Genetic fingerprinting of Theileria parva using a telomeric DNA probe
A cloned Theileria parva telomeric DNA sequence, designated pTpUtel, was used to characterize T. parva stocks and clones by hybridization to EcoRI-digested DNA. Eight of the nine T. parva stocks tested were discriminated by the telomeric restriction-fragment-length polymorphisms (RFLOs). Two isolate...
| Main Authors: | , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
1996
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/29473 |
| _version_ | 1855522760196882432 |
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| author | Bishop, Richard P. Spooner, P.R. Sohanpal, B.K. |
| author_browse | Bishop, Richard P. Sohanpal, B.K. Spooner, P.R. |
| author_facet | Bishop, Richard P. Spooner, P.R. Sohanpal, B.K. |
| author_sort | Bishop, Richard P. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | A cloned Theileria parva telomeric DNA sequence, designated pTpUtel, was used to characterize T. parva stocks and clones by hybridization to EcoRI-digested DNA. Eight of the nine T. parva stocks tested were discriminated by the telomeric restriction-fragment-length polymorphisms (RFLOs). Two isolates derived from buffalo 7014 by tick feeding on different occasions were also differentiated using the probe. The probe gave comparable results on purified piroplasm or schizont-infected lymphocyte DNA and did not cross-hybridize with uninfected bovine DNA. The telomeric restriction pattern of a cloned T. parva parasite remained identical after four passages through ticks and cattle. The telomeric sequence therefore represents a useful additional tool for analysis of theileriosis epidemiology. |
| format | Journal Article |
| id | CGSpace29473 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1996 |
| publishDateRange | 1996 |
| publishDateSort | 1996 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace294732024-05-01T08:16:47Z Genetic fingerprinting of Theileria parva using a telomeric DNA probe Bishop, Richard P. Spooner, P.R. Sohanpal, B.K. dna fingerprinting theileria parva genetics A cloned Theileria parva telomeric DNA sequence, designated pTpUtel, was used to characterize T. parva stocks and clones by hybridization to EcoRI-digested DNA. Eight of the nine T. parva stocks tested were discriminated by the telomeric restriction-fragment-length polymorphisms (RFLOs). Two isolates derived from buffalo 7014 by tick feeding on different occasions were also differentiated using the probe. The probe gave comparable results on purified piroplasm or schizont-infected lymphocyte DNA and did not cross-hybridize with uninfected bovine DNA. The telomeric restriction pattern of a cloned T. parva parasite remained identical after four passages through ticks and cattle. The telomeric sequence therefore represents a useful additional tool for analysis of theileriosis epidemiology. 1996-02-29 2013-06-11T09:23:41Z 2013-06-11T09:23:41Z Journal Article https://hdl.handle.net/10568/29473 en Limited Access Springer Parasitology Research;82: 275-280 |
| spellingShingle | dna fingerprinting theileria parva genetics Bishop, Richard P. Spooner, P.R. Sohanpal, B.K. Genetic fingerprinting of Theileria parva using a telomeric DNA probe |
| title | Genetic fingerprinting of Theileria parva using a telomeric DNA probe |
| title_full | Genetic fingerprinting of Theileria parva using a telomeric DNA probe |
| title_fullStr | Genetic fingerprinting of Theileria parva using a telomeric DNA probe |
| title_full_unstemmed | Genetic fingerprinting of Theileria parva using a telomeric DNA probe |
| title_short | Genetic fingerprinting of Theileria parva using a telomeric DNA probe |
| title_sort | genetic fingerprinting of theileria parva using a telomeric dna probe |
| topic | dna fingerprinting theileria parva genetics |
| url | https://hdl.handle.net/10568/29473 |
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