Molecular characterization of live Theileria parva sporozoite vaccine stabilates reveals extensive genotypic diversity
The current Infection and Treatment Method of vaccination against East Coast fever comprises an inoculation of live Theileria parva sporozoites and simultaneous administration of oxytetracycline. Immunization with a combination of parasite types has been shown to provide broader protection than inoc...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2011
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/3166 |
| _version_ | 1855521650563350528 |
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| author | Patel, Ekta Lubembe, D.M. Gachanja, J. Mwaura, S. Spooner, P.R. Toye, Philip G. |
| author_browse | Gachanja, J. Lubembe, D.M. Mwaura, S. Patel, Ekta Spooner, P.R. Toye, Philip G. |
| author_facet | Patel, Ekta Lubembe, D.M. Gachanja, J. Mwaura, S. Spooner, P.R. Toye, Philip G. |
| author_sort | Patel, Ekta |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The current Infection and Treatment Method of vaccination against East Coast fever comprises an inoculation of live Theileria parva sporozoites and simultaneous administration of oxytetracycline. Immunization with a combination of parasite types has been shown to provide broader protection than inoculation of individual strains. In this study, we used a high-throughput capillary electrophoresis system to determine the genotypic composition of the Muguga Cocktail, a widely used vaccine stabilate derived from three seed stabilates - Muguga, Serengeti-transformed and Kiambu 5. Five satellite markers were used to genotype the vaccine and reference stabilates from two commercial-scale preparations of the vaccine. In addition, 224 cloned cell lines established by infection of bovine lymphocytes with T. parva parasites from the component stabilates were genotyped. The results indicate that, for the recently prepared batch, there are at least eight genotypes in each of the Muguga and the Serengeti-transformed stabilates, whilst parasites from the Kiambu 5 stabilate showed no diversity at the five loci. The Serengeti-transformed stabilate contained parasites of the Kiambu 5 genotype and of two genotypes present in the Muguga stabilate, whereas there were no genotypes common to the Muguga and Kiambu 5 stabilates. When stabilates from the two vaccine batches were compared, no allelic variations were identified between the Muguga and Kiambu 5 parasites, whilst lack of sufficient clones prevented a full comparison of the Serengeti-transformed stabilates. The findings will facilitate examination of the extent to which the vaccine strains become resident in areas under vaccination, the identification of ‘breakthrough’ strains and the establishment of the quality assurance protocols to detect variations in the production of the vaccine. The cloned cell lines will be useful for further understanding the antigenic diversity of parasites in the vaccine. |
| format | Journal Article |
| id | CGSpace3166 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2011 |
| publishDateRange | 2011 |
| publishDateSort | 2011 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace31662025-06-13T04:20:12Z Molecular characterization of live Theileria parva sporozoite vaccine stabilates reveals extensive genotypic diversity Patel, Ekta Lubembe, D.M. Gachanja, J. Mwaura, S. Spooner, P.R. Toye, Philip G. theileria parva east coast fever vaccines disease control The current Infection and Treatment Method of vaccination against East Coast fever comprises an inoculation of live Theileria parva sporozoites and simultaneous administration of oxytetracycline. Immunization with a combination of parasite types has been shown to provide broader protection than inoculation of individual strains. In this study, we used a high-throughput capillary electrophoresis system to determine the genotypic composition of the Muguga Cocktail, a widely used vaccine stabilate derived from three seed stabilates - Muguga, Serengeti-transformed and Kiambu 5. Five satellite markers were used to genotype the vaccine and reference stabilates from two commercial-scale preparations of the vaccine. In addition, 224 cloned cell lines established by infection of bovine lymphocytes with T. parva parasites from the component stabilates were genotyped. The results indicate that, for the recently prepared batch, there are at least eight genotypes in each of the Muguga and the Serengeti-transformed stabilates, whilst parasites from the Kiambu 5 stabilate showed no diversity at the five loci. The Serengeti-transformed stabilate contained parasites of the Kiambu 5 genotype and of two genotypes present in the Muguga stabilate, whereas there were no genotypes common to the Muguga and Kiambu 5 stabilates. When stabilates from the two vaccine batches were compared, no allelic variations were identified between the Muguga and Kiambu 5 parasites, whilst lack of sufficient clones prevented a full comparison of the Serengeti-transformed stabilates. The findings will facilitate examination of the extent to which the vaccine strains become resident in areas under vaccination, the identification of ‘breakthrough’ strains and the establishment of the quality assurance protocols to detect variations in the production of the vaccine. The cloned cell lines will be useful for further understanding the antigenic diversity of parasites in the vaccine. 2011-06 2011-02-10T09:19:28Z 2011-02-10T09:19:28Z Journal Article https://hdl.handle.net/10568/3166 en Limited Access Elsevier Patel, E.H., Lubembe, D.M., Gachanja, J., Mwaura, S., Spooner, P. and Toye, P. 2011. Molecular characterization of live Theileria parva sporozoite vaccine stabilates reveals extensive genotypic diversity. Veterinary Parasitology 179(1-3):62-68. |
| spellingShingle | theileria parva east coast fever vaccines disease control Patel, Ekta Lubembe, D.M. Gachanja, J. Mwaura, S. Spooner, P.R. Toye, Philip G. Molecular characterization of live Theileria parva sporozoite vaccine stabilates reveals extensive genotypic diversity |
| title | Molecular characterization of live Theileria parva sporozoite vaccine stabilates reveals extensive genotypic diversity |
| title_full | Molecular characterization of live Theileria parva sporozoite vaccine stabilates reveals extensive genotypic diversity |
| title_fullStr | Molecular characterization of live Theileria parva sporozoite vaccine stabilates reveals extensive genotypic diversity |
| title_full_unstemmed | Molecular characterization of live Theileria parva sporozoite vaccine stabilates reveals extensive genotypic diversity |
| title_short | Molecular characterization of live Theileria parva sporozoite vaccine stabilates reveals extensive genotypic diversity |
| title_sort | molecular characterization of live theileria parva sporozoite vaccine stabilates reveals extensive genotypic diversity |
| topic | theileria parva east coast fever vaccines disease control |
| url | https://hdl.handle.net/10568/3166 |
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