Characterization of buffalo-derived theilerial parasites with monoclonal antibodies and DNA probes
The characteristics of intra-lymphocyticTheileriaisolated from African buffalo and from cattle that were infected with buffalo-derived parasites were evaluated using anti-schizont monoclonal antibodies (mAbs) and DNA probes. Antigenic differences were revealed by the reactivities of 27 mAbs with the...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Cambridge University Press
1989
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/29434 |
| _version_ | 1855520406384934912 |
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| author | Conrad, P.A. Ole-MoiYoi, O.K. Baldwin, Cynthia L. Dolan, T.T. O'Callaghan, C.J. Njamunggeh, R.E.G. Grootenhuis, J.G. Stagg, D.A. Leitch, B.L. Young, A.S. |
| author_browse | Baldwin, Cynthia L. Conrad, P.A. Dolan, T.T. Grootenhuis, J.G. Leitch, B.L. Njamunggeh, R.E.G. O'Callaghan, C.J. Ole-MoiYoi, O.K. Stagg, D.A. Young, A.S. |
| author_facet | Conrad, P.A. Ole-MoiYoi, O.K. Baldwin, Cynthia L. Dolan, T.T. O'Callaghan, C.J. Njamunggeh, R.E.G. Grootenhuis, J.G. Stagg, D.A. Leitch, B.L. Young, A.S. |
| author_sort | Conrad, P.A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The characteristics of intra-lymphocyticTheileriaisolated from African buffalo and from cattle that were infected with buffalo-derived parasites were evaluated using anti-schizont monoclonal antibodies (mAbs) and DNA probes. Antigenic differences were revealed by the reactivities of 27 mAbs with the buffalo-derived parasites isolated from different animals. Antigenic diversity was also seen withTheileria-infected lymphoblastoid cell isolates taken from the lymph nodes and blood of the same animals. Two DNA probes, selected from a genomic library ofT. parvapiroplasm DNA cloned inλgt11, showed specific hybridization to parasite DNA in Southern blots of restriction enzyme-digested, lymphoblastoid cells infected with buffalo-derived theilerial parasites. Genotypic differences between the buffalo-derived parasites were revealed by the restriction fragment length polymorphisms seen with hybridization of those probes to DNA from cloned and unclonedTheileria-infected cell lines. The evaluation of theilerial parasites derived from buffalo and from cattle which underwent typicalT. p. lawrenceireactions, after being infected with buffalo-derived theilerial parasites, did not show any specific phenotypic or genotypic characteristics of these parasites that would distinguish them fromT. p. parvaandT. p. bovisparasites. The validity of these subspecies distinctions is discussed. |
| format | Journal Article |
| id | CGSpace29434 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1989 |
| publishDateRange | 1989 |
| publishDateSort | 1989 |
| publisher | Cambridge University Press |
| publisherStr | Cambridge University Press |
| record_format | dspace |
| spelling | CGSpace294342024-11-15T08:52:20Z Characterization of buffalo-derived theilerial parasites with monoclonal antibodies and DNA probes Conrad, P.A. Ole-MoiYoi, O.K. Baldwin, Cynthia L. Dolan, T.T. O'Callaghan, C.J. Njamunggeh, R.E.G. Grootenhuis, J.G. Stagg, D.A. Leitch, B.L. Young, A.S. dna parasites theileria buffaloes monoclonal antibodies disease control immunity The characteristics of intra-lymphocyticTheileriaisolated from African buffalo and from cattle that were infected with buffalo-derived parasites were evaluated using anti-schizont monoclonal antibodies (mAbs) and DNA probes. Antigenic differences were revealed by the reactivities of 27 mAbs with the buffalo-derived parasites isolated from different animals. Antigenic diversity was also seen withTheileria-infected lymphoblastoid cell isolates taken from the lymph nodes and blood of the same animals. Two DNA probes, selected from a genomic library ofT. parvapiroplasm DNA cloned inλgt11, showed specific hybridization to parasite DNA in Southern blots of restriction enzyme-digested, lymphoblastoid cells infected with buffalo-derived theilerial parasites. Genotypic differences between the buffalo-derived parasites were revealed by the restriction fragment length polymorphisms seen with hybridization of those probes to DNA from cloned and unclonedTheileria-infected cell lines. The evaluation of theilerial parasites derived from buffalo and from cattle which underwent typicalT. p. lawrenceireactions, after being infected with buffalo-derived theilerial parasites, did not show any specific phenotypic or genotypic characteristics of these parasites that would distinguish them fromT. p. parvaandT. p. bovisparasites. The validity of these subspecies distinctions is discussed. 1989-04 2013-06-11T09:23:33Z 2013-06-11T09:23:33Z Journal Article https://hdl.handle.net/10568/29434 en Limited Access Cambridge University Press Parasitology;98: 179-188 |
| spellingShingle | dna parasites theileria buffaloes monoclonal antibodies disease control immunity Conrad, P.A. Ole-MoiYoi, O.K. Baldwin, Cynthia L. Dolan, T.T. O'Callaghan, C.J. Njamunggeh, R.E.G. Grootenhuis, J.G. Stagg, D.A. Leitch, B.L. Young, A.S. Characterization of buffalo-derived theilerial parasites with monoclonal antibodies and DNA probes |
| title | Characterization of buffalo-derived theilerial parasites with monoclonal antibodies and DNA probes |
| title_full | Characterization of buffalo-derived theilerial parasites with monoclonal antibodies and DNA probes |
| title_fullStr | Characterization of buffalo-derived theilerial parasites with monoclonal antibodies and DNA probes |
| title_full_unstemmed | Characterization of buffalo-derived theilerial parasites with monoclonal antibodies and DNA probes |
| title_short | Characterization of buffalo-derived theilerial parasites with monoclonal antibodies and DNA probes |
| title_sort | characterization of buffalo derived theilerial parasites with monoclonal antibodies and dna probes |
| topic | dna parasites theileria buffaloes monoclonal antibodies disease control immunity |
| url | https://hdl.handle.net/10568/29434 |
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