Biochemical analysis of preliminary clusters in the non-lineage panel
After the preliminary clustering of the whole workshop panel using flowcytometry data, we selected those clusters that were composed of mAbs that bound a broad range of cells. To obtain evidence that mAbs in each of these preliminary clusters detected the same antigen, they were tested for their cap...
| Autores principales: | , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
1996
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/29859 |
| _version_ | 1855520349171482624 |
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| author | Naessens, Jan Nthale, J.M. Muiya, P. |
| author_browse | Muiya, P. Naessens, Jan Nthale, J.M. |
| author_facet | Naessens, Jan Nthale, J.M. Muiya, P. |
| author_sort | Naessens, Jan |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | After the preliminary clustering of the whole workshop panel using flowcytometry data, we selected those clusters that were composed of mAbs that bound a broad range of cells. To obtain evidence that mAbs in each of these preliminary clusters detected the same antigen, they were tested for their capacity to compete with each other for binding to a target cell and the molecular weights of their antigens were estimated after immunoprecipitation. Most preliminary clusters in the non-lineage panel contained control mAbs that had been characterised in one of the previous workshops, and this study therefore increased the number of mAbs available to each of these non-lineage antigens. One new interesting cluster, BoWC14, was described which defined an antigen on myeloid cells and a subpopulation of CD4, CD8 and WC1 T lymphocytes (BT3/8.12 and IL-A155). An additional cluster, which did not fit into any other panel, contained mAbs specific for an antigen restricted to the erythroid lineage and received the nomenclature BoWC15 (ANA8, IL-A135, IL-A137, IL-A138 and IL-A160). |
| format | Journal Article |
| id | CGSpace29859 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1996 |
| publishDateRange | 1996 |
| publishDateSort | 1996 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace298592023-12-08T19:36:04Z Biochemical analysis of preliminary clusters in the non-lineage panel Naessens, Jan Nthale, J.M. Muiya, P. biochemistry antibodies zebu cattle humpless cattle After the preliminary clustering of the whole workshop panel using flowcytometry data, we selected those clusters that were composed of mAbs that bound a broad range of cells. To obtain evidence that mAbs in each of these preliminary clusters detected the same antigen, they were tested for their capacity to compete with each other for binding to a target cell and the molecular weights of their antigens were estimated after immunoprecipitation. Most preliminary clusters in the non-lineage panel contained control mAbs that had been characterised in one of the previous workshops, and this study therefore increased the number of mAbs available to each of these non-lineage antigens. One new interesting cluster, BoWC14, was described which defined an antigen on myeloid cells and a subpopulation of CD4, CD8 and WC1 T lymphocytes (BT3/8.12 and IL-A155). An additional cluster, which did not fit into any other panel, contained mAbs specific for an antigen restricted to the erythroid lineage and received the nomenclature BoWC15 (ANA8, IL-A135, IL-A137, IL-A138 and IL-A160). 1996-08 2013-06-11T09:25:10Z 2013-06-11T09:25:10Z Journal Article https://hdl.handle.net/10568/29859 en Limited Access Elsevier Veterinary Immunology and Immunopathology;52(4): 347-356 |
| spellingShingle | biochemistry antibodies zebu cattle humpless cattle Naessens, Jan Nthale, J.M. Muiya, P. Biochemical analysis of preliminary clusters in the non-lineage panel |
| title | Biochemical analysis of preliminary clusters in the non-lineage panel |
| title_full | Biochemical analysis of preliminary clusters in the non-lineage panel |
| title_fullStr | Biochemical analysis of preliminary clusters in the non-lineage panel |
| title_full_unstemmed | Biochemical analysis of preliminary clusters in the non-lineage panel |
| title_short | Biochemical analysis of preliminary clusters in the non-lineage panel |
| title_sort | biochemical analysis of preliminary clusters in the non lineage panel |
| topic | biochemistry antibodies zebu cattle humpless cattle |
| url | https://hdl.handle.net/10568/29859 |
| work_keys_str_mv | AT naessensjan biochemicalanalysisofpreliminaryclustersinthenonlineagepanel AT nthalejm biochemicalanalysisofpreliminaryclustersinthenonlineagepanel AT muiyap biochemicalanalysisofpreliminaryclustersinthenonlineagepanel |