Detection of Theileria parva in the salivary glands of Rhipicephalus appendiculatus: Evaluation of staining methods
A comparison of ten methods for staining tick salivary glands for detection of Theileria parva infection from ticks fed on rabbits for various periods was under taken. Staining with azure without hydrochloric acid hydrolysis was found to be the most reliable method for detection of the presporozoite...
| Main Authors: | , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
1995
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/29471 |
| _version_ | 1855515421578362880 |
|---|---|
| author | Voigt, Wolf P. Mwaura, S.N. Njihia, G.M. Nyaga, S.G. Young, A.S. |
| author_browse | Mwaura, S.N. Njihia, G.M. Nyaga, S.G. Voigt, Wolf P. Young, A.S. |
| author_facet | Voigt, Wolf P. Mwaura, S.N. Njihia, G.M. Nyaga, S.G. Young, A.S. |
| author_sort | Voigt, Wolf P. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | A comparison of ten methods for staining tick salivary glands for detection of Theileria parva infection from ticks fed on rabbits for various periods was under taken. Staining with azure without hydrochloric acid hydrolysis was found to be the most reliable method for detection of the presporozoite stage (sporoblasts) of T.parva in the salivary gland acini of unfed Rhipicephalus appendiculatus and could be applied to field ticks. All the stains proved suitable for the detection and quantitation of sporozoites in ticks fed for 4 days on rabbits. The capacity of the stains to allow detection of early stages of T. parva differed, but it became more reliable during tick feeding as sporoblasts developed and matured. Giemsa's stain and Feulgen's stain followed by superimposition of Giemsa's stain were superior to other stains for the detection and quantitation of immature salivary gland stages in feeding ticks. |
| format | Journal Article |
| id | CGSpace29471 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1995 |
| publishDateRange | 1995 |
| publishDateSort | 1995 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace294712024-04-25T06:00:21Z Detection of Theileria parva in the salivary glands of Rhipicephalus appendiculatus: Evaluation of staining methods Voigt, Wolf P. Mwaura, S.N. Njihia, G.M. Nyaga, S.G. Young, A.S. theileria parva salivary glands rhipicephalus appendiculatus animal diseases metastigmata infectious diseases parasitology A comparison of ten methods for staining tick salivary glands for detection of Theileria parva infection from ticks fed on rabbits for various periods was under taken. Staining with azure without hydrochloric acid hydrolysis was found to be the most reliable method for detection of the presporozoite stage (sporoblasts) of T.parva in the salivary gland acini of unfed Rhipicephalus appendiculatus and could be applied to field ticks. All the stains proved suitable for the detection and quantitation of sporozoites in ticks fed for 4 days on rabbits. The capacity of the stains to allow detection of early stages of T. parva differed, but it became more reliable during tick feeding as sporoblasts developed and matured. Giemsa's stain and Feulgen's stain followed by superimposition of Giemsa's stain were superior to other stains for the detection and quantitation of immature salivary gland stages in feeding ticks. 1995-01 2013-06-11T09:23:41Z 2013-06-11T09:23:41Z Journal Article https://hdl.handle.net/10568/29471 en Open Access Springer Parasitology Research;81(1): 74-81 |
| spellingShingle | theileria parva salivary glands rhipicephalus appendiculatus animal diseases metastigmata infectious diseases parasitology Voigt, Wolf P. Mwaura, S.N. Njihia, G.M. Nyaga, S.G. Young, A.S. Detection of Theileria parva in the salivary glands of Rhipicephalus appendiculatus: Evaluation of staining methods |
| title | Detection of Theileria parva in the salivary glands of Rhipicephalus appendiculatus: Evaluation of staining methods |
| title_full | Detection of Theileria parva in the salivary glands of Rhipicephalus appendiculatus: Evaluation of staining methods |
| title_fullStr | Detection of Theileria parva in the salivary glands of Rhipicephalus appendiculatus: Evaluation of staining methods |
| title_full_unstemmed | Detection of Theileria parva in the salivary glands of Rhipicephalus appendiculatus: Evaluation of staining methods |
| title_short | Detection of Theileria parva in the salivary glands of Rhipicephalus appendiculatus: Evaluation of staining methods |
| title_sort | detection of theileria parva in the salivary glands of rhipicephalus appendiculatus evaluation of staining methods |
| topic | theileria parva salivary glands rhipicephalus appendiculatus animal diseases metastigmata infectious diseases parasitology |
| url | https://hdl.handle.net/10568/29471 |
| work_keys_str_mv | AT voigtwolfp detectionoftheileriaparvainthesalivaryglandsofrhipicephalusappendiculatusevaluationofstainingmethods AT mwaurasn detectionoftheileriaparvainthesalivaryglandsofrhipicephalusappendiculatusevaluationofstainingmethods AT njihiagm detectionoftheileriaparvainthesalivaryglandsofrhipicephalusappendiculatusevaluationofstainingmethods AT nyagasg detectionoftheileriaparvainthesalivaryglandsofrhipicephalusappendiculatusevaluationofstainingmethods AT youngas detectionoftheileriaparvainthesalivaryglandsofrhipicephalusappendiculatusevaluationofstainingmethods |