The effects of drug-sensitive and drug-resistant Trypanosoma congolense infections on the pharmacokinetics of homidium in Boran cattle
Two groups of five Boran (Bos indicus) cattle were infected with one of two populations of Trypanosoma congolense; one drug-sensitive (IL1180), and one drug-resistant (IL3330). The animals were then treated intramuscularly with homidium bromide at a dose rate of 1.0 mg kg - bodyweight 7 days after t...
| Autores principales: | , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2002
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/29979 |
| _version_ | 1855526515272318976 |
|---|---|
| author | Murilla, G.A. Peregrine, A.S. Ndung'u, J.M. Holmes, P.H. Eisler, M.C. |
| author_browse | Eisler, M.C. Holmes, P.H. Murilla, G.A. Ndung'u, J.M. Peregrine, A.S. |
| author_facet | Murilla, G.A. Peregrine, A.S. Ndung'u, J.M. Holmes, P.H. Eisler, M.C. |
| author_sort | Murilla, G.A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Two groups of five Boran (Bos indicus) cattle were infected with one of two populations of Trypanosoma congolense; one drug-sensitive (IL1180), and one drug-resistant (IL3330). The animals were then treated intramuscularly with homidium bromide at a dose rate of 1.0 mg kg - bodyweight 7 days after trypanosomes were detected in the peripheral blood of all the five animals in each grouFollowing treatment of cattle infected with drug-sensitive trypanosomes. parasites could no longer be detected in the bloodstream of four out of five cattle after 24 h, and after 48 h for the fifth animal. The animals remained aparasitaemic up to the end of the observation period of 90 days and serum drug concentrations determined by enzyme-linked immunosorbent assay (ELISA) remained above the detection limit of 0.1 ng ml for the entire period. Following treatment of cattle infected with drug-resistant trypanosomes. parasites did not disappear from the bloodstream in any of the five animals. The rate of drug elimination was greater in cattle infected with drug-resistant trypanosomes and the drug was no longer detectable approximately 3 weeks after treatment. Non-compartmental pharmacokinetic analysis showed that the values for t1 beta, of 75.5 ± 16.9 h, the area under the curve (AUC a-x ) of 1.33 ± 0.156 mce g h ml - and the MRT a-x of 32.8 ± 4.45 h obtained in cattle infected with the drug-resistant trypanosome population were significantly lower than the values of 424 ± 146 h for tJl, 1.67 ± 0.233 mug h ml -1 for AUC a-x and 297 ± 159 h for MRT a-x obtained in cattle infected with the drug sensitive population. The persistence of drug-resistant infections in cattle following homidium treatment was associated with more rapid drug elimination than in those in which infections with drug-sensitive parasites were cleared by the drug. |
| format | Journal Article |
| id | CGSpace29979 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2002 |
| publishDateRange | 2002 |
| publishDateSort | 2002 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace299792024-04-25T06:01:00Z The effects of drug-sensitive and drug-resistant Trypanosoma congolense infections on the pharmacokinetics of homidium in Boran cattle Murilla, G.A. Peregrine, A.S. Ndung'u, J.M. Holmes, P.H. Eisler, M.C. trypanosoma congolense drug resistance cattle elisa medicinal properties drugs Two groups of five Boran (Bos indicus) cattle were infected with one of two populations of Trypanosoma congolense; one drug-sensitive (IL1180), and one drug-resistant (IL3330). The animals were then treated intramuscularly with homidium bromide at a dose rate of 1.0 mg kg - bodyweight 7 days after trypanosomes were detected in the peripheral blood of all the five animals in each grouFollowing treatment of cattle infected with drug-sensitive trypanosomes. parasites could no longer be detected in the bloodstream of four out of five cattle after 24 h, and after 48 h for the fifth animal. The animals remained aparasitaemic up to the end of the observation