Restriction mapping of the genome of the protozoan parasite Theileria parva
We have used a modified linking clone strategy and pulsed-field gel electrophoresis to derive a map of the 29 Sfi I and 4 Not I sites in the 10 million base pair genome of the protozoan parasite Theileria parva. This was achieved in the absence of classical genetic information. The map reveals four...
| Autores principales: | , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
National Academy of Sciences
1992
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/29542 |
| _version_ | 1855537788615655424 |
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| author | Morzaria, S.P. Young, J.R. |
| author_browse | Morzaria, S.P. Young, J.R. |
| author_facet | Morzaria, S.P. Young, J.R. |
| author_sort | Morzaria, S.P. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | We have used a modified linking clone strategy and pulsed-field gel electrophoresis to derive a map of the 29 Sfi I and 4 Not I sites in the 10 million base pair genome of the protozoan parasite Theileria parva. This was achieved in the absence of classical genetic information. The map reveals four chromosomes. Several genes, including those for parasite antigens, have been located on the map, as has the single locus carrying the major repetitive sequence in this organism. The map forms the basis for a study of sexual recombination in T. parva, which will be of importance in the application of present methods of immunization. Aspects of the mapping strategy may be useful in the study of other small eukaryotic genomes. |
| format | Journal Article |
| id | CGSpace29542 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1992 |
| publishDateRange | 1992 |
| publishDateSort | 1992 |
| publisher | National Academy of Sciences |
| publisherStr | National Academy of Sciences |
| record_format | dspace |
| spelling | CGSpace295422024-10-17T09:47:59Z Restriction mapping of the genome of the protozoan parasite Theileria parva Morzaria, S.P. Young, J.R. parasites theileria parva genomes We have used a modified linking clone strategy and pulsed-field gel electrophoresis to derive a map of the 29 Sfi I and 4 Not I sites in the 10 million base pair genome of the protozoan parasite Theileria parva. This was achieved in the absence of classical genetic information. The map reveals four chromosomes. Several genes, including those for parasite antigens, have been located on the map, as has the single locus carrying the major repetitive sequence in this organism. The map forms the basis for a study of sexual recombination in T. parva, which will be of importance in the application of present methods of immunization. Aspects of the mapping strategy may be useful in the study of other small eukaryotic genomes. 1992-06-15 2013-06-11T09:23:56Z 2013-06-11T09:23:56Z Journal Article https://hdl.handle.net/10568/29542 en Open Access National Academy of Sciences Proceedings of the National Academy of Sciences of the USA;89: 5241-5245 |
| spellingShingle | parasites theileria parva genomes Morzaria, S.P. Young, J.R. Restriction mapping of the genome of the protozoan parasite Theileria parva |
| title | Restriction mapping of the genome of the protozoan parasite Theileria parva |
| title_full | Restriction mapping of the genome of the protozoan parasite Theileria parva |
| title_fullStr | Restriction mapping of the genome of the protozoan parasite Theileria parva |
| title_full_unstemmed | Restriction mapping of the genome of the protozoan parasite Theileria parva |
| title_short | Restriction mapping of the genome of the protozoan parasite Theileria parva |
| title_sort | restriction mapping of the genome of the protozoan parasite theileria parva |
| topic | parasites theileria parva genomes |
| url | https://hdl.handle.net/10568/29542 |
| work_keys_str_mv | AT morzariasp restrictionmappingofthegenomeoftheprotozoanparasitetheileriaparva AT youngjr restrictionmappingofthegenomeoftheprotozoanparasitetheileriaparva |