Cowdria ruminantium infection in ticks in the Kruger National Park

Adult Amblyomma hebraeum ticks, the principle vector of heartwater (cowdriosis) of domestic ruminants in southern Africa, were collected in pheromone traps placed in Kruger National Park, an exclusively wildlife sanctuary in South Africa. These ticks transmitted Cowdria ruminantium, the rickettsial...

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Autores principales: Peter, T.F., Bryson, N.R., Perry, Brian D., O'Callaghan, C.J., Medley, G.F., Smith, G.E., Mlambo, G., Horak, I.G., Burridge, M.J., Mahan, S.M.
Formato: Journal Article
Lenguaje:Inglés
Publicado: Wiley 1999
Materias:
Acceso en línea:https://hdl.handle.net/10568/29920
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author Peter, T.F.
Bryson, N.R.
Perry, Brian D.
O'Callaghan, C.J.
Medley, G.F.
Smith, G.E.
Mlambo, G.
Horak, I.G.
Burridge, M.J.
Mahan, S.M.
author_browse Bryson, N.R.
Burridge, M.J.
Horak, I.G.
Mahan, S.M.
Medley, G.F.
Mlambo, G.
O'Callaghan, C.J.
Perry, Brian D.
Peter, T.F.
Smith, G.E.
author_facet Peter, T.F.
Bryson, N.R.
Perry, Brian D.
O'Callaghan, C.J.
Medley, G.F.
Smith, G.E.
Mlambo, G.
Horak, I.G.
Burridge, M.J.
Mahan, S.M.
author_sort Peter, T.F.
collection Repository of Agricultural Research Outputs (CGSpace)
description Adult Amblyomma hebraeum ticks, the principle vector of heartwater (cowdriosis) of domestic ruminants in southern Africa, were collected in pheromone traps placed in Kruger National Park, an exclusively wildlife sanctuary in South Africa. These ticks transmitted Cowdria ruminantium, the rickettsial agent causing heartwater, to a susceptible goat, resulting in acute, fatal disease. C. ruminantium was isolated in bovine endothelial cell culture from the plasma of this animal during the febrile stage of the disease and transmitted to susceptible goats, causing fatal heartwater. The prevalence of C. ruminantium infection in 292 ticks was determined by polymerase chain reaction (PCR) analysis to be 1.7 percent (95 percent confidence interval 0.71 to 4.0 percent). A DNA proble analysis, which is less sensitive than PCR, detected infection in three of the five PCR positive ticks. The remaning infections were below the detection limit of the DNA probe, which is approximately 70,000 organisms. This is the first evidence that a vector-wildlife cycle of transmission of C. r uminantium can be maintained independently of domestic ruminants.
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spelling CGSpace299202024-03-03T19:19:44Z Cowdria ruminantium infection in ticks in the Kruger National Park Peter, T.F. Bryson, N.R. Perry, Brian D. O'Callaghan, C.J. Medley, G.F. Smith, G.E. Mlambo, G. Horak, I.G. Burridge, M.J. Mahan, S.M. metastigmata cowdria ruminantium amblyomma hebraeum goats disease transmission morbidity isolation techniques Adult Amblyomma hebraeum ticks, the principle vector of heartwater (cowdriosis) of domestic ruminants in southern Africa, were collected in pheromone traps placed in Kruger National Park, an exclusively wildlife sanctuary in South Africa. These ticks transmitted Cowdria ruminantium, the rickettsial agent causing heartwater, to a susceptible goat, resulting in acute, fatal disease. C. ruminantium was isolated in bovine endothelial cell culture from the plasma of this animal during the febrile stage of the disease and transmitted to susceptible goats, causing fatal heartwater. The prevalence of C. ruminantium infection in 292 ticks was determined by polymerase chain reaction (PCR) analysis to be 1.7 percent (95 percent confidence interval 0.71 to 4.0 percent). A DNA proble analysis, which is less sensitive than PCR, detected infection in three of the five PCR positive ticks. The remaning infections were below the detection limit of the DNA probe, which is approximately 70,000 organisms. This is the first evidence that a vector-wildlife cycle of transmission of C. r uminantium can be maintained independently of domestic ruminants. 1999-09 2013-06-11T09:25:26Z 2013-06-11T09:25:26Z Journal Article https://hdl.handle.net/10568/29920 en Limited Access Wiley Veterinary Record;145(11): 304-307
spellingShingle metastigmata
cowdria ruminantium
amblyomma hebraeum
goats
disease transmission
morbidity
isolation techniques
Peter, T.F.
Bryson, N.R.
Perry, Brian D.
O'Callaghan, C.J.
Medley, G.F.
Smith, G.E.
Mlambo, G.
Horak, I.G.
Burridge, M.J.
Mahan, S.M.
Cowdria ruminantium infection in ticks in the Kruger National Park
title Cowdria ruminantium infection in ticks in the Kruger National Park
title_full Cowdria ruminantium infection in ticks in the Kruger National Park
title_fullStr Cowdria ruminantium infection in ticks in the Kruger National Park
title_full_unstemmed Cowdria ruminantium infection in ticks in the Kruger National Park
title_short Cowdria ruminantium infection in ticks in the Kruger National Park
title_sort cowdria ruminantium infection in ticks in the kruger national park
topic metastigmata
cowdria ruminantium
amblyomma hebraeum
goats
disease transmission
morbidity
isolation techniques
url https://hdl.handle.net/10568/29920
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