Discrimination between six species of Theileria using oligonucleotide probes which detect small subunit ribosomal RNA sequences
The complete small subunit ribosomal RNA (srRNA) gene of Theileria parva was cloned and sequenced. Two primers were designed which permitted the specific amplification of part of the Theileria srRNA gene from Theileria-infected cell line samples which were predominantly (> 95%) bovine DNA. The seque...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Cambridge University Press
1993
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/29443 |
| _version_ | 1855542739474579456 |
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| author | Allsopp, B.A. Bayles, H.A. Allsopp, M.T. Cavalier-Smith, T. Bishop, Richard P. Carrington, D.M. Sohanpal, B.K. Spooner, P.R. |
| author_browse | Allsopp, B.A. Allsopp, M.T. Bayles, H.A. Bishop, Richard P. Carrington, D.M. Cavalier-Smith, T. Sohanpal, B.K. Spooner, P.R. |
| author_facet | Allsopp, B.A. Bayles, H.A. Allsopp, M.T. Cavalier-Smith, T. Bishop, Richard P. Carrington, D.M. Sohanpal, B.K. Spooner, P.R. |
| author_sort | Allsopp, B.A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The complete small subunit ribosomal RNA (srRNA) gene of Theileria parva was cloned and sequenced. Two primers were designed which permitted the specific amplification of part of the Theileria srRNA gene from Theileria-infected cell line samples which were predominantly (> 95%) bovine DNA. The sequence of the central (variable) region of the srRNA genes of T. annulata, T. taurotragi, T. mutans and two unidentified parasites referred to as Theileria sp. (buffalo) and Theileria sp. (Marula) were obtained. An alignment of the sequences was generated from which 6 oligonucleotide probes, corresponding to species-specific regions, were designed. These probes were demonstrated to provide unequivocal identification of each of the 6 species either by direct detection of parasite srRNA or by hybridization to amplified parasite srRNA genes. The probes were not able to distinguish buffalo-derived T. parva, the causal agent of Corridor disease, from cattle-derived T. parva, the causal agent of East Coast fever. |
| format | Journal Article |
| id | CGSpace29443 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1993 |
| publishDateRange | 1993 |
| publishDateSort | 1993 |
| publisher | Cambridge University Press |
| publisherStr | Cambridge University Press |
| record_format | dspace |
| spelling | CGSpace294432024-11-15T08:53:12Z Discrimination between six species of Theileria using oligonucleotide probes which detect small subunit ribosomal RNA sequences Allsopp, B.A. Bayles, H.A. Allsopp, M.T. Cavalier-Smith, T. Bishop, Richard P. Carrington, D.M. Sohanpal, B.K. Spooner, P.R. theileria metastigmata nucleotides dna animal diseases The complete small subunit ribosomal RNA (srRNA) gene of Theileria parva was cloned and sequenced. Two primers were designed which permitted the specific amplification of part of the Theileria srRNA gene from Theileria-infected cell line samples which were predominantly (> 95%) bovine DNA. The sequence of the central (variable) region of the srRNA genes of T. annulata, T. taurotragi, T. mutans and two unidentified parasites referred to as Theileria sp. (buffalo) and Theileria sp. (Marula) were obtained. An alignment of the sequences was generated from which 6 oligonucleotide probes, corresponding to species-specific regions, were designed. These probes were demonstrated to provide unequivocal identification of each of the 6 species either by direct detection of parasite srRNA or by hybridization to amplified parasite srRNA genes. The probes were not able to distinguish buffalo-derived T. parva, the causal agent of Corridor disease, from cattle-derived T. parva, the causal agent of East Coast fever. 1993-08 2013-06-11T09:23:35Z 2013-06-11T09:23:35Z Journal Article https://hdl.handle.net/10568/29443 en Limited Access Cambridge University Press Parasitology;107: 157-165 |
| spellingShingle | theileria metastigmata nucleotides dna animal diseases Allsopp, B.A. Bayles, H.A. Allsopp, M.T. Cavalier-Smith, T. Bishop, Richard P. Carrington, D.M. Sohanpal, B.K. Spooner, P.R. Discrimination between six species of Theileria using oligonucleotide probes which detect small subunit ribosomal RNA sequences |
| title | Discrimination between six species of Theileria using oligonucleotide probes which detect small subunit ribosomal RNA sequences |
| title_full | Discrimination between six species of Theileria using oligonucleotide probes which detect small subunit ribosomal RNA sequences |
| title_fullStr | Discrimination between six species of Theileria using oligonucleotide probes which detect small subunit ribosomal RNA sequences |
| title_full_unstemmed | Discrimination between six species of Theileria using oligonucleotide probes which detect small subunit ribosomal RNA sequences |
| title_short | Discrimination between six species of Theileria using oligonucleotide probes which detect small subunit ribosomal RNA sequences |
| title_sort | discrimination between six species of theileria using oligonucleotide probes which detect small subunit ribosomal rna sequences |
| topic | theileria metastigmata nucleotides dna animal diseases |
| url | https://hdl.handle.net/10568/29443 |
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