Standardization of molecular genetic techniques for the characterization of germplasm collections: The case of random amplified polymorphic DNA (RAPD)
Random amplified polymorphic DNA (RAPD) variation is being used increasingly to characterize germplasm collections. However, the technique has limitations due to the lack of reproducibility of results under certain conditions. Here we review some of the literature on the factors that are mainly resp...
| Main Authors: | , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
1996
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/29494 |
| _version_ | 1855518677310373888 |
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| author | Lowe, A.J. Hanotte, Olivier H. Guarino, Luigi |
| author_browse | Guarino, Luigi Hanotte, Olivier H. Lowe, A.J. |
| author_facet | Lowe, A.J. Hanotte, Olivier H. Guarino, Luigi |
| author_sort | Lowe, A.J. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Random amplified polymorphic DNA (RAPD) variation is being used increasingly to characterize germplasm collections. However, the technique has limitations due to the lack of reproducibility of results under certain conditions. Here we review some of the literature on the factors that are mainly responsible for variation in the generation of RAPD fragments, and examine ways to reduce these sources of unwanted variation. We propose a table containing a collation of all the descriptors thought necessary to describe the conditions and assessment procedures necessary for reproducible RAPD profiling of plant material. The standardization of procedure and presentation of results should facilitate the comparison of results both between and within laboratories working on similar material, and allow RAPDs to be used as a standard germplasm descriptor. |
| format | Journal Article |
| id | CGSpace29494 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1996 |
| publishDateRange | 1996 |
| publishDateSort | 1996 |
| record_format | dspace |
| spelling | CGSpace294942022-01-29T16:04:28Z Standardization of molecular genetic techniques for the characterization of germplasm collections: The case of random amplified polymorphic DNA (RAPD) Lowe, A.J. Hanotte, Olivier H. Guarino, Luigi germplasm dna molecular genetics genetic polymorphism Random amplified polymorphic DNA (RAPD) variation is being used increasingly to characterize germplasm collections. However, the technique has limitations due to the lack of reproducibility of results under certain conditions. Here we review some of the literature on the factors that are mainly responsible for variation in the generation of RAPD fragments, and examine ways to reduce these sources of unwanted variation. We propose a table containing a collation of all the descriptors thought necessary to describe the conditions and assessment procedures necessary for reproducible RAPD profiling of plant material. The standardization of procedure and presentation of results should facilitate the comparison of results both between and within laboratories working on similar material, and allow RAPDs to be used as a standard germplasm descriptor. 1996 2013-06-11T09:23:46Z 2013-06-11T09:23:46Z Journal Article https://hdl.handle.net/10568/29494 en Limited Access Plant Genetic Resources Newsletter;no. 107: 50-54 |
| spellingShingle | germplasm dna molecular genetics genetic polymorphism Lowe, A.J. Hanotte, Olivier H. Guarino, Luigi Standardization of molecular genetic techniques for the characterization of germplasm collections: The case of random amplified polymorphic DNA (RAPD) |
| title | Standardization of molecular genetic techniques for the characterization of germplasm collections: The case of random amplified polymorphic DNA (RAPD) |
| title_full | Standardization of molecular genetic techniques for the characterization of germplasm collections: The case of random amplified polymorphic DNA (RAPD) |
| title_fullStr | Standardization of molecular genetic techniques for the characterization of germplasm collections: The case of random amplified polymorphic DNA (RAPD) |
| title_full_unstemmed | Standardization of molecular genetic techniques for the characterization of germplasm collections: The case of random amplified polymorphic DNA (RAPD) |
| title_short | Standardization of molecular genetic techniques for the characterization of germplasm collections: The case of random amplified polymorphic DNA (RAPD) |
| title_sort | standardization of molecular genetic techniques for the characterization of germplasm collections the case of random amplified polymorphic dna rapd |
| topic | germplasm dna molecular genetics genetic polymorphism |
| url | https://hdl.handle.net/10568/29494 |
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