Optimization of the in vitro feeding of Rhipicephalus appendiculatus nymphae for the transmission of Theileria parva
An apparatus for artificial feeding of Rhipicephalus appendiculatus nymphae was modified to improve feeding performance. Heparinized blood was supplied above a treated artificial membrane while the ticks attached below on its undersurface. The feeding apparatus was incubated at 37 °C in an atmospher...
| Autores principales: | , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Cambridge University Press
1995
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/29453 |
| _version_ | 1855529042863718400 |
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| author | Waladde, S.M. Young, A.S. Mwaura, S.N. Njihia, G.N. Mwakima, F.N. |
| author_browse | Mwakima, F.N. Mwaura, S.N. Njihia, G.N. Waladde, S.M. Young, A.S. |
| author_facet | Waladde, S.M. Young, A.S. Mwaura, S.N. Njihia, G.N. Mwakima, F.N. |
| author_sort | Waladde, S.M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | An apparatus for artificial feeding of Rhipicephalus appendiculatus nymphae was modified to improve feeding performance. Heparinized blood was supplied above a treated artificial membrane while the ticks attached below on its undersurface. The feeding apparatus was incubated at 37 °C in an atmosphere of 3% CO2 concentration and a relative humidity of 75–80%. Under these conditions, 91% of the engorged nymphae attained a mean weight of 6–11 mg, and an average of 93% of those nymphae moulted into adults. When this system was used to feed nymphal ticks on blood infected with Theileria parva piroplasms, the mean prevalence of infection in the resultant female and male ticks was 86% and 54%, respectively. The feeding performance and T. parva infection levels were comparable to those of nymphal ticks fed on the blood donor cattle. The apparatus used in this study has potential for modification to suit the artificial feeding needs of other species of ixodid ticks and for use in investigations to examine other tick/pathogen relationships. |
| format | Journal Article |
| id | CGSpace29453 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1995 |
| publishDateRange | 1995 |
| publishDateSort | 1995 |
| publisher | Cambridge University Press |
| publisherStr | Cambridge University Press |
| record_format | dspace |
| spelling | CGSpace294532024-11-15T08:52:40Z Optimization of the in vitro feeding of Rhipicephalus appendiculatus nymphae for the transmission of Theileria parva Waladde, S.M. Young, A.S. Mwaura, S.N. Njihia, G.N. Mwakima, F.N. rhipicephalus appendiculatus metastigmata theileria parva disease transmission An apparatus for artificial feeding of Rhipicephalus appendiculatus nymphae was modified to improve feeding performance. Heparinized blood was supplied above a treated artificial membrane while the ticks attached below on its undersurface. The feeding apparatus was incubated at 37 °C in an atmosphere of 3% CO2 concentration and a relative humidity of 75–80%. Under these conditions, 91% of the engorged nymphae attained a mean weight of 6–11 mg, and an average of 93% of those nymphae moulted into adults. When this system was used to feed nymphal ticks on blood infected with Theileria parva piroplasms, the mean prevalence of infection in the resultant female and male ticks was 86% and 54%, respectively. The feeding performance and T. parva infection levels were comparable to those of nymphal ticks fed on the blood donor cattle. The apparatus used in this study has potential for modification to suit the artificial feeding needs of other species of ixodid ticks and for use in investigations to examine other tick/pathogen relationships. 1995-11 2013-06-11T09:23:37Z 2013-06-11T09:23:37Z Journal Article https://hdl.handle.net/10568/29453 en Limited Access Cambridge University Press Parasitology;111(4): 463-468 |
| spellingShingle | rhipicephalus appendiculatus metastigmata theileria parva disease transmission Waladde, S.M. Young, A.S. Mwaura, S.N. Njihia, G.N. Mwakima, F.N. Optimization of the in vitro feeding of Rhipicephalus appendiculatus nymphae for the transmission of Theileria parva |
| title | Optimization of the in vitro feeding of Rhipicephalus appendiculatus nymphae for the transmission of Theileria parva |
| title_full | Optimization of the in vitro feeding of Rhipicephalus appendiculatus nymphae for the transmission of Theileria parva |
| title_fullStr | Optimization of the in vitro feeding of Rhipicephalus appendiculatus nymphae for the transmission of Theileria parva |
| title_full_unstemmed | Optimization of the in vitro feeding of Rhipicephalus appendiculatus nymphae for the transmission of Theileria parva |
| title_short | Optimization of the in vitro feeding of Rhipicephalus appendiculatus nymphae for the transmission of Theileria parva |
| title_sort | optimization of the in vitro feeding of rhipicephalus appendiculatus nymphae for the transmission of theileria parva |
| topic | rhipicephalus appendiculatus metastigmata theileria parva disease transmission |
| url | https://hdl.handle.net/10568/29453 |
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