Detection of seedborne fungi in Stylosanthes guianensis, S. hamata, and S. scabra

The effects of pretreatment with sodium hypochlorite and plating onto potato dextrose agar, oatmeal agar, water agar and blotter of the range and incidence of fungi isolated from seeds of Stylosanthes species were studied. A survey was made of the fungi associated with 39 seed lots of S. guianensis,...

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Main Authors: Nan, Z.B., Hanson, Jean
Format: Journal Article
Language:Inglés
Published: 1998
Subjects:
Online Access:https://hdl.handle.net/10568/29606
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author Nan, Z.B.
Hanson, Jean
author_browse Hanson, Jean
Nan, Z.B.
author_facet Nan, Z.B.
Hanson, Jean
author_sort Nan, Z.B.
collection Repository of Agricultural Research Outputs (CGSpace)
description The effects of pretreatment with sodium hypochlorite and plating onto potato dextrose agar, oatmeal agar, water agar and blotter of the range and incidence of fungi isolated from seeds of Stylosanthes species were studied. A survey was made of the fungi associated with 39 seed lots of S. guianensis, 25 lots of S. hamata, and 14 lots of S. scabra. Oatmeal agar with seed pretreatment was the most suitable method. The most frequently isolated fungi were Phoma glomerata, Rhizoctonia solani, Fusarium oxysporum, Alternaria alternata, Cladosporium cladosporioides and Penicillium species. Variation in incidence and species of seedborne fungi was observed among the three Stylosanthes species tested. Hard seed of S. hamata had a much lower incidence of seedborne fungi than other seed. The majority of fungi were isolated from the seed coat. Only a small percentage of infection was found in the seed endosperm. The pathogenicity test showed that all 78 isolates of 27 fungal species tested reduced the length of seedlings growing on water agar surface; where as Colletotrichum truncatum, C. gloeosporioides, Fusarium oxysporum, F. avenaceum, Botrytis cinerea, Rhizoctonia salani and F. acuminatum killed most of the test seedlings.
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spelling CGSpace296062022-01-29T16:14:38Z Detection of seedborne fungi in Stylosanthes guianensis, S. hamata, and S. scabra Nan, Z.B. Hanson, Jean seed borne organisms fungi stylosanthes guianensis stylosanthes hamata stylosanthes scabra weight germination isolation The effects of pretreatment with sodium hypochlorite and plating onto potato dextrose agar, oatmeal agar, water agar and blotter of the range and incidence of fungi isolated from seeds of Stylosanthes species were studied. A survey was made of the fungi associated with 39 seed lots of S. guianensis, 25 lots of S. hamata, and 14 lots of S. scabra. Oatmeal agar with seed pretreatment was the most suitable method. The most frequently isolated fungi were Phoma glomerata, Rhizoctonia solani, Fusarium oxysporum, Alternaria alternata, Cladosporium cladosporioides and Penicillium species. Variation in incidence and species of seedborne fungi was observed among the three Stylosanthes species tested. Hard seed of S. hamata had a much lower incidence of seedborne fungi than other seed. The majority of fungi were isolated from the seed coat. Only a small percentage of infection was found in the seed endosperm. The pathogenicity test showed that all 78 isolates of 27 fungal species tested reduced the length of seedlings growing on water agar surface; where as Colletotrichum truncatum, C. gloeosporioides, Fusarium oxysporum, F. avenaceum, Botrytis cinerea, Rhizoctonia salani and F. acuminatum killed most of the test seedlings. 1998 2013-06-11T09:24:10Z 2013-06-11T09:24:10Z Journal Article https://hdl.handle.net/10568/29606 en Limited Access Seed Science and Technology;26(2): 333-345
spellingShingle seed borne organisms
fungi
stylosanthes guianensis
stylosanthes hamata
stylosanthes scabra
weight
germination
isolation
Nan, Z.B.
Hanson, Jean
Detection of seedborne fungi in Stylosanthes guianensis, S. hamata, and S. scabra
title Detection of seedborne fungi in Stylosanthes guianensis, S. hamata, and S. scabra
title_full Detection of seedborne fungi in Stylosanthes guianensis, S. hamata, and S. scabra
title_fullStr Detection of seedborne fungi in Stylosanthes guianensis, S. hamata, and S. scabra
title_full_unstemmed Detection of seedborne fungi in Stylosanthes guianensis, S. hamata, and S. scabra
title_short Detection of seedborne fungi in Stylosanthes guianensis, S. hamata, and S. scabra
title_sort detection of seedborne fungi in stylosanthes guianensis s hamata and s scabra
topic seed borne organisms
fungi
stylosanthes guianensis
stylosanthes hamata
stylosanthes scabra
weight
germination
isolation
url https://hdl.handle.net/10568/29606
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