Linkage mapping of the bovine lysosomal alphamannosidase (MANB) gene
Lysosomal alpha-mannosidase is involved in the metabolism of mannoses in oligosaccharides in lysosomes. Deficiency of alpha-mannosidase, alpha-mannosidosis, has been described as an autosomal recessive storage disease in angus cattle, Galloway cattle and in cats. It is caused by non-synonymous subst...
| Autores principales: | , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
1999
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/30064 |
| Sumario: | Lysosomal alpha-mannosidase is involved in the metabolism of mannoses in oligosaccharides in lysosomes. Deficiency of alpha-mannosidase, alpha-mannosidosis, has been described as an autosomal recessive storage disease in angus cattle, Galloway cattle and in cats. It is caused by non-synonymous substitutions in the encoding gene (MANB). Primers for MANB were designed from a bovine sequence (Gen Bank accession number U97686) and amplified a 316-bp fragment comprising intron 2 and adjacent exonic sequences. This paper discusses PCR primers, PCR conditions and polymorphism detection; and allele frequencies, inheritance and chromosomal location. |
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