvestris) hay which contains a...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Wiley
2001
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/33022 |
| _version_ | 1855540995001679872 |
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| author | Reed, J.D. Gebre-Mariam, G. Robinson, C.J. Hanson, Jean Odenyo, A.A. Treichel, P.M. |
| author_browse | Gebre-Mariam, G. Hanson, Jean Odenyo, A.A. Reed, J.D. Robinson, C.J. Treichel, P.M. |
| author_facet | Reed, J.D. Gebre-Mariam, G. Robinson, C.J. Hanson, Jean Odenyo, A.A. Treichel, P.M. |
| author_sort | Reed, J.D. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Acacia angustissima has potential in agroforestry systems for forage production in tropical environments. However, feeding trials indicate that the leaves are potentially toxic. Signs of toxicity in sheep are similar to those presented by sheep fed flatpea (Lathyrus sylvestris) hay which contains a high concentration of the non-protein amino acid 2,4-diaminobutanoic acid (DABA). Cation exchange chromatography, high-performance liquid chromatography (HPLC) and NMR were used to isolate and identify the non-protein amino acid in A angustissima leaves. A quantitative HPLC method was used to determine the content of the non-protein amino acid in leaves from 20 accessions. The non-protein amino acid was identified as 4-N-acetyl-2,4-diaminobutanoic acid (ADAB), previously reported to occur in seeds. The average and range in ADAB content were 16.8 and 10.6-25.4mgg -1 respectively. This content of ADAB is higher than the levels of DABA in flatpea hay that are toxic to sheep. |
| format | Journal Article |
| id | CGSpace33022 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2001 |
| publishDateRange | 2001 |
| publishDateSort | 2001 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace330222023-07-03T16:47:37Z Acetyldiaminobutanoic acid, a potential lathyrogenic amino acid in leaves of Acacia angustissima Reed, J.D. Gebre-Mariam, G. Robinson, C.J. Hanson, Jean Odenyo, A.A. Treichel, P.M. acacia angustissima amino acids leaves hplc poisoning cations ions nmr spectroscopy chemical composition analytical methods Acacia angustissima has potential in agroforestry systems for forage production in tropical environments. However, feeding trials indicate that the leaves are potentially toxic. Signs of toxicity in sheep are similar to those presented by sheep fed flatpea (Lathyrus sylvestris) hay which contains a high concentration of the non-protein amino acid 2,4-diaminobutanoic acid (DABA). Cation exchange chromatography, high-performance liquid chromatography (HPLC) and NMR were used to isolate and identify the non-protein amino acid in A angustissima leaves. A quantitative HPLC method was used to determine the content of the non-protein amino acid in leaves from 20 accessions. The non-protein amino acid was identified as 4-N-acetyl-2,4-diaminobutanoic acid (ADAB), previously reported to occur in seeds. The average and range in ADAB content were 16.8 and 10.6-25.4mgg -1 respectively. This content of ADAB is higher than the levels of DABA in flatpea hay that are toxic to sheep. 2001-12 2013-07-03T05:25:56Z 2013-07-03T05:25:56Z Journal Article https://hdl.handle.net/10568/33022 en Limited Access Wiley Journal of the Science of Food and Agriculture;81(15): 1481-1486 |
| spellingShingle | acacia angustissima amino acids leaves hplc poisoning cations ions nmr spectroscopy chemical composition analytical methods Reed, J.D. Gebre-Mariam, G. Robinson, C.J. Hanson, Jean Odenyo, A.A. Treichel, P.M. Acetyldiaminobutanoic acid, a potential lathyrogenic amino acid in leaves of Acacia angustissima |
| title | Acetyldiaminobutanoic acid, a potential lathyrogenic amino acid in leaves of Acacia angustissima |
| title_full | Acetyldiaminobutanoic acid, a potential lathyrogenic amino acid in leaves of Acacia angustissima |
| title_fullStr | Acetyldiaminobutanoic acid, a potential lathyrogenic amino acid in leaves of Acacia angustissima |
| title_full_unstemmed | Acetyldiaminobutanoic acid, a potential lathyrogenic amino acid in leaves of Acacia angustissima |
| title_short | Acetyldiaminobutanoic acid, a potential lathyrogenic amino acid in leaves of Acacia angustissima |
| title_sort | acetyldiaminobutanoic acid a potential lathyrogenic amino acid in leaves of acacia angustissima |
| topic | acacia angustissima amino acids leaves hplc poisoning cations ions nmr spectroscopy chemical composition analytical methods |
| url | https://hdl.handle.net/10568/33022 |
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