Analysis of monoclonal antibodies reactive with molecules upregulated or expressed only on activated lymphocytes
Monoclonal antibodies potentially specific for antigens expressed or upregulated on activated leukocytes were selected for further analysis from the panel submitted to the third international workshop on ruminant leukocyte antigens. The kinetics of expression of these activation antigens or resting...
| Autores principales: | , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
1996
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/29858 |
| _version_ | 1855527599595323392 |
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| author | Davis, W.C. Naessens, Jan Brown, W.C. Ellis, J.A. Hamilton, M.J. Canton, G.H. Barbosa, J.J.R. Ferens, W. Bohach, G.A. |
| author_browse | Barbosa, J.J.R. Bohach, G.A. Brown, W.C. Canton, G.H. Davis, W.C. Ellis, J.A. Ferens, W. Hamilton, M.J. Naessens, Jan |
| author_facet | Davis, W.C. Naessens, Jan Brown, W.C. Ellis, J.A. Hamilton, M.J. Canton, G.H. Barbosa, J.J.R. Ferens, W. Bohach, G.A. |
| author_sort | Davis, W.C. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Monoclonal antibodies potentially specific for antigens expressed or upregulated on activated leukocytes were selected for further analysis from the panel submitted to the third international workshop on ruminant leukocyte antigens. The kinetics of expression of these activation antigens or resting peripheral mononuclear cells (PBMC) and PBMC stimulated with concanavalin A or staphylococcal superantigen SEC1 for 4, 24 or 96 h were compared, as well as their appearance on various subsets of cells. For some of them, a molecular mass could be determined after immunoprecipitation from radio-labeled, lectin-stimulated cells. Based on the results from the clustering, kinetic studies and biochemical data, evidence was gathered for assigning two additional mAbs to cluster BoCD25 (IL-2 receptor) and two mAbs to cluster BoCD71 (transferrin receptor). Four mAbs recognized an early activation antigen predominantly expressed on alpha gamma T cells in short-term cultures. A number of other activation antigens were further characterized. |
| format | Journal Article |
| id | CGSpace29858 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1996 |
| publishDateRange | 1996 |
| publishDateSort | 1996 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace298582024-04-25T06:01:05Z Analysis of monoclonal antibodies reactive with molecules upregulated or expressed only on activated lymphocytes Davis, W.C. Naessens, Jan Brown, W.C. Ellis, J.A. Hamilton, M.J. Canton, G.H. Barbosa, J.J.R. Ferens, W. Bohach, G.A. monoclonal antibodies lymphocytes ruminants biochemistry Monoclonal antibodies potentially specific for antigens expressed or upregulated on activated leukocytes were selected for further analysis from the panel submitted to the third international workshop on ruminant leukocyte antigens. The kinetics of expression of these activation antigens or resting peripheral mononuclear cells (PBMC) and PBMC stimulated with concanavalin A or staphylococcal superantigen SEC1 for 4, 24 or 96 h were compared, as well as their appearance on various subsets of cells. For some of them, a molecular mass could be determined after immunoprecipitation from radio-labeled, lectin-stimulated cells. Based on the results from the clustering, kinetic studies and biochemical data, evidence was gathered for assigning two additional mAbs to cluster BoCD25 (IL-2 receptor) and two mAbs to cluster BoCD71 (transferrin receptor). Four mAbs recognized an early activation antigen predominantly expressed on alpha gamma T cells in short-term cultures. A number of other activation antigens were further characterized. 1996-08 2013-06-11T09:25:10Z 2013-06-11T09:25:10Z Journal Article https://hdl.handle.net/10568/29858 en Limited Access Elsevier Veterinary Immunology and Immunopathology;52(4): 301-311 |
| spellingShingle | monoclonal antibodies lymphocytes ruminants biochemistry Davis, W.C. Naessens, Jan Brown, W.C. Ellis, J.A. Hamilton, M.J. Canton, G.H. Barbosa, J.J.R. Ferens, W. Bohach, G.A. Analysis of monoclonal antibodies reactive with molecules upregulated or expressed only on activated lymphocytes |
| title | Analysis of monoclonal antibodies reactive with molecules upregulated or expressed only on activated lymphocytes |
| title_full | Analysis of monoclonal antibodies reactive with molecules upregulated or expressed only on activated lymphocytes |
| title_fullStr | Analysis of monoclonal antibodies reactive with molecules upregulated or expressed only on activated lymphocytes |
| title_full_unstemmed | Analysis of monoclonal antibodies reactive with molecules upregulated or expressed only on activated lymphocytes |
| title_short | Analysis of monoclonal antibodies reactive with molecules upregulated or expressed only on activated lymphocytes |
| title_sort | analysis of monoclonal antibodies reactive with molecules upregulated or expressed only on activated lymphocytes |
| topic | monoclonal antibodies lymphocytes ruminants biochemistry |
| url | https://hdl.handle.net/10568/29858 |
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