Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes
We have developed two loop-mediated isothermal amplification (LAMP) assays for the detection of Theileria parva, the causative agent of East Coast fever (ECF), an economically important cattle disease in eastern, central and southern Africa. These assays target the polymorphic immunodominant molecul...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Elsevier
2010
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/308 |
| _version_ | 1855524969458434048 |
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| author | Thekisoe, O.M.M. Rambritch, N.E. Nakao, R. Bazie, R.S. Mbati, P. Namangala, B. Malele, I. Skilton, Robert A. Jongejan, F. Sugimoto, C. Kawazu, S.I. Inoue, N. |
| author_browse | Bazie, R.S. Inoue, N. Jongejan, F. Kawazu, S.I. Malele, I. Mbati, P. Nakao, R. Namangala, B. Rambritch, N.E. Skilton, Robert A. Sugimoto, C. Thekisoe, O.M.M. |
| author_facet | Thekisoe, O.M.M. Rambritch, N.E. Nakao, R. Bazie, R.S. Mbati, P. Namangala, B. Malele, I. Skilton, Robert A. Jongejan, F. Sugimoto, C. Kawazu, S.I. Inoue, N. |
| author_sort | Thekisoe, O.M.M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | We have developed two loop-mediated isothermal amplification (LAMP) assays for the detection of Theileria parva, the causative agent of East Coast fever (ECF), an economically important cattle disease in eastern, central and southern Africa. These assays target the polymorphic immunodominant molecule (PIM) and p150 LAMP genes. The primer set for each gene target consists of six primers, and each set recognises eight distinct regions on the target gene to give highly specific detection of T. parva. The detection limit of each primer set is 1 fg, which is equivalent to one copy of the PIM and p150 T. parva genes. These PIM and p150 LAMP primer sets amplify DNA of T. parva isolates from cattle and buffalo from different countries including Kenya, South Africa, Tanzania, Rwanda, Uganda and Burundi, indicating their ability to detect T. parva from different countries. With the advantages of simplicity, rapidity and cost effectiveness, these LAMP assays are good candidates for molecular epidemiology studies and for monitoring control programs in ECF-endemic, resource poor countries. |
| format | Journal Article |
| id | CGSpace308 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2010 |
| publishDateRange | 2010 |
| publishDateSort | 2010 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace3082024-01-09T09:04:44Z Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes Thekisoe, O.M.M. Rambritch, N.E. Nakao, R. Bazie, R.S. Mbati, P. Namangala, B. Malele, I. Skilton, Robert A. Jongejan, F. Sugimoto, C. Kawazu, S.I. Inoue, N. theileria parva east coast fever We have developed two loop-mediated isothermal amplification (LAMP) assays for the detection of Theileria parva, the causative agent of East Coast fever (ECF), an economically important cattle disease in eastern, central and southern Africa. These assays target the polymorphic immunodominant molecule (PIM) and p150 LAMP genes. The primer set for each gene target consists of six primers, and each set recognises eight distinct regions on the target gene to give highly specific detection of T. parva. The detection limit of each primer set is 1 fg, which is equivalent to one copy of the PIM and p150 T. parva genes. These PIM and p150 LAMP primer sets amplify DNA of T. parva isolates from cattle and buffalo from different countries including Kenya, South Africa, Tanzania, Rwanda, Uganda and Burundi, indicating their ability to detect T. parva from different countries. With the advantages of simplicity, rapidity and cost effectiveness, these LAMP assays are good candidates for molecular epidemiology studies and for monitoring control programs in ECF-endemic, resource poor countries. 2010-01 2009-12-30T09:00:14Z 2009-12-30T09:00:14Z Journal Article https://hdl.handle.net/10568/308 en Limited Access Elsevier Thekisoe, O.M.M.; Rambritch, N.E.; Nakao, R.; Bazie, R.S.; Mbati, P.; Namangala, B.; Malele, I.; Skilton, R.A.; Jongejan, F.; Sugimoto, C.; Kawazu, S.I.; Inoue, N. 2009. Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes. International Journal for Parasitology. v. 40(1). p. 55-61. |
| spellingShingle | theileria parva east coast fever Thekisoe, O.M.M. Rambritch, N.E. Nakao, R. Bazie, R.S. Mbati, P. Namangala, B. Malele, I. Skilton, Robert A. Jongejan, F. Sugimoto, C. Kawazu, S.I. Inoue, N. Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes |
| title | Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes |
| title_full | Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes |
| title_fullStr | Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes |
| title_full_unstemmed | Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes |
| title_short | Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes |
| title_sort | loop mediated isothermal amplification lamp assays for detection of theileria parva infections targeting the pim and p150 genes |
| topic | theileria parva east coast fever |
| url | https://hdl.handle.net/10568/308 |
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