Use of an in vivo system to determine the G418 resistance phenotype of bloodstream-form Trypanosoma brucei brucei transfectants
We determined the level of susceptibility of Trypanosoma brucei brucei to the aminoglycoside G418 in vivo and demonstrated that it is possible to select for G418-resistant transfected T. brucei brucei bloodstream parasites in a mouse host by inoculating the drug intraperitoneally at doses between 40...
| Main Authors: | , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
American Society for Microbiology
1993
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/30080 |
| _version_ | 1855519048823996416 |
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| author | Murphy, N.B. Muthiani, M. Peregrine, A.S. |
| author_browse | Murphy, N.B. Muthiani, M. Peregrine, A.S. |
| author_facet | Murphy, N.B. Muthiani, M. Peregrine, A.S. |
| author_sort | Murphy, N.B. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | We determined the level of susceptibility of Trypanosoma brucei brucei to the aminoglycoside G418 in vivo and demonstrated that it is possible to select for G418-resistant transfected T. brucei brucei bloodstream parasites in a mouse host by inoculating the drug intraperitoneally at doses between 40 and 80 mg/kg of body weight daily for 3 days. The ability to select for transfectants in vivo offers new possibilities for studies on genetic recombination in these parasites. |
| format | Journal Article |
| id | CGSpace30080 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1993 |
| publishDateRange | 1993 |
| publishDateSort | 1993 |
| publisher | American Society for Microbiology |
| publisherStr | American Society for Microbiology |
| record_format | dspace |
| spelling | CGSpace300802024-05-01T08:15:54Z Use of an in vivo system to determine the G418 resistance phenotype of bloodstream-form Trypanosoma brucei brucei transfectants Murphy, N.B. Muthiani, M. Peregrine, A.S. trypanosoma brucei infectious diseases pharmacology We determined the level of susceptibility of Trypanosoma brucei brucei to the aminoglycoside G418 in vivo and demonstrated that it is possible to select for G418-resistant transfected T. brucei brucei bloodstream parasites in a mouse host by inoculating the drug intraperitoneally at doses between 40 and 80 mg/kg of body weight daily for 3 days. The ability to select for transfectants in vivo offers new possibilities for studies on genetic recombination in these parasites. 1993-05 2013-06-11T09:26:08Z 2013-06-11T09:26:08Z Journal Article https://hdl.handle.net/10568/30080 en Open Access American Society for Microbiology Antimicrobial Agents and Chemotherapy;37: 1167-1170 |
| spellingShingle | trypanosoma brucei infectious diseases pharmacology Murphy, N.B. Muthiani, M. Peregrine, A.S. Use of an in vivo system to determine the G418 resistance phenotype of bloodstream-form Trypanosoma brucei brucei transfectants |
| title | Use of an in vivo system to determine the G418 resistance phenotype of bloodstream-form Trypanosoma brucei brucei transfectants |
| title_full | Use of an in vivo system to determine the G418 resistance phenotype of bloodstream-form Trypanosoma brucei brucei transfectants |
| title_fullStr | Use of an in vivo system to determine the G418 resistance phenotype of bloodstream-form Trypanosoma brucei brucei transfectants |
| title_full_unstemmed | Use of an in vivo system to determine the G418 resistance phenotype of bloodstream-form Trypanosoma brucei brucei transfectants |
| title_short | Use of an in vivo system to determine the G418 resistance phenotype of bloodstream-form Trypanosoma brucei brucei transfectants |
| title_sort | use of an in vivo system to determine the g418 resistance phenotype of bloodstream form trypanosoma brucei brucei transfectants |
| topic | trypanosoma brucei infectious diseases pharmacology |
| url | https://hdl.handle.net/10568/30080 |
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