Satellite DNa polymorphisms and AFLP correlate with Bos indicus-taurus hybridization

We describe satellite DNA variation that detects hybridization of Bos indicus (Zebu or indicine cattle) and Bos taurus (taurine cattle) in African cattle populations. On southern blots hybridized to a satellite III probe, relative intensities of Hinf1 fragments correlated with the taurine-zebu compo...

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Autores principales: Nijman, I.J., Bradley, D.G., Hanotte, Olivier H., Otsen, M., Lenstra, J.A.
Formato: Journal Article
Lenguaje:Inglés
Publicado: Wiley 1999
Materias:
Acceso en línea:https://hdl.handle.net/10568/30066
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author Nijman, I.J.
Bradley, D.G.
Hanotte, Olivier H.
Otsen, M.
Lenstra, J.A.
author_browse Bradley, D.G.
Hanotte, Olivier H.
Lenstra, J.A.
Nijman, I.J.
Otsen, M.
author_facet Nijman, I.J.
Bradley, D.G.
Hanotte, Olivier H.
Otsen, M.
Lenstra, J.A.
author_sort Nijman, I.J.
collection Repository of Agricultural Research Outputs (CGSpace)
description We describe satellite DNA variation that detects hybridization of Bos indicus (Zebu or indicine cattle) and Bos taurus (taurine cattle) in African cattle populations. On southern blots hybridized to a satellite III probe, relative intensities of Hinf1 fragments correlated with the taurine-zebu composition in hybrid animals as deduced from AFLP genotyping of the same animals and previous data on microsatellite allele frequencies. Similar results were obtained by PCR-RFLP analysis of a zebu-specific mutation in the repeat unit of satellite 1.711b. analysis of individulas from 20 African cattle breeds indicate that the centromeric satellites of the sanga breeds are of the taurine type and that several East-African zebu breeds are hybrids between taurine and zebu. These satellite RFLP or SFLP, markers provide a fast method to screen the genetic makeup of African cattle.
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spelling CGSpace300662023-09-09T14:17:03Z Satellite DNa polymorphisms and AFLP correlate with Bos indicus-taurus hybridization Nijman, I.J. Bradley, D.G. Hanotte, Olivier H. Otsen, M. Lenstra, J.A. cattle dna polymorphism hybridization bos indicus cattle bos taurus cattle breeds dna fingerprinting nucleotides We describe satellite DNA variation that detects hybridization of Bos indicus (Zebu or indicine cattle) and Bos taurus (taurine cattle) in African cattle populations. On southern blots hybridized to a satellite III probe, relative intensities of Hinf1 fragments correlated with the taurine-zebu composition in hybrid animals as deduced from AFLP genotyping of the same animals and previous data on microsatellite allele frequencies. Similar results were obtained by PCR-RFLP analysis of a zebu-specific mutation in the repeat unit of satellite 1.711b. analysis of individulas from 20 African cattle breeds indicate that the centromeric satellites of the sanga breeds are of the taurine type and that several East-African zebu breeds are hybrids between taurine and zebu. These satellite RFLP or SFLP, markers provide a fast method to screen the genetic makeup of African cattle. 1999-08 2013-06-11T09:26:04Z 2013-06-11T09:26:04Z Journal Article https://hdl.handle.net/10568/30066 en Limited Access Wiley Animal Genetics;30(4): 265-273
spellingShingle cattle
dna
polymorphism
hybridization
bos indicus cattle
bos taurus cattle
breeds
dna fingerprinting
nucleotides
Nijman, I.J.
Bradley, D.G.
Hanotte, Olivier H.
Otsen, M.
Lenstra, J.A.
Satellite DNa polymorphisms and AFLP correlate with Bos indicus-taurus hybridization
title Satellite DNa polymorphisms and AFLP correlate with Bos indicus-taurus hybridization
title_full Satellite DNa polymorphisms and AFLP correlate with Bos indicus-taurus hybridization
title_fullStr Satellite DNa polymorphisms and AFLP correlate with Bos indicus-taurus hybridization
title_full_unstemmed Satellite DNa polymorphisms and AFLP correlate with Bos indicus-taurus hybridization
title_short Satellite DNa polymorphisms and AFLP correlate with Bos indicus-taurus hybridization
title_sort satellite dna polymorphisms and aflp correlate with bos indicus taurus hybridization
topic cattle
dna
polymorphism
hybridization
bos indicus cattle
bos taurus cattle
breeds
dna fingerprinting
nucleotides
url https://hdl.handle.net/10568/30066
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