Inductive requirements of Theileria parva-specific cytotoxic T lymphocytes
This paper determines whether contact between parasite-specific CD4+ T cells and CD8+ T cells is an essential requirement for helper function, additional experiments were performed using two-chamber culture plates incorporating semi-porous membranes. TpM were co-cultured with naive or immune CD8 + T...
| Main Authors: | , |
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| Format: | Conference Paper |
| Language: | Inglés |
| Published: |
International Laboratory for Research on Animal Diseases
1995
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/3131 |
| _version_ | 1855524100949147648 |
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| author | Taracha, E.L.N. McKeever, Declan J. |
| author_browse | McKeever, Declan J. Taracha, E.L.N. |
| author_facet | Taracha, E.L.N. McKeever, Declan J. |
| author_sort | Taracha, E.L.N. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | This paper determines whether contact between parasite-specific CD4+ T cells and CD8+ T cells is an essential requirement for helper function, additional experiments were performed using two-chamber culture plates incorporating semi-porous membranes. TpM were co-cultured with naive or immune CD8 + T cells in the outer chamber and with immune DC4 + T cells in the inner chamber. Cytotoxic activity was observed in cultures containing primed CD8 + T cells but was undetectable in those containing naive CD8 + T cells. This abservations suggests that while soluble factors derived from activated CD4 + T helper cells are sufficient for the activation of memory CTL, naive CD8 + T cells require actual cell contact. Whether this signifies the involvement of receptor-ligand interactions or is a reflection of a requirement for defined local cytokine concentrations is not clear. |
| format | Conference Paper |
| id | CGSpace3131 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1995 |
| publishDateRange | 1995 |
| publishDateSort | 1995 |
| publisher | International Laboratory for Research on Animal Diseases |
| publisherStr | International Laboratory for Research on Animal Diseases |
| record_format | dspace |
| spelling | CGSpace31312023-12-21T14:51:32Z Inductive requirements of Theileria parva-specific cytotoxic T lymphocytes Taracha, E.L.N. McKeever, Declan J. theileria parva animal diseases metastigmata lymphocytes cattle This paper determines whether contact between parasite-specific CD4+ T cells and CD8+ T cells is an essential requirement for helper function, additional experiments were performed using two-chamber culture plates incorporating semi-porous membranes. TpM were co-cultured with naive or immune CD8 + T cells in the outer chamber and with immune DC4 + T cells in the inner chamber. Cytotoxic activity was observed in cultures containing primed CD8 + T cells but was undetectable in those containing naive CD8 + T cells. This abservations suggests that while soluble factors derived from activated CD4 + T helper cells are sufficient for the activation of memory CTL, naive CD8 + T cells require actual cell contact. Whether this signifies the involvement of receptor-ligand interactions or is a reflection of a requirement for defined local cytokine concentrations is not clear. 1995 2011-02-04T12:28:50Z 2011-02-04T12:28:50Z Conference Paper https://hdl.handle.net/10568/3131 en Open Access International Laboratory for Research on Animal Diseases |
| spellingShingle | theileria parva animal diseases metastigmata lymphocytes cattle Taracha, E.L.N. McKeever, Declan J. Inductive requirements of Theileria parva-specific cytotoxic T lymphocytes |
| title | Inductive requirements of Theileria parva-specific cytotoxic T lymphocytes |
| title_full | Inductive requirements of Theileria parva-specific cytotoxic T lymphocytes |
| title_fullStr | Inductive requirements of Theileria parva-specific cytotoxic T lymphocytes |
| title_full_unstemmed | Inductive requirements of Theileria parva-specific cytotoxic T lymphocytes |
| title_short | Inductive requirements of Theileria parva-specific cytotoxic T lymphocytes |
| title_sort | inductive requirements of theileria parva specific cytotoxic t lymphocytes |
| topic | theileria parva animal diseases metastigmata lymphocytes cattle |
| url | https://hdl.handle.net/10568/3131 |
| work_keys_str_mv | AT tarachaeln inductiverequirementsoftheileriaparvaspecificcytotoxictlymphocytes AT mckeeverdeclanj inductiverequirementsoftheileriaparvaspecificcytotoxictlymphocytes |