Characterisation of a potyvirus from Centrosema pubescens Benth
A virus, 750 /I > 18 nm, was isolated from Centrosema pubescens from Nigeria, causing leaf malformation, mosaic and mottling on the plant. When inoculated on to various test plants, it failed to produce necrotic or chlorotic lesions on Chenopodium amaranticolor, but symptoms of mottling were observe...
| Autores principales: | , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2007
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/29810 |
| _version_ | 1855528436685078528 |
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| author | Odedara, O.O. Hughes, Jacqueline d'Arros Tarawali, Shirley A. Odebode, A.C. Winter, S. |
| author_browse | Hughes, Jacqueline d'Arros Odebode, A.C. Odedara, O.O. Tarawali, Shirley A. Winter, S. |
| author_facet | Odedara, O.O. Hughes, Jacqueline d'Arros Tarawali, Shirley A. Odebode, A.C. Winter, S. |
| author_sort | Odedara, O.O. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | A virus, 750 /I > 18 nm, was isolated from Centrosema pubescens from Nigeria, causing leaf malformation, mosaic and mottling on the plant. When inoculated on to various test plants, it failed to produce necrotic or chlorotic lesions on Chenopodium amaranticolor, but symptoms of mottling were observed. The virus did not infect cowpea (Vigna unguiculata varieties IT82E-10, IT84S-2114, Ife brown, TVU 76 or TVU 2657), but produced chlorotic spots, vein clearing and mottling on soybean (Glycine max cultivars Malayan and Samsoy-2). Protein-A sandwich enzyme-linked immunosorbent assay (ELISA) shows that the virus is serologically closely related to Sweet potato feathery mottle virus (SPFMV) genus Potyvirus, Bean yellow mosaic virus (BYMV) genus Potyvirus and Tobacco etch virus (TEV) genus Potyvirus. Comparison of amino acid sequences of the coat protein of the virus with several potyvirus coat protein sequences, using BLAST (Basic Local Alignment Search Tool) analysis, confirmed the relationships. The antiserum raised against the virus had a titre value greater than 1 : 16,000, using antigen-coated plate ELISA. The name Centrosema mosaic virus (CenMoV) for this apparently new Potyvirus is suggested. The consequences of infection of forage legumes with this virus are discussed. |
| format | Journal Article |
| id | CGSpace29810 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2007 |
| publishDateRange | 2007 |
| publishDateSort | 2007 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace298102024-03-22T07:35:23Z Characterisation of a potyvirus from Centrosema pubescens Benth Odedara, O.O. Hughes, Jacqueline d'Arros Tarawali, Shirley A. Odebode, A.C. Winter, S. centrosema pubescens potyviruses plant viruses immunology centrosenxa pubescens mosaic potyvirus serology characterisation A virus, 750 /I > 18 nm, was isolated from Centrosema pubescens from Nigeria, causing leaf malformation, mosaic and mottling on the plant. When inoculated on to various test plants, it failed to produce necrotic or chlorotic lesions on Chenopodium amaranticolor, but symptoms of mottling were observed. The virus did not infect cowpea (Vigna unguiculata varieties IT82E-10, IT84S-2114, Ife brown, TVU 76 or TVU 2657), but produced chlorotic spots, vein clearing and mottling on soybean (Glycine max cultivars Malayan and Samsoy-2). Protein-A sandwich enzyme-linked immunosorbent assay (ELISA) shows that the virus is serologically closely related to Sweet potato feathery mottle virus (SPFMV) genus Potyvirus, Bean yellow mosaic virus (BYMV) genus Potyvirus and Tobacco etch virus (TEV) genus Potyvirus. Comparison of amino acid sequences of the coat protein of the virus with several potyvirus coat protein sequences, using BLAST (Basic Local Alignment Search Tool) analysis, confirmed the relationships. The antiserum raised against the virus had a titre value greater than 1 : 16,000, using antigen-coated plate ELISA. The name Centrosema mosaic virus (CenMoV) for this apparently new Potyvirus is suggested. The consequences of infection of forage legumes with this virus are discussed. 2007-03 2013-06-11T09:24:59Z 2013-06-11T09:24:59Z Journal Article https://hdl.handle.net/10568/29810 en Open Access Wiley Tropical Science;47(1): 3-15 |
| spellingShingle | centrosema pubescens potyviruses plant viruses immunology centrosenxa pubescens mosaic potyvirus serology characterisation Odedara, O.O. Hughes, Jacqueline d'Arros Tarawali, Shirley A. Odebode, A.C. Winter, S. Characterisation of a potyvirus from Centrosema pubescens Benth |
| title | Characterisation of a potyvirus from Centrosema pubescens Benth |
| title_full | Characterisation of a potyvirus from Centrosema pubescens Benth |
| title_fullStr | Characterisation of a potyvirus from Centrosema pubescens Benth |
| title_full_unstemmed | Characterisation of a potyvirus from Centrosema pubescens Benth |
| title_short | Characterisation of a potyvirus from Centrosema pubescens Benth |
| title_sort | characterisation of a potyvirus from centrosema pubescens benth |
| topic | centrosema pubescens potyviruses plant viruses immunology centrosenxa pubescens mosaic potyvirus serology characterisation |
| url | https://hdl.handle.net/10568/29810 |
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