Delivery of the Theileria parva p67 antigen to cattle using recombinant vaccinia virus: IL-2 enhances protection
To evaluate vaccinia virus as a delivery system for recombinant antigen in cattle, calves were immunized with a recombinant vaccinia virus (rVV) expressing the sporozoite surface antigen (p67) of Theileria parva (V-67) combined with those expressing bovine IL-4 (V-IL4) or IL-2 (V-IL2). The anti-p67...
| Autores principales: | , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
1998
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/29820 |
| _version_ | 1855531613578854400 |
|---|---|
| author | Honda, Y. Waithaka, M.M. Taracha, E.L.N. Duchateau, L. Musoke, A.J. McKeever, Declan J. |
| author_browse | Duchateau, L. Honda, Y. McKeever, Declan J. Musoke, A.J. Taracha, E.L.N. Waithaka, M.M. |
| author_facet | Honda, Y. Waithaka, M.M. Taracha, E.L.N. Duchateau, L. Musoke, A.J. McKeever, Declan J. |
| author_sort | Honda, Y. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | To evaluate vaccinia virus as a delivery system for recombinant antigen in cattle, calves were immunized with a recombinant vaccinia virus (rVV) expressing the sporozoite surface antigen (p67) of Theileria parva (V-67) combined with those expressing bovine IL-4 (V-IL4) or IL-2 (V-IL2). The anti-p67 antibody levels detected in calves inoculated with the combination of V-67 and V-IL2. On challenge with cryopreserved sporozoites. 5 of 7 animals receiving V-67 combined with V-IL2 were protected, while those receiving V-67 in conjunction with V-IL4 behaved like unimmunized control calves. Vaccination with a recombinant virus expressing a chimaeric p67(p583)/IL2 product gave rise to a lower level of protection, whereas V-IL2 provided no immunity. The results of this study demonstrate the potential of rVV as a delivery system for use in vaccination of cattle against Theileria parva infection. |
| format | Journal Article |
| id | CGSpace29820 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1998 |
| publishDateRange | 1998 |
| publishDateSort | 1998 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace298202024-04-25T06:01:20Z Delivery of the Theileria parva p67 antigen to cattle using recombinant vaccinia virus: IL-2 enhances protection Honda, Y. Waithaka, M.M. Taracha, E.L.N. Duchateau, L. Musoke, A.J. McKeever, Declan J. theileria parva antigens cattle viruses cytokines To evaluate vaccinia virus as a delivery system for recombinant antigen in cattle, calves were immunized with a recombinant vaccinia virus (rVV) expressing the sporozoite surface antigen (p67) of Theileria parva (V-67) combined with those expressing bovine IL-4 (V-IL4) or IL-2 (V-IL2). The anti-p67 antibody levels detected in calves inoculated with the combination of V-67 and V-IL2. On challenge with cryopreserved sporozoites. 5 of 7 animals receiving V-67 combined with V-IL2 were protected, while those receiving V-67 in conjunction with V-IL4 behaved like unimmunized control calves. Vaccination with a recombinant virus expressing a chimaeric p67(p583)/IL2 product gave rise to a lower level of protection, whereas V-IL2 provided no immunity. The results of this study demonstrate the potential of rVV as a delivery system for use in vaccination of cattle against Theileria parva infection. 1998-08 2013-06-11T09:25:01Z 2013-06-11T09:25:01Z Journal Article https://hdl.handle.net/10568/29820 en Limited Access Elsevier Vaccine;16(13): 1276-1282 |
| spellingShingle | theileria parva antigens cattle viruses cytokines Honda, Y. Waithaka, M.M. Taracha, E.L.N. Duchateau, L. Musoke, A.J. McKeever, Declan J. Delivery of the Theileria parva p67 antigen to cattle using recombinant vaccinia virus: IL-2 enhances protection |
| title | Delivery of the Theileria parva p67 antigen to cattle using recombinant vaccinia virus: IL-2 enhances protection |
| title_full | Delivery of the Theileria parva p67 antigen to cattle using recombinant vaccinia virus: IL-2 enhances protection |
| title_fullStr | Delivery of the Theileria parva p67 antigen to cattle using recombinant vaccinia virus: IL-2 enhances protection |
| title_full_unstemmed | Delivery of the Theileria parva p67 antigen to cattle using recombinant vaccinia virus: IL-2 enhances protection |
| title_short | Delivery of the Theileria parva p67 antigen to cattle using recombinant vaccinia virus: IL-2 enhances protection |
| title_sort | delivery of the theileria parva p67 antigen to cattle using recombinant vaccinia virus il 2 enhances protection |
| topic | theileria parva antigens cattle viruses cytokines |
| url | https://hdl.handle.net/10568/29820 |
| work_keys_str_mv | AT honday deliveryofthetheileriaparvap67antigentocattleusingrecombinantvacciniavirusil2enhancesprotection AT waithakamm deliveryofthetheileriaparvap67antigentocattleusingrecombinantvacciniavirusil2enhancesprotection AT tarachaeln deliveryofthetheileriaparvap67antigentocattleusingrecombinantvacciniavirusil2enhancesprotection AT duchateaul deliveryofthetheileriaparvap67antigentocattleusingrecombinantvacciniavirusil2enhancesprotection AT musokeaj deliveryofthetheileriaparvap67antigentocattleusingrecombinantvacciniavirusil2enhancesprotection AT mckeeverdeclanj deliveryofthetheileriaparvap67antigentocattleusingrecombinantvacciniavirusil2enhancesprotection |