Recombinant RoTat 1.2 variable surface glycoprotein as antigen for diagnosis of Trypanosoma evansi in dromedary camels
The transcript encoding a predominant Trypanosoma evansi variable surface glycoprotein RoTat 1.2 was cloned and expressed as a recombinant protein in Spodoptera frugiperda and Trichoplusia ni (insect) cells. Its potential as an antigen for specific detection of antibody in serum of dromedary camels...
| Autores principales: | , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2005
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/32959 |
| _version_ | 1855523457485242368 |
|---|---|
| author | Lejon, Veerle Claes, F. Verloo, D. Maina, M. Urakawa, T. Majiwa, Phelix A.O. Büscher, Philippe |
| author_browse | Büscher, Philippe Claes, F. Lejon, Veerle Maina, M. Majiwa, Phelix A.O. Urakawa, T. Verloo, D. |
| author_facet | Lejon, Veerle Claes, F. Verloo, D. Maina, M. Urakawa, T. Majiwa, Phelix A.O. Büscher, Philippe |
| author_sort | Lejon, Veerle |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The transcript encoding a predominant Trypanosoma evansi variable surface glycoprotein RoTat 1.2 was cloned and expressed as a recombinant protein in Spodoptera frugiperda and Trichoplusia ni (insect) cells. Its potential as an antigen for specific detection of antibody in serum of dromedary camels affected by surra, was evaluated. In ELISA, the reactivity of the recombinant RoTat 1.2 VSG was similar to that of native RoTat 1.2 VSG. An indirect agglutination reagent was therefore prepared by coupling the recombinant RoTat 1.2 VSG onto latex particles. The performance of the latex agglutination test was evaluated on camel sera, and compared with the performance of CATT/T. evansi and LATEX/T. evansi tests, using the immune trypanolysis assay with T. evansi RoTat 1.2 as a reference test. The relative sensitivity and specificity of the latex coated with recombinant RoTat 1.2 VSG, using a 1:4 serum dilution, were respectively, 89.3 and 99.1%. No differences were observed between the performance of latex coated with recombinant RoTat 1.2 VSG and LATEX/T. evansi or CATT/T. evansi. Here, we describe the successful use of the recombinant RoTat 1.2 VSG for detection of specific antibodies induced by T. evansi infections. |
| format | Journal Article |
| id | CGSpace32959 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2005 |
| publishDateRange | 2005 |
| publishDateSort | 2005 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace329592024-03-06T10:16:43Z Recombinant RoTat 1.2 variable surface glycoprotein as antigen for diagnosis of Trypanosoma evansi in dromedary camels Lejon, Veerle Claes, F. Verloo, D. Maina, M. Urakawa, T. Majiwa, Phelix A.O. Büscher, Philippe dromedaries camels [camelidae] trypanosoma evansi diagnosis glycoproteins agglutination tests latex antibodies protozoal infections recombinant proteins spodoptera frugiperda trichoplusia ni elisa The transcript encoding a predominant Trypanosoma evansi variable surface glycoprotein RoTat 1.2 was cloned and expressed as a recombinant protein in Spodoptera frugiperda and Trichoplusia ni (insect) cells. Its potential as an antigen for specific detection of antibody in serum of dromedary camels affected by surra, was evaluated. In ELISA, the reactivity of the recombinant RoTat 1.2 VSG was similar to that of native RoTat 1.2 VSG. An indirect agglutination reagent was therefore prepared by coupling the recombinant RoTat 1.2 VSG onto latex particles. The performance of the latex agglutination test was evaluated on camel sera, and compared with the performance of CATT/T. evansi and LATEX/T. evansi tests, using the immune trypanolysis assay with T. evansi RoTat 1.2 as a reference test. The relative sensitivity and specificity of the latex coated with recombinant RoTat 1.2 VSG, using a 1:4 serum dilution, were respectively, 89.3 and 99.1%. No differences were observed between the performance of latex coated with recombinant RoTat 1.2 VSG and LATEX/T. evansi or CATT/T. evansi. Here, we describe the successful use of the recombinant RoTat 1.2 VSG for detection of specific antibodies induced by T. evansi infections. 2005-04 2013-07-03T05:25:50Z 2013-07-03T05:25:50Z Journal Article https://hdl.handle.net/10568/32959 en Limited Access Elsevier International Journal for Parasitology;35(4): 455-460 |
| spellingShingle | dromedaries camels [camelidae] trypanosoma evansi diagnosis glycoproteins agglutination tests latex antibodies protozoal infections recombinant proteins spodoptera frugiperda trichoplusia ni elisa Lejon, Veerle Claes, F. Verloo, D. Maina, M. Urakawa, T. Majiwa, Phelix A.O. Büscher, Philippe Recombinant RoTat 1.2 variable surface glycoprotein as antigen for diagnosis of Trypanosoma evansi in dromedary camels |
| title | Recombinant RoTat 1.2 variable surface glycoprotein as antigen for diagnosis of Trypanosoma evansi in dromedary camels |
| title_full | Recombinant RoTat 1.2 variable surface glycoprotein as antigen for diagnosis of Trypanosoma evansi in dromedary camels |
| title_fullStr | Recombinant RoTat 1.2 variable surface glycoprotein as antigen for diagnosis of Trypanosoma evansi in dromedary camels |
| title_full_unstemmed | Recombinant RoTat 1.2 variable surface glycoprotein as antigen for diagnosis of Trypanosoma evansi in dromedary camels |
| title_short | Recombinant RoTat 1.2 variable surface glycoprotein as antigen for diagnosis of Trypanosoma evansi in dromedary camels |
| title_sort | recombinant rotat 1 2 variable surface glycoprotein as antigen for diagnosis of trypanosoma evansi in dromedary camels |
| topic | dromedaries camels [camelidae] trypanosoma evansi diagnosis glycoproteins agglutination tests latex antibodies protozoal infections recombinant proteins spodoptera frugiperda trichoplusia ni elisa |
| url | https://hdl.handle.net/10568/32959 |
| work_keys_str_mv | AT lejonveerle recombinantrotat12variablesurfaceglycoproteinasantigenfordiagnosisoftrypanosomaevansiindromedarycamels AT claesf recombinantrotat12variablesurfaceglycoproteinasantigenfordiagnosisoftrypanosomaevansiindromedarycamels AT verlood recombinantrotat12variablesurfaceglycoproteinasantigenfordiagnosisoftrypanosomaevansiindromedarycamels AT mainam recombinantrotat12variablesurfaceglycoproteinasantigenfordiagnosisoftrypanosomaevansiindromedarycamels AT urakawat recombinantrotat12variablesurfaceglycoproteinasantigenfordiagnosisoftrypanosomaevansiindromedarycamels AT majiwaphelixao recombinantrotat12variablesurfaceglycoproteinasantigenfordiagnosisoftrypanosomaevansiindromedarycamels AT buscherphilippe recombinantrotat12variablesurfaceglycoproteinasantigenfordiagnosisoftrypanosomaevansiindromedarycamels |