Trypanosoma (Nannomonas) congolense. Molecular characterization of a new genotype from Tsavo, Kenya
Trypanosoma (Nannomonas) congolense comprises morphologically identical but genetically heterogeneous parasites infective to livestock and other mammalian hosts; three different genotypes of this parasite have been described previously. Restriction enzyme fragment length polymorphisms (RFLPs) in bot...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Cambridge University Press
1993
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/29426 |
| _version_ | 1855524632872878080 |
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| author | Majiwa, Phelix A.O. Maina, M. Waitumbi, J.N. Mihok, S. Zweygarth, E. |
| author_browse | Maina, M. Majiwa, Phelix A.O. Mihok, S. Waitumbi, J.N. Zweygarth, E. |
| author_facet | Majiwa, Phelix A.O. Maina, M. Waitumbi, J.N. Mihok, S. Zweygarth, E. |
| author_sort | Majiwa, Phelix A.O. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Trypanosoma (Nannomonas) congolense comprises morphologically identical but genetically heterogeneous parasites infective to livestock and other mammalian hosts; three different genotypes of this parasite have been described previously. Restriction enzyme fragment length polymorphisms (RFLPs) in both kinetoplast DNA minicircle and nuclear DNA sequences, and randomly amplified polymorphic deoxyribonucleic acid (RAPD) patterns have been used here to demonstrate the existence of another type of T. (N.) congolense that is genotypically distinct from those that have so far been characterized at the molecular level. A highly repetitive, tandemly arranged DNA sequence and oligonucleotide primers, for use in polymerase chain reaction (PCR) amplification are described, which can be used for specific identification of the trypanosome and its distinction from others within the Nannomonas subgenus. |
| format | Journal Article |
| id | CGSpace29426 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1993 |
| publishDateRange | 1993 |
| publishDateSort | 1993 |
| publisher | Cambridge University Press |
| publisherStr | Cambridge University Press |
| record_format | dspace |
| spelling | CGSpace294262024-11-15T08:52:28Z Trypanosoma (Nannomonas) congolense. Molecular characterization of a new genotype from Tsavo, Kenya Majiwa, Phelix A.O. Maina, M. Waitumbi, J.N. Mihok, S. Zweygarth, E. genes trypanosoma congolense animal diseases Trypanosoma (Nannomonas) congolense comprises morphologically identical but genetically heterogeneous parasites infective to livestock and other mammalian hosts; three different genotypes of this parasite have been described previously. Restriction enzyme fragment length polymorphisms (RFLPs) in both kinetoplast DNA minicircle and nuclear DNA sequences, and randomly amplified polymorphic deoxyribonucleic acid (RAPD) patterns have been used here to demonstrate the existence of another type of T. (N.) congolense that is genotypically distinct from those that have so far been characterized at the molecular level. A highly repetitive, tandemly arranged DNA sequence and oligonucleotide primers, for use in polymerase chain reaction (PCR) amplification are described, which can be used for specific identification of the trypanosome and its distinction from others within the Nannomonas subgenus. 1993-02 2013-06-11T09:23:31Z 2013-06-11T09:23:31Z Journal Article https://hdl.handle.net/10568/29426 en Limited Access Cambridge University Press Parasitology;106: 151-162 |
| spellingShingle | genes trypanosoma congolense animal diseases Majiwa, Phelix A.O. Maina, M. Waitumbi, J.N. Mihok, S. Zweygarth, E. Trypanosoma (Nannomonas) congolense. Molecular characterization of a new genotype from Tsavo, Kenya |
| title | Trypanosoma (Nannomonas) congolense. Molecular characterization of a new genotype from Tsavo, Kenya |
| title_full | Trypanosoma (Nannomonas) congolense. Molecular characterization of a new genotype from Tsavo, Kenya |
| title_fullStr | Trypanosoma (Nannomonas) congolense. Molecular characterization of a new genotype from Tsavo, Kenya |
| title_full_unstemmed | Trypanosoma (Nannomonas) congolense. Molecular characterization of a new genotype from Tsavo, Kenya |
| title_short | Trypanosoma (Nannomonas) congolense. Molecular characterization of a new genotype from Tsavo, Kenya |
| title_sort | trypanosoma nannomonas congolense molecular characterization of a new genotype from tsavo kenya |
| topic | genes trypanosoma congolense animal diseases |
| url | https://hdl.handle.net/10568/29426 |
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