Comparative sensitivity of antigen-detection enzyme immunosorbent assay and the microhaematocrit centrifugation technique in the diagnosis of Trypanosoma brucei infections in cattle
Four Boran cattle were infected with Trypanosoma brucei using Glossina morsitans centralis and were left untreated throughout the experimental period of 18 months. During this period, sequential blood samples were collected and examined for the presence of anti-trypanosome antibodies and their antig...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
1995
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/29911 |
| _version_ | 1855525088434061312 |
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| author | Masake, R.A. Moloo, S.K. Nantulya, V.M. Minja, S.H. Makau, J.M. Njuguna, J.T. |
| author_browse | Makau, J.M. Masake, R.A. Minja, S.H. Moloo, S.K. Nantulya, V.M. Njuguna, J.T. |
| author_facet | Masake, R.A. Moloo, S.K. Nantulya, V.M. Minja, S.H. Makau, J.M. Njuguna, J.T. |
| author_sort | Masake, R.A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Four Boran cattle were infected with Trypanosoma brucei using Glossina morsitans centralis and were left untreated throughout the experimental period of 18 months. During this period, sequential blood samples were collected and examined for the presence of anti-trypanosome antibodies and their antigens . Using the buffy coat technique (BCT), trypanosomes were detected in 38 (16.3 percent) of the 233 blood samples. Unlike the BCT, antigen-detection enzyme-linked immunosorbent assay (Ag-ELISA) diagnosed infections in 189 (81.1 percent) of the blood samples. These results were supported by the presence of antitrypanosome antibodies in the same samples. Thus Ag-ELISA was 5.5 times more sensitive than the BCT. Towards the end of the observation period, G.m. centralis tsetse were fed on the aparasitaemic cattle to determine whether they still hardboured the infection as the persistent antigenaemia seemed to suggest. Bloodmeals from the four cattle were infective to tsetse, thus emphasising the importance of Ag-ELISA in diagnosis of sub-patent infections. |
| format | Journal Article |
| id | CGSpace29911 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1995 |
| publishDateRange | 1995 |
| publishDateSort | 1995 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace299112024-05-01T08:17:20Z Comparative sensitivity of antigen-detection enzyme immunosorbent assay and the microhaematocrit centrifugation technique in the diagnosis of Trypanosoma brucei infections in cattle Masake, R.A. Moloo, S.K. Nantulya, V.M. Minja, S.H. Makau, J.M. Njuguna, J.T. cattle antigens trypanosoma brucei animal diseases infection diagnosis immunological techniques disease control Four Boran cattle were infected with Trypanosoma brucei using Glossina morsitans centralis and were left untreated throughout the experimental period of 18 months. During this period, sequential blood samples were collected and examined for the presence of anti-trypanosome antibodies and their antigens . Using the buffy coat technique (BCT), trypanosomes were detected in 38 (16.3 percent) of the 233 blood samples. Unlike the BCT, antigen-detection enzyme-linked immunosorbent assay (Ag-ELISA) diagnosed infections in 189 (81.1 percent) of the blood samples. These results were supported by the presence of antitrypanosome antibodies in the same samples. Thus Ag-ELISA was 5.5 times more sensitive than the BCT. Towards the end of the observation period, G.m. centralis tsetse were fed on the aparasitaemic cattle to determine whether they still hardboured the infection as the persistent antigenaemia seemed to suggest. Bloodmeals from the four cattle were infective to tsetse, thus emphasising the importance of Ag-ELISA in diagnosis of sub-patent infections. 1995-01 2013-06-11T09:25:23Z 2013-06-11T09:25:23Z Journal Article https://hdl.handle.net/10568/29911 en Limited Access Elsevier Veterinary Parasitology;56: 37-46 |
| spellingShingle | cattle antigens trypanosoma brucei animal diseases infection diagnosis immunological techniques disease control Masake, R.A. Moloo, S.K. Nantulya, V.M. Minja, S.H. Makau, J.M. Njuguna, J.T. Comparative sensitivity of antigen-detection enzyme immunosorbent assay and the microhaematocrit centrifugation technique in the diagnosis of Trypanosoma brucei infections in cattle |
| title | Comparative sensitivity of antigen-detection enzyme immunosorbent assay and the microhaematocrit centrifugation technique in the diagnosis of Trypanosoma brucei infections in cattle |
| title_full | Comparative sensitivity of antigen-detection enzyme immunosorbent assay and the microhaematocrit centrifugation technique in the diagnosis of Trypanosoma brucei infections in cattle |
| title_fullStr | Comparative sensitivity of antigen-detection enzyme immunosorbent assay and the microhaematocrit centrifugation technique in the diagnosis of Trypanosoma brucei infections in cattle |
| title_full_unstemmed | Comparative sensitivity of antigen-detection enzyme immunosorbent assay and the microhaematocrit centrifugation technique in the diagnosis of Trypanosoma brucei infections in cattle |
| title_short | Comparative sensitivity of antigen-detection enzyme immunosorbent assay and the microhaematocrit centrifugation technique in the diagnosis of Trypanosoma brucei infections in cattle |
| title_sort | comparative sensitivity of antigen detection enzyme immunosorbent assay and the microhaematocrit centrifugation technique in the diagnosis of trypanosoma brucei infections in cattle |
| topic | cattle antigens trypanosoma brucei animal diseases infection diagnosis immunological techniques disease control |
| url | https://hdl.handle.net/10568/29911 |
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