Bovine CD4<sup(+)> T-cell lines reactive with soluble and membrane antigens of Cowdria ruminantium
Cowdria-specific CD4<sup(+)> T-cell lines generated from immunized cattle responded to both soluble and membrane proteins of the agent. The lines produced the Cowdria-inhibitory cytokine IFN-<gamma> in response to soluble antigens fractionated by gel filtration and fast performance liquid chromatogr...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
1999
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/29866 |
| _version_ | 1855514108861874176 |
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| author | Totte, P. Nyanjui, J. Bensaid, A. McKeever, Declan J. |
| author_browse | Bensaid, A. McKeever, Declan J. Nyanjui, J. Totte, P. |
| author_facet | Totte, P. Nyanjui, J. Bensaid, A. McKeever, Declan J. |
| author_sort | Totte, P. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Cowdria-specific CD4<sup(+)> T-cell lines generated from immunized cattle responded to both soluble and membrane proteins of the agent. The lines produced the Cowdria-inhibitory cytokine IFN-<gamma> in response to soluble antigens fractionated by gel filtration and fast performance liquid chromatography. Activity eluted as a single peak around fraction 15 for all T-cell lines tested. This fraction induced the highest production of IFN-<gamma> by the lines and was shown by SDS-PAGE and silver staining analysis to contain less than 10 different bands ranging from 22 to 32 kDa. It is suggested that given their high sensitivity and specificity, these short-term CD4<sup(+)> T-cell lines will be valuable tools for the identification of Cowdria antigens for incorporation in a subunit vaccine. |
| format | Journal Article |
| id | CGSpace29866 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1999 |
| publishDateRange | 1999 |
| publishDateSort | 1999 |
| record_format | dspace |
| spelling | CGSpace298662022-01-29T16:17:17Z Bovine CD4<sup(+)> T-cell lines reactive with soluble and membrane antigens of Cowdria ruminantium Totte, P. Nyanjui, J. Bensaid, A. McKeever, Declan J. antigens cowdria ruminantium t lymphocytes cell lines cattle cytokines cd4+ lymphocytes surface antigens Cowdria-specific CD4<sup(+)> T-cell lines generated from immunized cattle responded to both soluble and membrane proteins of the agent. The lines produced the Cowdria-inhibitory cytokine IFN-<gamma> in response to soluble antigens fractionated by gel filtration and fast performance liquid chromatography. Activity eluted as a single peak around fraction 15 for all T-cell lines tested. This fraction induced the highest production of IFN-<gamma> by the lines and was shown by SDS-PAGE and silver staining analysis to contain less than 10 different bands ranging from 22 to 32 kDa. It is suggested that given their high sensitivity and specificity, these short-term CD4<sup(+)> T-cell lines will be valuable tools for the identification of Cowdria antigens for incorporation in a subunit vaccine. 1999 2013-06-11T09:25:12Z 2013-06-11T09:25:12Z Journal Article https://hdl.handle.net/10568/29866 en Limited Access Veterinary Immunology and Immunopathology;70(3/4): 269-276 |
| spellingShingle | antigens cowdria ruminantium t lymphocytes cell lines cattle cytokines cd4+ lymphocytes surface antigens Totte, P. Nyanjui, J. Bensaid, A. McKeever, Declan J. Bovine CD4<sup(+)> T-cell lines reactive with soluble and membrane antigens of Cowdria ruminantium |
| title | Bovine CD4<sup(+)> T-cell lines reactive with soluble and membrane antigens of Cowdria ruminantium |
| title_full | Bovine CD4<sup(+)> T-cell lines reactive with soluble and membrane antigens of Cowdria ruminantium |
| title_fullStr | Bovine CD4<sup(+)> T-cell lines reactive with soluble and membrane antigens of Cowdria ruminantium |
| title_full_unstemmed | Bovine CD4<sup(+)> T-cell lines reactive with soluble and membrane antigens of Cowdria ruminantium |
| title_short | Bovine CD4<sup(+)> T-cell lines reactive with soluble and membrane antigens of Cowdria ruminantium |
| title_sort | bovine cd4 sup t cell lines reactive with soluble and membrane antigens of cowdria ruminantium |
| topic | antigens cowdria ruminantium t lymphocytes cell lines cattle cytokines cd4+ lymphocytes surface antigens |
| url | https://hdl.handle.net/10568/29866 |
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