Characterisation of the QM gene of Trypanosoma brucei
The QM protein has been reported to have roles in both tumour suppression and transcription factor regulation in vertebrate cells, and in ribosome stability in both yeast and mammals. The present study isolated the QM gene of Trypanosoma brucei and determined its sequence. Alignment with QM sequence...
| Autores principales: | , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Oxford University Press
2002
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/32920 |
| _version_ | 1855536775076773888 |
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| author | Lillico, Simon G. Mottram, J.C. Murphy, N.B. Welburn, S.C. |
| author_browse | Lillico, Simon G. Mottram, J.C. Murphy, N.B. Welburn, S.C. |
| author_facet | Lillico, Simon G. Mottram, J.C. Murphy, N.B. Welburn, S.C. |
| author_sort | Lillico, Simon G. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The QM protein has been reported to have roles in both tumour suppression and transcription factor regulation in vertebrate cells, and in ribosome stability in both yeast and mammals. The present study isolated the QM gene of Trypanosoma brucei and determined its sequence. Alignment with QM sequences from Saccharomyces cerevisiae, Arabidopsis thaliana, Drosophila melanogaster and Homo sapiens revealed greater than 60% identity. Southern blot analysis revealed multiple copies of QM within the trypanosome genome. An epitope tag was inserted into the C-terminus of the T. brucei QM and the protein expressed under inducible control in procyclic form trypanosomes. Immune fluorescence microscopy revealed co-localisation with the GPI:protein transamidase component, GPI8, a distribution indicative of ribosome association with the rough endoplasmic reticulum. |
| format | Journal Article |
| id | CGSpace32920 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2002 |
| publishDateRange | 2002 |
| publishDateSort | 2002 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | CGSpace329202024-03-06T10:16:43Z Characterisation of the QM gene of Trypanosoma brucei Lillico, Simon G. Mottram, J.C. Murphy, N.B. Welburn, S.C. trypanosoma brucei genes proteins endoplasmic reticulum nucleotide sequence The QM protein has been reported to have roles in both tumour suppression and transcription factor regulation in vertebrate cells, and in ribosome stability in both yeast and mammals. The present study isolated the QM gene of Trypanosoma brucei and determined its sequence. Alignment with QM sequences from Saccharomyces cerevisiae, Arabidopsis thaliana, Drosophila melanogaster and Homo sapiens revealed greater than 60% identity. Southern blot analysis revealed multiple copies of QM within the trypanosome genome. An epitope tag was inserted into the C-terminus of the T. brucei QM and the protein expressed under inducible control in procyclic form trypanosomes. Immune fluorescence microscopy revealed co-localisation with the GPI:protein transamidase component, GPI8, a distribution indicative of ribosome association with the rough endoplasmic reticulum. 2002-06 2013-07-03T05:25:45Z 2013-07-03T05:25:45Z Journal Article https://hdl.handle.net/10568/32920 en Open Access Oxford University Press FEMS Microbiology Letters;211(2): 123-128 |
| spellingShingle | trypanosoma brucei genes proteins endoplasmic reticulum nucleotide sequence Lillico, Simon G. Mottram, J.C. Murphy, N.B. Welburn, S.C. Characterisation of the QM gene of Trypanosoma brucei |
| title | Characterisation of the QM gene of Trypanosoma brucei |
| title_full | Characterisation of the QM gene of Trypanosoma brucei |
| title_fullStr | Characterisation of the QM gene of Trypanosoma brucei |
| title_full_unstemmed | Characterisation of the QM gene of Trypanosoma brucei |
| title_short | Characterisation of the QM gene of Trypanosoma brucei |
| title_sort | characterisation of the qm gene of trypanosoma brucei |
| topic | trypanosoma brucei genes proteins endoplasmic reticulum nucleotide sequence |
| url | https://hdl.handle.net/10568/32920 |
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