Identification of the fragment containing cross-reacting antigenic determinants of the variable surface glycoprotein of Trypanosoma brucei
125I-labelled, isolated variable surface glycoproteins (VSGs) ofTrypanosoma b. bruceibind both homologous and heterologous anti-VSG sera and binding to heterologous antisera may be blocked by other unlabelled VSGs (Barbet & McGuire, 1978). This paper presents results which suggest that oligosacchari...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Cambridge University Press
1981
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/29457 |
| _version_ | 1855526941501685760 |
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| author | Barbet, A.F. Musoke, A.J. Shapiro, S.Z. Mpimbaza, G.W.N. McGuire, T.C. |
| author_browse | Barbet, A.F. McGuire, T.C. Mpimbaza, G.W.N. Musoke, A.J. Shapiro, S.Z. |
| author_facet | Barbet, A.F. Musoke, A.J. Shapiro, S.Z. Mpimbaza, G.W.N. McGuire, T.C. |
| author_sort | Barbet, A.F. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | 125I-labelled, isolated variable surface glycoproteins (VSGs) ofTrypanosoma b. bruceibind both homologous and heterologous anti-VSG sera and binding to heterologous antisera may be blocked by other unlabelled VSGs (Barbet & McGuire, 1978). This paper presents results which suggest that oligosaccharide residues have importance in the antigenic structure of VSG cross-reacting determinants. The ability of VSG to bind heterologous anti-VSG sera was destroyed by periodate oxidation but not by extensive proteolysis. A VSG glycopeptide fragment was isolated from two different VSGs, which blocked by 100 % the binding of VSG to heterologous anti-VSG sera and therefore contained the cross-reacting determinants. The native glycopeptide fragment was resistant to digestion with trypsin, pronase or leucine aminopeptidase and prolidase. We also show that a VSG synthesized in the reticulocyte lysate cell-free system was not immunoprecipitated by heterologous anti-VSG sera in contrast to the same VSG labelled by metabolic incorporation of [35S]methioninein vivo. |
| format | Journal Article |
| id | CGSpace29457 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1981 |
| publishDateRange | 1981 |
| publishDateSort | 1981 |
| publisher | Cambridge University Press |
| publisherStr | Cambridge University Press |
| record_format | dspace |
| spelling | CGSpace294572024-11-15T08:52:34Z Identification of the fragment containing cross-reacting antigenic determinants of the variable surface glycoprotein of Trypanosoma brucei Barbet, A.F. Musoke, A.J. Shapiro, S.Z. Mpimbaza, G.W.N. McGuire, T.C. antigens variants glycoproteins trypanosoma brucei animal diseases 125I-labelled, isolated variable surface glycoproteins (VSGs) ofTrypanosoma b. bruceibind both homologous and heterologous anti-VSG sera and binding to heterologous antisera may be blocked by other unlabelled VSGs (Barbet & McGuire, 1978). This paper presents results which suggest that oligosaccharide residues have importance in the antigenic structure of VSG cross-reacting determinants. The ability of VSG to bind heterologous anti-VSG sera was destroyed by periodate oxidation but not by extensive proteolysis. A VSG glycopeptide fragment was isolated from two different VSGs, which blocked by 100 % the binding of VSG to heterologous anti-VSG sera and therefore contained the cross-reacting determinants. The native glycopeptide fragment was resistant to digestion with trypsin, pronase or leucine aminopeptidase and prolidase. We also show that a VSG synthesized in the reticulocyte lysate cell-free system was not immunoprecipitated by heterologous anti-VSG sera in contrast to the same VSG labelled by metabolic incorporation of [35S]methioninein vivo. 1981-12 2013-06-11T09:23:38Z 2013-06-11T09:23:38Z Journal Article https://hdl.handle.net/10568/29457 en Limited Access Cambridge University Press Parasitology;83: 623-637 |
| spellingShingle | antigens variants glycoproteins trypanosoma brucei animal diseases Barbet, A.F. Musoke, A.J. Shapiro, S.Z. Mpimbaza, G.W.N. McGuire, T.C. Identification of the fragment containing cross-reacting antigenic determinants of the variable surface glycoprotein of Trypanosoma brucei |
| title | Identification of the fragment containing cross-reacting antigenic determinants of the variable surface glycoprotein of Trypanosoma brucei |
| title_full | Identification of the fragment containing cross-reacting antigenic determinants of the variable surface glycoprotein of Trypanosoma brucei |
| title_fullStr | Identification of the fragment containing cross-reacting antigenic determinants of the variable surface glycoprotein of Trypanosoma brucei |
| title_full_unstemmed | Identification of the fragment containing cross-reacting antigenic determinants of the variable surface glycoprotein of Trypanosoma brucei |
| title_short | Identification of the fragment containing cross-reacting antigenic determinants of the variable surface glycoprotein of Trypanosoma brucei |
| title_sort | identification of the fragment containing cross reacting antigenic determinants of the variable surface glycoprotein of trypanosoma brucei |
| topic | antigens variants glycoproteins trypanosoma brucei animal diseases |
| url | https://hdl.handle.net/10568/29457 |
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