A PCR - based test for the presence of endogenous virus gene evA in chickens
An endogenous virus, denoted evA, is present at high frequency in all brown egg layer lines. Using inverse polymerase chain reaction (PCR) based on the viral LTR regions, products were obtained containing cellular sequences 5' and 3' to the viral insertion point. PCR of chicken genomic DNA was carri...
| Autores principales: | , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
1997
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/30062 |
| _version_ | 1855514744474042368 |
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| author | Nave, A. Iraqi, F.A. Khatib, H. Soller, M. |
| author_browse | Iraqi, F.A. Khatib, H. Nave, A. Soller, M. |
| author_facet | Nave, A. Iraqi, F.A. Khatib, H. Soller, M. |
| author_sort | Nave, A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | An endogenous virus, denoted evA, is present at high frequency in all brown egg layer lines. Using inverse polymerase chain reaction (PCR) based on the viral LTR regions, products were obtained containing cellular sequences 5' and 3' to the viral insertion point. PCR of chicken genomic DNA was carried out, using primers chosen from the 5' and 3' cellular sequences and a primer chosen from either the U3 or U5 portions of the viral LTR. Amplification of DNA from birds that did not carry evA with the primer triplets always gave a single 364 bp reaction product, interpreted as representing the flank-to-flank amplification product. Amplification of DNA from known homozygous or heterozygous evA carriers, with the same primer triplets, always gave both the expected junction product and 364 bp product. Therefore, these primer sequences can be used to distinguish evA carriers from non-carriers but cannot distinguish between homozygous and heterozygous evA carriers. |
| format | Journal Article |
| id | CGSpace30062 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1997 |
| publishDateRange | 1997 |
| publishDateSort | 1997 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace300622024-05-01T08:15:18Z A PCR - based test for the presence of endogenous virus gene evA in chickens Nave, A. Iraqi, F.A. Khatib, H. Soller, M. chickens viruses pcr genetics An endogenous virus, denoted evA, is present at high frequency in all brown egg layer lines. Using inverse polymerase chain reaction (PCR) based on the viral LTR regions, products were obtained containing cellular sequences 5' and 3' to the viral insertion point. PCR of chicken genomic DNA was carried out, using primers chosen from the 5' and 3' cellular sequences and a primer chosen from either the U3 or U5 portions of the viral LTR. Amplification of DNA from birds that did not carry evA with the primer triplets always gave a single 364 bp reaction product, interpreted as representing the flank-to-flank amplification product. Amplification of DNA from known homozygous or heterozygous evA carriers, with the same primer triplets, always gave both the expected junction product and 364 bp product. Therefore, these primer sequences can be used to distinguish evA carriers from non-carriers but cannot distinguish between homozygous and heterozygous evA carriers. 1997-02 2013-06-11T09:26:04Z 2013-06-11T09:26:04Z Journal Article https://hdl.handle.net/10568/30062 en Limited Access Wiley Animal Genetics;28: 46-48 |
| spellingShingle | chickens viruses pcr genetics Nave, A. Iraqi, F.A. Khatib, H. Soller, M. A PCR - based test for the presence of endogenous virus gene evA in chickens |
| title | A PCR - based test for the presence of endogenous virus gene evA in chickens |
| title_full | A PCR - based test for the presence of endogenous virus gene evA in chickens |
| title_fullStr | A PCR - based test for the presence of endogenous virus gene evA in chickens |
| title_full_unstemmed | A PCR - based test for the presence of endogenous virus gene evA in chickens |
| title_short | A PCR - based test for the presence of endogenous virus gene evA in chickens |
| title_sort | pcr based test for the presence of endogenous virus gene eva in chickens |
| topic | chickens viruses pcr genetics |
| url | https://hdl.handle.net/10568/30062 |
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