Detection of polymorphisms among Theileria parva stocks using repetitive, telomeric ribosomal DNA probes and anti-schizont monoclonal antibodies
A total of 21Theileria parvastocks from 6 countries were characterized usingT. parvarepetitive and ribosomal DNA probes, aPlasmodium bergheitelomeric oligonucleotide and a panel of anti-schizont monoclonal antibodies (MAbs). Hybridization of the repetitive DNA probe to Southern blots ofEcoRI-digeste...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Cambridge University Press
1993
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/29440 |
| _version_ | 1855535186748375040 |
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| author | Bishop, Richard P. Sohanpal, B.K. Spooner, P.R. Dolan, T.T. Allsopp, B.A. Morzaria, S.P. |
| author_browse | Allsopp, B.A. Bishop, Richard P. Dolan, T.T. Morzaria, S.P. Sohanpal, B.K. Spooner, P.R. |
| author_facet | Bishop, Richard P. Sohanpal, B.K. Spooner, P.R. Dolan, T.T. Allsopp, B.A. Morzaria, S.P. |
| author_sort | Bishop, Richard P. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | A total of 21Theileria parvastocks from 6 countries were characterized usingT. parvarepetitive and ribosomal DNA probes, aPlasmodium bergheitelomeric oligonucleotide and a panel of anti-schizont monoclonal antibodies (MAbs). Hybridization of the repetitive DNA probe to Southern blots ofEcoRI-digestedT. parvaDNA revealed 20 different restriction fragment patterns among DNA samples isolated from infections initiated using 16 parasite stocks. The panel of anti-schizont MAbs defined 8 different profiles among schizont-infected lymphoblastoid cell-cultures infected with the same 16T. parvastocks. Many stocks, which were differentiated by the repetitive DNA probe, could not be distinguished using the anti-schizont MAbs. A clonedT. parvasmall subunit ribosomal RNA (SSUrRNA) gene probe separated 17T. parvastocks into 2 groups, exhibiting either 1 or 2 restriction fragments, when hybridized toEcoRI-digestedT. parvaDNA. When hybridized toPvuII-digested DNA from 8T. parvastocks, the ribosomal probe identified 4 groups with similar restriction fragment patterns. A synthetic oligonucleotide derived from aP. bergheitelomeric sequence hybridized to 7 or 8 size-polymorphic restriction fragments in theEcoRI-digested DNA of mostT. parvastocks. The telomeric and ribosomal probes defined the same 4 groups among 8T. parvastocks as assessed by similarities in restriction fragment patterns. Based on the comparison of repetitive DNA sequences from theT. parvaUganda and Muguga stocks, a synthetic oligonucleotide was developed which distinguished the DNA of theT. parvaUganda stock from that of 4 otherT. parvastocks on a positive/negative basis. |
| format | Journal Article |
| id | CGSpace29440 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1993 |
| publishDateRange | 1993 |
| publishDateSort | 1993 |
| publisher | Cambridge University Press |
| publisherStr | Cambridge University Press |
| record_format | dspace |
| spelling | CGSpace294402024-11-15T08:52:55Z Detection of polymorphisms among Theileria parva stocks using repetitive, telomeric ribosomal DNA probes and anti-schizont monoclonal antibodies Bishop, Richard P. Sohanpal, B.K. Spooner, P.R. Dolan, T.T. Allsopp, B.A. Morzaria, S.P. theileria parva monoclonal antibodies dna animal diseases A total of 21Theileria parvastocks from 6 countries were characterized usingT. parvarepetitive and ribosomal DNA probes, aPlasmodium bergheitelomeric oligonucleotide and a panel of anti-schizont monoclonal antibodies (MAbs). Hybridization of the repetitive DNA probe to Southern blots ofEcoRI-digestedT. parvaDNA revealed 20 different restriction fragment patterns among DNA samples isolated from infections initiated using 16 parasite stocks. The panel of anti-schizont MAbs defined 8 different profiles among schizont-infected lymphoblastoid cell-cultures infected with the same 16T. parvastocks. Many stocks, which were differentiated by the repetitive DNA probe, could not be distinguished using the anti-schizont MAbs. A clonedT. parvasmall subunit ribosomal RNA (SSUrRNA) gene probe separated 17T. parvastocks into 2 groups, exhibiting either 1 or 2 restriction fragments, when hybridized toEcoRI-digestedT. parvaDNA. When hybridized toPvuII-digested DNA from 8T. parvastocks, the ribosomal probe identified 4 groups with similar restriction fragment patterns. A synthetic oligonucleotide derived from aP. bergheitelomeric sequence hybridized to 7 or 8 size-polymorphic restriction fragments in theEcoRI-digested DNA of mostT. parvastocks. The telomeric and ribosomal probes defined the same 4 groups among 8T. parvastocks as assessed by similarities in restriction fragment patterns. Based on the comparison of repetitive DNA sequences from theT. parvaUganda and Muguga stocks, a synthetic oligonucleotide was developed which distinguished the DNA of theT. parvaUganda stock from that of 4 otherT. parvastocks on a positive/negative basis. 1993-07 2013-06-11T09:23:34Z 2013-06-11T09:23:34Z Journal Article https://hdl.handle.net/10568/29440 en Limited Access Cambridge University Press Parasitology;107: 19-31 |
| spellingShingle | theileria parva monoclonal antibodies dna animal diseases Bishop, Richard P. Sohanpal, B.K. Spooner, P.R. Dolan, T.T. Allsopp, B.A. Morzaria, S.P. Detection of polymorphisms among Theileria parva stocks using repetitive, telomeric ribosomal DNA probes and anti-schizont monoclonal antibodies |
| title | Detection of polymorphisms among Theileria parva stocks using repetitive, telomeric ribosomal DNA probes and anti-schizont monoclonal antibodies |
| title_full | Detection of polymorphisms among Theileria parva stocks using repetitive, telomeric ribosomal DNA probes and anti-schizont monoclonal antibodies |
| title_fullStr | Detection of polymorphisms among Theileria parva stocks using repetitive, telomeric ribosomal DNA probes and anti-schizont monoclonal antibodies |
| title_full_unstemmed | Detection of polymorphisms among Theileria parva stocks using repetitive, telomeric ribosomal DNA probes and anti-schizont monoclonal antibodies |
| title_short | Detection of polymorphisms among Theileria parva stocks using repetitive, telomeric ribosomal DNA probes and anti-schizont monoclonal antibodies |
| title_sort | detection of polymorphisms among theileria parva stocks using repetitive telomeric ribosomal dna probes and anti schizont monoclonal antibodies |
| topic | theileria parva monoclonal antibodies dna animal diseases |
| url | https://hdl.handle.net/10568/29440 |
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