Bovine stem cell factor: Production of a biologically active protein and mRNA analysis in cattle infected with Trypanosoma congolense
The cDNA coding for the soluble form of bovine stem cell factor (boSCF-Ala-105) was cloned and recombinant protein was produced in bacteria as a histidine tagged-protein. The protein was purified from the inclusion bodies in one step by metal chelation chromatography under denaturing conditions. Rec...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Elsevier
1997
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/29869 |
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