Evaluation of recombinant sporozoite antigen SPAG-1 as a vaccine candidate against Theileria annulata by the use of different delivery systems
The major sporozoite surface antigen of Theileria annulata (SPAG-1) is a candidate for inclusion in a subunit vaccine. In this paper we summarize the results of 4 vaccination experiments using recombinant SPAG-1 expressed in different systems and presented in different adjuvants. The antigen has bee...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Wiley
1999
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/29793 |
| _version_ | 1855523824820289536 |
|---|---|
| author | Boulter, N. Brown, D. Wilkie, G. Williamson, S. Kirvar, E. Knight, P. Glass, E. Campbell, J. Morzaria, S.P. Nene, Vishvanath M. Musoke, A.J. Oliveria, C. de Gubbels, M.J. Jongejan, F. Hall, R. |
| author_browse | Boulter, N. Brown, D. Campbell, J. Glass, E. Gubbels, M.J. Hall, R. Jongejan, F. Kirvar, E. Knight, P. Morzaria, S.P. Musoke, A.J. Nene, Vishvanath M. Oliveria, C. de Wilkie, G. Williamson, S. |
| author_facet | Boulter, N. Brown, D. Wilkie, G. Williamson, S. Kirvar, E. Knight, P. Glass, E. Campbell, J. Morzaria, S.P. Nene, Vishvanath M. Musoke, A.J. Oliveria, C. de Gubbels, M.J. Jongejan, F. Hall, R. |
| author_sort | Boulter, N. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The major sporozoite surface antigen of Theileria annulata (SPAG-1) is a candidate for inclusion in a subunit vaccine. In this paper we summarize the results of 4 vaccination experiments using recombinant SPAG-1 expressed in different systems and presented in different adjuvants. The antigen has been presented as either a C terminal 108 amino acid peptide (called SR1) expressed as both beta-galactosidase and hepatitis B core antigen fusions or as a full-length form expressed as a GST fusion with an N terminal His6 tag. Different adjuvants, namely Freund's, saponin, ISCOMs and a proprietary adjuvant supplied by SmithKline Beecham were used, which are called SKBA. The data point to the conclusion that SPAG-1 can elicit partial protection and is therefore suitable for inclusion in an eventual multicomponent subunit vaccine. |
| format | Journal Article |
| id | CGSpace29793 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1999 |
| publishDateRange | 1999 |
| publishDateSort | 1999 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace297932024-01-17T12:58:34Z Evaluation of recombinant sporozoite antigen SPAG-1 as a vaccine candidate against Theileria annulata by the use of different delivery systems Boulter, N. Brown, D. Wilkie, G. Williamson, S. Kirvar, E. Knight, P. Glass, E. Campbell, J. Morzaria, S.P. Nene, Vishvanath M. Musoke, A.J. Oliveria, C. de Gubbels, M.J. Jongejan, F. Hall, R. theileria parva vaccines antigens sporozoites immunization calves The major sporozoite surface antigen of Theileria annulata (SPAG-1) is a candidate for inclusion in a subunit vaccine. In this paper we summarize the results of 4 vaccination experiments using recombinant SPAG-1 expressed in different systems and presented in different adjuvants. The antigen has been presented as either a C terminal 108 amino acid peptide (called SR1) expressed as both beta-galactosidase and hepatitis B core antigen fusions or as a full-length form expressed as a GST fusion with an N terminal His6 tag. Different adjuvants, namely Freund's, saponin, ISCOMs and a proprietary adjuvant supplied by SmithKline Beecham were used, which are called SKBA. The data point to the conclusion that SPAG-1 can elicit partial protection and is therefore suitable for inclusion in an eventual multicomponent subunit vaccine. 1999-09 2013-06-11T09:24:55Z 2013-06-11T09:24:55Z Journal Article https://hdl.handle.net/10568/29793 en Limited Access Wiley Tropical Medicine & International Health;4(9): A71-A77 |
