Polymorphisms, in the Tnfa gene of different inbred mouse strains

In view of the Tnfa gene location within the major histocompatibility (MHC) region and reports of its importance in disease protection activity, this paper examines the TNF a genes of a number of different mouse strains which were known to be of different disease response phenotypes. Twelve inbred m...

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Detalles Bibliográficos
Autores principales: Iraqi, F.A., Teale, A.J.
Formato: Journal Article
Lenguaje:Inglés
Publicado: Springer 1999
Materias:
Acceso en línea:https://hdl.handle.net/10568/32943
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author Iraqi, F.A.
Teale, A.J.
author_browse Iraqi, F.A.
Teale, A.J.
author_facet Iraqi, F.A.
Teale, A.J.
author_sort Iraqi, F.A.
collection Repository of Agricultural Research Outputs (CGSpace)
description In view of the Tnfa gene location within the major histocompatibility (MHC) region and reports of its importance in disease protection activity, this paper examines the TNF a genes of a number of different mouse strains which were known to be of different disease response phenotypes. Twelve inbred mouse strains were studied. In a separate study, the repeat length in the (CA) n microsatellite located within the Tnfa promoter region of these 12 inbred mouse strains was determined, High relative molecular mass genomic (chramosomal) DNA of the inbred mouse strains was obtained from the Jackson Laboratory. The results of the study shows that pCR-RFLps were not detected in the promoter region. Two haplotypes were defined in the first intron and three haplotypes could be defined in 3 UTR in the 12 strains based on PCR-RFLP genotypes and, moreover, these were not strictly concordant with serologically defined H2 haplotypes.
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spelling CGSpace329432024-05-01T08:18:13Z Polymorphisms, in the Tnfa gene of different inbred mouse strains Iraqi, F.A. Teale, A.J. polymorphism dna genes mice In view of the Tnfa gene location within the major histocompatibility (MHC) region and reports of its importance in disease protection activity, this paper examines the TNF a genes of a number of different mouse strains which were known to be of different disease response phenotypes. Twelve inbred mouse strains were studied. In a separate study, the repeat length in the (CA) n microsatellite located within the Tnfa promoter region of these 12 inbred mouse strains was determined, High relative molecular mass genomic (chramosomal) DNA of the inbred mouse strains was obtained from the Jackson Laboratory. The results of the study shows that pCR-RFLps were not detected in the promoter region. Two haplotypes were defined in the first intron and three haplotypes could be defined in 3 UTR in the 12 strains based on PCR-RFLP genotypes and, moreover, these were not strictly concordant with serologically defined H2 haplotypes. 1999-01-21 2013-07-03T05:25:48Z 2013-07-03T05:25:48Z Journal Article https://hdl.handle.net/10568/32943 en Limited Access Springer Immunogenetics;49: 242-245
spellingShingle polymorphism
dna
genes
mice
Iraqi, F.A.
Teale, A.J.
Polymorphisms, in the Tnfa gene of different inbred mouse strains
title Polymorphisms, in the Tnfa gene of different inbred mouse strains
title_full Polymorphisms, in the Tnfa gene of different inbred mouse strains
title_fullStr Polymorphisms, in the Tnfa gene of different inbred mouse strains
title_full_unstemmed Polymorphisms, in the Tnfa gene of different inbred mouse strains
title_short Polymorphisms, in the Tnfa gene of different inbred mouse strains
title_sort polymorphisms in the tnfa gene of different inbred mouse strains
topic polymorphism
dna
genes
mice
url https://hdl.handle.net/10568/32943
work_keys_str_mv AT iraqifa polymorphismsinthetnfageneofdifferentinbredmousestrains
AT tealeaj polymorphismsinthetnfageneofdifferentinbredmousestrains