Gene-expression profiling discriminates between cerebral malaria (CM) - susceptible mice and CM-resistant mice
The development of cerebral malaria (CM) in mice with Plasmodium berghei ANKA infection is under genetic control. Brain gene-expression patterns were investigated in well-defined genetically CM-resistant (CM-R; BALB/c and DBA/2) and CM-susceptible (CM-S; C57BL/6 and CBA/J) mice by use of cDNA microa...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Oxford University Press
2006
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/29649 |
| _version_ | 1855530674217287680 |
|---|---|
| author | Delhaye, N.F. Coltel, N. Puthier, D. Flori, L. Houlgatte, R. Iraqi, F.A. Nguyen, C. Grau, G.E. Rihet, P. |
| author_browse | Coltel, N. Delhaye, N.F. Flori, L. Grau, G.E. Houlgatte, R. Iraqi, F.A. Nguyen, C. Puthier, D. Rihet, P. |
| author_facet | Delhaye, N.F. Coltel, N. Puthier, D. Flori, L. Houlgatte, R. Iraqi, F.A. Nguyen, C. Grau, G.E. Rihet, P. |
| author_sort | Delhaye, N.F. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The development of cerebral malaria (CM) in mice with Plasmodium berghei ANKA infection is under genetic control. Brain gene-expression patterns were investigated in well-defined genetically CM-resistant (CM-R; BALB/c and DBA/2) and CM-susceptible (CM-S; C57BL/6 and CBA/J) mice by use of cDNA microarrays. By combining transcriptional profiling with rigorous statistical methods and cluster analysis, we identified a set of 69 genes that perfectly discriminated between mouse strains and between CM-R and CM-S mice. The analysis of gene ontological terms revealed that the genes that clustered and were related to susceptibility to CM preferentially belonged to some biological process classes, such as those pertaining to immune responses. Using a false discovery rate of 5% and the Welch t test, we identified 31 genes with consistent differential expression between CM-R and CM-S mice. These data indicate that microarray analysis may be useful for identification of candidate genes that are potentially responsible for resistance or susceptibility to mouse CM and suggest that candidate genes identified in mice could be specifically tested in humans for an association with disease severity. |
| format | Journal Article |
| id | CGSpace29649 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2006 |
| publishDateRange | 2006 |
| publishDateSort | 2006 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | CGSpace296492023-12-08T19:36:04Z Gene-expression profiling discriminates between cerebral malaria (CM) - susceptible mice and CM-resistant mice Delhaye, N.F. Coltel, N. Puthier, D. Flori, L. Houlgatte, R. Iraqi, F.A. Nguyen, C. Grau, G.E. Rihet, P. malaria gene expression brain mice disease resistance immunity plasmodium berghei genetic control dna The development of cerebral malaria (CM) in mice with Plasmodium berghei ANKA infection is under genetic control. Brain gene-expression patterns were investigated in well-defined genetically CM-resistant (CM-R; BALB/c and DBA/2) and CM-susceptible (CM-S; C57BL/6 and CBA/J) mice by use of cDNA microarrays. By combining transcriptional profiling with rigorous statistical methods and cluster analysis, we identified a set of 69 genes that perfectly discriminated between mouse strains and between CM-R and CM-S mice. The analysis of gene ontological terms revealed that the genes that clustered and were related to susceptibility to CM preferentially belonged to some biological process classes, such as those pertaining to immune responses. Using a false discovery rate of 5% and the Welch t test, we identified 31 genes with consistent differential expression between CM-R and CM-S mice. These data indicate that microarray analysis may be useful for identification of candidate genes that are potentially responsible for resistance or susceptibility to mouse CM and suggest that candidate genes identified in mice could be specifically tested in humans for an association with disease severity. 2006-01-15 2013-06-11T09:24:20Z 2013-06-11T09:24:20Z Journal Article https://hdl.handle.net/10568/29649 en Limited Access Oxford University Press The Journal of Infectious Diseases;193(2): 312-321 |
| spellingShingle | malaria gene expression brain mice disease resistance immunity plasmodium berghei genetic control dna Delhaye, N.F. Coltel, N. Puthier, D. Flori, L. Houlgatte, R. Iraqi, F.A. Nguyen, C. Grau, G.E. Rihet, P. Gene-expression profiling discriminates between cerebral malaria (CM) - susceptible mice and CM-resistant mice |
| title | Gene-expression profiling discriminates between cerebral malaria (CM) - susceptible mice and CM-resistant mice |
| title_full | Gene-expression profiling discriminates between cerebral malaria (CM) - susceptible mice and CM-resistant mice |
| title_fullStr | Gene-expression profiling discriminates between cerebral malaria (CM) - susceptible mice and CM-resistant mice |
| title_full_unstemmed | Gene-expression profiling discriminates between cerebral malaria (CM) - susceptible mice and CM-resistant mice |
| title_short | Gene-expression profiling discriminates between cerebral malaria (CM) - susceptible mice and CM-resistant mice |
| title_sort | gene expression profiling discriminates between cerebral malaria cm susceptible mice and cm resistant mice |
| topic | malaria gene expression brain mice disease resistance immunity plasmodium berghei genetic control dna |
| url | https://hdl.handle.net/10568/29649 |
| work_keys_str_mv | AT delhayenf geneexpressionprofilingdiscriminatesbetweencerebralmalariacmsusceptiblemiceandcmresistantmice AT colteln geneexpressionprofilingdiscriminatesbetweencerebralmalariacmsusceptiblemiceandcmresistantmice AT puthierd geneexpressionprofilingdiscriminatesbetweencerebralmalariacmsusceptiblemiceandcmresistantmice AT floril geneexpressionprofilingdiscriminatesbetweencerebralmalariacmsusceptiblemiceandcmresistantmice AT houlgatter geneexpressionprofilingdiscriminatesbetweencerebralmalariacmsusceptiblemiceandcmresistantmice AT iraqifa geneexpressionprofilingdiscriminatesbetweencerebralmalariacmsusceptiblemiceandcmresistantmice AT nguyenc geneexpressionprofilingdiscriminatesbetweencerebralmalariacmsusceptiblemiceandcmresistantmice AT grauge geneexpressionprofilingdiscriminatesbetweencerebralmalariacmsusceptiblemiceandcmresistantmice AT rihetp geneexpressionprofilingdiscriminatesbetweencerebralmalariacmsusceptiblemiceandcmresistantmice |