| spellingShingle | theileria parva vaccines antigens sporozoites immunization calves Boulter, N. Brown, D. Wilkie, G. Williamson, S. Kirvar, E. Knight, P. Glass, E. Campbell, J. Morzaria, S.P. Nene, Vishvanath M. Musoke, A.J. Oliveria, C. de Gubbels, M.J. Jongejan, F. Hall, R. Evaluation of recombinant sporozoite antigen SPAG-1 as a vaccine candidate against Theileria annulata by the use of different delivery systems |
| title | Evaluation of recombinant sporozoite antigen SPAG-1 as a vaccine candidate against Theileria annulata by the use of different delivery systems |
| title_full | Evaluation of recombinant sporozoite antigen SPAG-1 as a vaccine candidate against Theileria annulata by the use of different delivery systems |
| title_fullStr | Evaluation of recombinant sporozoite antigen SPAG-1 as a vaccine candidate against Theileria annulata by the use of different delivery systems |
| title_full_unstemmed | Evaluation of recombinant sporozoite antigen SPAG-1 as a vaccine candidate against Theileria annulata by the use of different delivery systems |
| title_short | Evaluation of recombinant sporozoite antigen SPAG-1 as a vaccine candidate against Theileria annulata by the use of different delivery systems |
| title_sort | evaluation of recombinant sporozoite antigen spag 1 as a vaccine candidate against theileria annulata by the use of different delivery systems |
| topic | theileria parva vaccines antigens sporozoites immunization calves |
| url | https://hdl.handle.net/10568/29793 |
| work_keys_str_mv | AT boultern evaluationofrecombinantsporozoiteantigenspag1asavaccinecandidateagainsttheileriaannulatabytheuseofdifferentdeliverysystems AT brownd evaluationofrecombinantsporozoiteantigenspag1asavaccinecandidateagainsttheileriaannulatabytheuseofdifferentdeliverysystems AT wilkieg evaluationofrecombinantsporozoiteantigenspag1asavaccinecandidateagainsttheileriaannulatabytheuseofdifferentdeliverysystems AT williamsons evaluationofrecombinantsporozoiteantigenspag1asavaccinecandidateagainsttheileriaannulatabytheuseofdifferentdeliverysystems AT kirvare evaluationofrecombinantsporozoiteantigenspag1asavaccinecandidateagainsttheileriaannulatabytheuseofdifferentdeliverysystems AT knightp evaluationofrecombinantsporozoiteantigenspag1asavaccinecandidateagainsttheileriaannulatabytheuseofdifferentdeliverysystems AT glasse evaluationofrecombinantsporozoiteantigenspag1asavaccinecandidateagainsttheileriaannulatabytheuseofdifferentdeliverysystems AT campbellj evaluationofrecombinantsporozoiteantigenspag1asavaccinecandidateagainsttheileriaannulatabytheuseofdifferentdeliverysystems AT morzariasp evaluationofrecombinantsporozoiteantigenspag1asavaccinecandidateagainsttheileriaannulatabytheuseofdifferentdeliverysystems AT nenevishvanathm evaluationofrecombinantsporozoiteantigenspag1asavaccinecandidateagainsttheileriaannulatabytheuseofdifferentdeliverysystems AT musokeaj evaluationofrecombinantsporozoiteantigenspag1asavaccinecandidateagainsttheileriaannulatabytheuseofdifferentdeliverysystems AT oliveriacde evaluationofrecombinantsporozoiteantigenspag1asavaccinecandidateagainsttheileriaannulatabytheuseofdifferentdeliverysystems AT gubbelsmj evaluationofrecombinantsporozoiteantigenspag1asavaccinecandidateagainsttheileriaannulatabytheuseofdifferentdeliverysystems AT jongejanf evaluationofrecombinantsporozoiteantigenspag1asavaccinecandidateagainsttheileriaannulatabytheuseofdifferentdeliverysystems AT hallr evaluationofrecombinantsporozoiteantigenspag1asavaccinecandidateagainsttheileriaannulatabytheuseofdifferentdeliverysystems |