The use of HPLC-derived phenolic profiles as a means of classifying Sesbania accessions
Aqueous acetone extracts of mature leaves from 10 accessions of two species of Sesbania plants were prepared and anlysed by high-performance liquid chromatography (HPLC). The HPLC phenolic profiles were used to develop a classification rule to distinguish between accessions. Out of the 44 mature lea...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Wiley
1996
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/33019 |
| _version_ | 1855531633514381312 |
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| author | Plumb, V.E. Wood, C.D. Gay, C. Hanson, Jean Heering, J.H. |
| author_browse | Gay, C. Hanson, Jean Heering, J.H. Plumb, V.E. Wood, C.D. |
| author_facet | Plumb, V.E. Wood, C.D. Gay, C. Hanson, Jean Heering, J.H. |
| author_sort | Plumb, V.E. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Aqueous acetone extracts of mature leaves from 10 accessions of two species of Sesbania plants were prepared and anlysed by high-performance liquid chromatography (HPLC). The HPLC phenolic profiles were used to develop a classification rule to distinguish between accessions. Out of the 44 mature leaf samples analysed, 27 had distinctive HPLC phenolic profiles and were successfully assigned to the correct accession. Seedlings of these accessions could be classified to the correct species after 3 months' growth. After 3 months' growth, 8 out of 15 samples could be assigned to the correct accession and, at 6 months, 7 out of 20. However, when accessions with very similar phenolic profiles were grouped together, there was a 100 percent success rate for classifying the correct accession at 3 months and 80 percent success rate at 6 months. It was concluded that the method could distinguish between some accessions, or groups of accessions, of Sesbania after 3 months' growth. HPLC phenolic profiling is potentially a relatively rapid, but not uniformly reliable, method for classifying Sesbania accessions. |
| format | Journal Article |
| id | CGSpace33019 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1996 |
| publishDateRange | 1996 |
| publishDateSort | 1996 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace330192024-04-25T06:01:20Z The use of HPLC-derived phenolic profiles as a means of classifying Sesbania accessions Plumb, V.E. Wood, C.D. Gay, C. Hanson, Jean Heering, J.H. sesbania sesbania goetzei sesbania sesban leaves chemical composition phenolic compounds Aqueous acetone extracts of mature leaves from 10 accessions of two species of Sesbania plants were prepared and anlysed by high-performance liquid chromatography (HPLC). The HPLC phenolic profiles were used to develop a classification rule to distinguish between accessions. Out of the 44 mature leaf samples analysed, 27 had distinctive HPLC phenolic profiles and were successfully assigned to the correct accession. Seedlings of these accessions could be classified to the correct species after 3 months' growth. After 3 months' growth, 8 out of 15 samples could be assigned to the correct accession and, at 6 months, 7 out of 20. However, when accessions with very similar phenolic profiles were grouped together, there was a 100 percent success rate for classifying the correct accession at 3 months and 80 percent success rate at 6 months. It was concluded that the method could distinguish between some accessions, or groups of accessions, of Sesbania after 3 months' growth. HPLC phenolic profiling is potentially a relatively rapid, but not uniformly reliable, method for classifying Sesbania accessions. 1996 2013-07-03T05:25:56Z 2013-07-03T05:25:56Z Journal Article https://hdl.handle.net/10568/33019 en Limited Access Wiley Journal of the Science of Food and Agriculture;70(4): 481-486 |
| spellingShingle | sesbania sesbania goetzei sesbania sesban leaves chemical composition phenolic compounds Plumb, V.E. Wood, C.D. Gay, C. Hanson, Jean Heering, J.H. The use of HPLC-derived phenolic profiles as a means of classifying Sesbania accessions |
| title | The use of HPLC-derived phenolic profiles as a means of classifying Sesbania accessions |
| title_full | The use of HPLC-derived phenolic profiles as a means of classifying Sesbania accessions |
| title_fullStr | The use of HPLC-derived phenolic profiles as a means of classifying Sesbania accessions |
| title_full_unstemmed | The use of HPLC-derived phenolic profiles as a means of classifying Sesbania accessions |
| title_short | The use of HPLC-derived phenolic profiles as a means of classifying Sesbania accessions |
| title_sort | use of hplc derived phenolic profiles as a means of classifying sesbania accessions |
| topic | sesbania sesbania goetzei sesbania sesban leaves chemical composition phenolic compounds |
| url | https://hdl.handle.net/10568/33019 |
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