B-1-like cells exist in sheep. Characterization of their phenotype and behaviour
Two populations of B lymphocytes. B-1 (CD5- and or CD1 lb-) and B-2 (CD5- and CD1 lb-) cells have been described. In mice, which is the species of reference for B-1 and B-2 cell studies, these two subsets present different developmental schemes, phenotypes, antibody repertoires, localization and beh...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Wiley
1998
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/32947 |
| _version_ | 1855522407014465536 |
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| author | Chevallier, N. Berthelemy, M. Laine, V. Rhun, D. le Femenia, F. Polack, B. Naessens, Jan Levy, D. Schwartz-Cornil, I. |
| author_browse | Berthelemy, M. Chevallier, N. Femenia, F. Laine, V. Levy, D. Naessens, Jan Polack, B. Rhun, D. le Schwartz-Cornil, I. |
| author_facet | Chevallier, N. Berthelemy, M. Laine, V. Rhun, D. le Femenia, F. Polack, B. Naessens, Jan Levy, D. Schwartz-Cornil, I. |
| author_sort | Chevallier, N. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Two populations of B lymphocytes. B-1 (CD5- and or CD1 lb-) and B-2 (CD5- and CD1 lb-) cells have been described. In mice, which is the species of reference for B-1 and B-2 cell studies, these two subsets present different developmental schemes, phenotypes, antibody repertoires, localization and behaviours. Interestingly, in sheep. B cells rearrange their immunoglobulin (Ig) loci around the neonatal period, similarly to murine B-1 cells. However, the phenotype of the sheep B cells has not been characterized with regard to their developmental pathway. In this report, we show that two sheep B-cell subsets can be distinguished on the basis of CD1 lb expression. Relative to CD1 lb- B cells, the CD1 lb- B cells frequently co-express CD5. CD1 lc, higher levels of surface IgM (sIgM), show larger cell size and higher cell-cycling activity, and thus present a B-1 like phenotype. However, unlike murine B-1 cells, sheep B-1 like cells mainly localize in blood, display a higher propensity to spontaneous apoptosis relative to B-2-like cells, and proliferate after sIgM stimulation. Our data show that despite neonatal immunoglobulin loci rearrangements, sheep B cells do not all express a B-1 like phenotype. However. B-1 and B-2 like cells co-exist and present phenotypic and behavioural specificities. Nevertheless, sheep B-1 and B-2 like cells differ from the murine B-1 and B-2 cells in their cell behabiour. These subsets can thus not be considered as true homologues among species. |
| format | Journal Article |
| id | CGSpace32947 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1998 |
| publishDateRange | 1998 |
| publishDateSort | 1998 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace329472023-09-30T10:55:09Z B-1-like cells exist in sheep. Characterization of their phenotype and behaviour Chevallier, N. Berthelemy, M. Laine, V. Rhun, D. le Femenia, F. Polack, B. Naessens, Jan Levy, D. Schwartz-Cornil, I. sheep cells phenotypes leucocytes blood Two populations of B lymphocytes. B-1 (CD5- and or CD1 lb-) and B-2 (CD5- and CD1 lb-) cells have been described. In mice, which is the species of reference for B-1 and B-2 cell studies, these two subsets present different developmental schemes, phenotypes, antibody repertoires, localization and behaviours. Interestingly, in sheep. B cells rearrange their immunoglobulin (Ig) loci around the neonatal period, similarly to murine B-1 cells. However, the phenotype of the sheep B cells has not been characterized with regard to their developmental pathway. In this report, we show that two sheep B-cell subsets can be distinguished on the basis of CD1 lb expression. Relative to CD1 lb- B cells, the CD1 lb- B cells frequently co-express CD5. CD1 lc, higher levels of surface IgM (sIgM), show larger cell size and higher cell-cycling activity, and thus present a B-1 like phenotype. However, unlike murine B-1 cells, sheep B-1 like cells mainly localize in blood, display a higher propensity to spontaneous apoptosis relative to B-2-like cells, and proliferate after sIgM stimulation. Our data show that despite neonatal immunoglobulin loci rearrangements, sheep B cells do not all express a B-1 like phenotype. However. B-1 and B-2 like cells co-exist and present phenotypic and behavioural specificities. Nevertheless, sheep B-1 and B-2 like cells differ from the murine B-1 and B-2 cells in their cell behabiour. These subsets can thus not be considered as true homologues among species. 1998-10 2013-07-03T05:25:49Z 2013-07-03T05:25:49Z Journal Article https://hdl.handle.net/10568/32947 en Open Access Wiley Immunology;95(2): 178-184 |
| spellingShingle | sheep cells phenotypes leucocytes blood Chevallier, N. Berthelemy, M. Laine, V. Rhun, D. le Femenia, F. Polack, B. Naessens, Jan Levy, D. Schwartz-Cornil, I. B-1-like cells exist in sheep. Characterization of their phenotype and behaviour |
| title | B-1-like cells exist in sheep. Characterization of their phenotype and behaviour |
| title_full | B-1-like cells exist in sheep. Characterization of their phenotype and behaviour |
| title_fullStr | B-1-like cells exist in sheep. Characterization of their phenotype and behaviour |
| title_full_unstemmed | B-1-like cells exist in sheep. Characterization of their phenotype and behaviour |
| title_short | B-1-like cells exist in sheep. Characterization of their phenotype and behaviour |
| title_sort | b 1 like cells exist in sheep characterization of their phenotype and behaviour |
| topic | sheep cells phenotypes leucocytes blood |
| url | https://hdl.handle.net/10568/32947 |
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