Trapping parasite secretory proteins in baker's yeast
Because the function of signal sequences has been conserved during evolution it has been possible to develop both bioinformatics resources to identify them and techniques to clone genes that encode secretary proteins. The latter entail insertion of heterologous signals upstream of signal peptide del...
| Main Authors: | , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Elsevier
2001
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/29685 |
| _version_ | 1855520328511389696 |
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| author | Nene, Vishvanath M. Bishop, Richard P. |
| author_browse | Bishop, Richard P. Nene, Vishvanath M. |
| author_facet | Nene, Vishvanath M. Bishop, Richard P. |
| author_sort | Nene, Vishvanath M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Because the function of signal sequences has been conserved during evolution it has been possible to develop both bioinformatics resources to identify them and techniques to clone genes that encode secretary proteins. The latter entail insertion of heterologous signals upstream of signal peptide deleted reporter genes. We discuss the advantages of using Saccharomyces cerevisiae for signal sequence trap technology. The yeast protein-translocation system appears to be less discriminating than that of higher eukaryotes - for example, a Theileria parva cysteine protease gene containing a recessed, nonclassical signal allows access to the secretary pathway- and yeast technology could have general application in studying elements of parasite protein trafficking. |
| format | Journal Article |
| id | CGSpace29685 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2001 |
| publishDateRange | 2001 |
| publishDateSort | 2001 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace296852024-04-25T06:00:37Z Trapping parasite secretory proteins in baker's yeast Nene, Vishvanath M. Bishop, Richard P. parasites trapping yeast theileria parva proteins Because the function of signal sequences has been conserved during evolution it has been possible to develop both bioinformatics resources to identify them and techniques to clone genes that encode secretary proteins. The latter entail insertion of heterologous signals upstream of signal peptide deleted reporter genes. We discuss the advantages of using Saccharomyces cerevisiae for signal sequence trap technology. The yeast protein-translocation system appears to be less discriminating than that of higher eukaryotes - for example, a Theileria parva cysteine protease gene containing a recessed, nonclassical signal allows access to the secretary pathway- and yeast technology could have general application in studying elements of parasite protein trafficking. 2001-09 2013-06-11T09:24:29Z 2013-06-11T09:24:29Z Journal Article https://hdl.handle.net/10568/29685 en Limited Access Elsevier Trends in Parasitology;17(9): 407-409 |
| spellingShingle | parasites trapping yeast theileria parva proteins Nene, Vishvanath M. Bishop, Richard P. Trapping parasite secretory proteins in baker's yeast |
| title | Trapping parasite secretory proteins in baker's yeast |
| title_full | Trapping parasite secretory proteins in baker's yeast |
| title_fullStr | Trapping parasite secretory proteins in baker's yeast |
| title_full_unstemmed | Trapping parasite secretory proteins in baker's yeast |
| title_short | Trapping parasite secretory proteins in baker's yeast |
| title_sort | trapping parasite secretory proteins in baker s yeast |
| topic | parasites trapping yeast theileria parva proteins |
| url | https://hdl.handle.net/10568/29685 |
| work_keys_str_mv | AT nenevishvanathm trappingparasitesecretoryproteinsinbakersyeast AT bishoprichardp trappingparasitesecretoryproteinsinbakersyeast |