period of 90 days and serum drug concentrations determined by enzyme-linked immunosorbent assay (ELISA) remained above the detection limit of 0.1 ng ml for the entire period. Following treatment of cattle infected with drug-resistant trypanosomes. parasites did not disappear from the bloodstream in any of the five animals. The rate of drug elimination was greater in cattle infected with drug-resistant trypanosomes and the drug was no longer detectable approximately 3 weeks after treatment. Non-compartmental pharmacokinetic analysis showed that the values for t1 beta, of 75.5 ± 16.9 h, the area under the curve (AUC a-x ) of 1.33 ± 0.156 mce g h ml - and the MRT a-x of 32.8 ± 4.45 h obtained in cattle infected with the drug-resistant trypanosome population were significantly lower than the values of 424 ± 146 h for tJl, 1.67 ± 0.233 mug h ml -1 for AUC a-x and 297 ± 159 h for MRT a-x obtained in cattle infected with the drug sensitive population. The persistence of drug-resistant infections in cattle following homidium treatment was associated with more rapid drug elimination than in those in which infections with drug-sensitive parasites were cleared by the drug. 2002-03 2013-06-11T09:25:41Z 2013-06-11T09:25:41Z Journal Article https://hdl.handle.net/10568/29979 en Limited Access Elsevier Acta Tropica;81(3): 185-195 |
| spellingShingle | trypanosoma congolense drug resistance cattle elisa medicinal properties drugs Murilla, G.A. Peregrine, A.S. Ndung'u, J.M. Holmes, P.H. Eisler, M.C. The effects of drug-sensitive and drug-resistant Trypanosoma congolense infections on the pharmacokinetics of homidium in Boran cattle |
| title | The effects of drug-sensitive and drug-resistant Trypanosoma congolense infections on the pharmacokinetics of homidium in Boran cattle |
| title_full | The effects of drug-sensitive and drug-resistant Trypanosoma congolense infections on the pharmacokinetics of homidium in Boran cattle |
| title_fullStr | The effects of drug-sensitive and drug-resistant Trypanosoma congolense infections on the pharmacokinetics of homidium in Boran cattle |
| title_full_unstemmed | The effects of drug-sensitive and drug-resistant Trypanosoma congolense infections on the pharmacokinetics of homidium in Boran cattle |
| title_short | The effects of drug-sensitive and drug-resistant Trypanosoma congolense infections on the pharmacokinetics of homidium in Boran cattle |
| title_sort | effects of drug sensitive and drug resistant trypanosoma congolense infections on the pharmacokinetics of homidium in boran cattle |
| topic | trypanosoma congolense drug resistance cattle elisa medicinal properties drugs |
| url | https://hdl.handle.net/10568/29979 |
| work_keys_str_mv | AT murillaga theeffectsofdrugsensitiveanddrugresistanttrypanosomacongolenseinfectionsonthepharmacokineticsofhomidiuminborancattle AT peregrineas theeffectsofdrugsensitiveanddrugresistanttrypanosomacongolenseinfectionsonthepharmacokineticsofhomidiuminborancattle AT ndungujm theeffectsofdrugsensitiveanddrugresistanttrypanosomacongolenseinfectionsonthepharmacokineticsofhomidiuminborancattle AT holmesph theeffectsofdrugsensitiveanddrugresistanttrypanosomacongolenseinfectionsonthepharmacokineticsofhomidiuminborancattle AT eislermc theeffectsofdrugsensitiveanddrugresistanttrypanosomacongolenseinfectionsonthepharmacokineticsofhomidiuminborancattle AT murillaga effectsofdrugsensitiveanddrugresistanttrypanosomacongolenseinfectionsonthepharmacokineticsofhomidiuminborancattle AT peregrineas effectsofdrugsensitiveanddrugresistanttrypanosomacongolenseinfectionsonthepharmacokineticsofhomidiuminborancattle AT ndungujm effectsofdrugsensitiveanddrugresistanttrypanosomacongolenseinfectionsonthepharmacokineticsofhomidiuminborancattle AT holmesph effectsofdrugsensitiveanddrugresistanttrypanosomacongolenseinfectionsonthepharmacokineticsofhomidiuminborancattle AT eislermc effectsofdrugsensitiveanddrugresistanttrypanosomacongolenseinfectionsonthepharmacokineticsofhomidiuminborancattle |