Use of species-specific DNA probes for detection and identification of trypanosome infection in tsetse flies
Species- and subspecies-specific trypanosome DNA hybridization probes have been employed in the detection and identification of trypanosome infections inGlossina morsitans centralis.Several ways of sample preparation including the use of tsetse organ suspensions, proboscides and dissected midguts, a...
| Autores principales: | , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Cambridge University Press
1987
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/29427 |
| _version_ | 1855541285033607168 |
|---|---|
| author | Kukla, B.A. Majiwa, Phelix A.O. Young, J.R. Moloo, S.K. Ole-MoiYoi, O.K. |
| author_browse | Kukla, B.A. Majiwa, Phelix A.O. Moloo, S.K. Ole-MoiYoi, O.K. Young, J.R. |
| author_facet | Kukla, B.A. Majiwa, Phelix A.O. Young, J.R. Moloo, S.K. Ole-MoiYoi, O.K. |
| author_sort | Kukla, B.A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Species- and subspecies-specific trypanosome DNA hybridization probes have been employed in the detection and identification of trypanosome infections inGlossina morsitans centralis.Several ways of sample preparation including the use of tsetse organ suspensions, proboscides and dissected midguts, as well as tsetse abdominal content touch-blots were explored. The results of hybridization of radio-isotope-labelled species-specific DNA probes to tsetse samples indicated that it was possible to detect trypanosomes in the organs where parasite development is known to characteristically occur for each subgenus. Duplicate slot-blots of samples prepared from midguts of tsetse infected with 2 strains ofT. congolenseand from non-infected fly controls show that it is not only possible to detect infection in tsetse but also to identify the strain of parasite present in a sample after hybridization with the DNA probes specific for each strain. The results, obtained after hybridization of sequential abdominal touch-blots from the same fly with the DNA probe specific for one strain ofT. congolense,indicated that at least 8 positive signals can be observed after an overnight exposure. Because of their simplicity and potentially low cost, the techniques described here would be appealing for screening large numbers of tsetse samples from the field for the presence of any trypanosome residing in the guts or proboscis of the vector. In addition, the possibility of doing multiple touch-blots from the same fly gives the opportunity of detecting mixed trypanosome infections in the vector. |
| format | Journal Article |
| id | CGSpace29427 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1987 |
| publishDateRange | 1987 |
| publishDateSort | 1987 |
| publisher | Cambridge University Press |
| publisherStr | Cambridge University Press |
| record_format | dspace |
| spelling | CGSpace294272024-11-15T08:53:09Z Use of species-specific DNA probes for detection and identification of trypanosome infection in tsetse flies Kukla, B.A. Majiwa, Phelix A.O. Young, J.R. Moloo, S.K. Ole-MoiYoi, O.K. trypanosoma diagnosis glossinidae dna animal diseases Species- and subspecies-specific trypanosome DNA hybridization probes have been employed in the detection and identification of trypanosome infections inGlossina morsitans centralis.Several ways of sample preparation including the use of tsetse organ suspensions, proboscides and dissected midguts, as well as tsetse abdominal content touch-blots were explored. The results of hybridization of radio-isotope-labelled species-specific DNA probes to tsetse samples indicated that it was possible to detect trypanosomes in the organs where parasite development is known to characteristically occur for each subgenus. Duplicate slot-blots of samples prepared from midguts of tsetse infected with 2 strains ofT. congolenseand from non-infected fly controls show that it is not only possible to detect infection in tsetse but also to identify the strain of parasite present in a sample after hybridization with the DNA probes specific for each strain. The results, obtained after hybridization of sequential abdominal touch-blots from the same fly with the DNA probe specific for one strain ofT. congolense,indicated that at least 8 positive signals can be observed after an overnight exposure. Because of their simplicity and potentially low cost, the techniques described here would be appealing for screening large numbers of tsetse samples from the field for the presence of any trypanosome residing in the guts or proboscis of the vector. In addition, the possibility of doing multiple touch-blots from the same fly gives the opportunity of detecting mixed trypanosome infections in the vector. 1987-08 2013-06-11T09:23:31Z 2013-06-11T09:23:31Z Journal Article https://hdl.handle.net/10568/29427 en Limited Access Cambridge University Press Parasitology;95: 1-16 |
| spellingShingle | trypanosoma diagnosis glossinidae dna animal diseases Kukla, B.A. Majiwa, Phelix A.O. Young, J.R. Moloo, S.K. Ole-MoiYoi, O.K. Use of species-specific DNA probes for detection and identification of trypanosome infection in tsetse flies |
| title | Use of species-specific DNA probes for detection and identification of trypanosome infection in tsetse flies |
| title_full | Use of species-specific DNA probes for detection and identification of trypanosome infection in tsetse flies |
| title_fullStr | Use of species-specific DNA probes for detection and identification of trypanosome infection in tsetse flies |
| title_full_unstemmed | Use of species-specific DNA probes for detection and identification of trypanosome infection in tsetse flies |
| title_short | Use of species-specific DNA probes for detection and identification of trypanosome infection in tsetse flies |
| title_sort | use of species specific dna probes for detection and identification of trypanosome infection in tsetse flies |
| topic | trypanosoma diagnosis glossinidae dna animal diseases |
| url | https://hdl.handle.net/10568/29427 |
| work_keys_str_mv | AT kuklaba useofspeciesspecificdnaprobesfordetectionandidentificationoftrypanosomeinfectionintsetseflies AT majiwaphelixao useofspeciesspecificdnaprobesfordetectionandidentificationoftrypanosomeinfectionintsetseflies AT youngjr useofspeciesspecificdnaprobesfordetectionandidentificationoftrypanosomeinfectionintsetseflies AT moloosk useofspeciesspecificdnaprobesfordetectionandidentificationoftrypanosomeinfectionintsetseflies AT olemoiyoiok useofspeciesspecificdnaprobesfordetectionandidentificationoftrypanosomeinfectionintsetseflies |