Peptidase in the plasma of mice infected with Trypanosoma brucei brucei
The plasma of mice infected with pleomorphicTrypanosoma brucei bruceicontains a peptidase which has the same electrophoretic mobility on starch gels as a parasite peptidase. An enzyme with this electrophoretic mobility was not detected in the plasma of uninfected mice. The molecular weight of this e...
| Main Authors: | , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Cambridge University Press
1987
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/29423 |
| _version_ | 1855516649951592448 |
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| author | Knowles, G. Black, Samuel J. Whitelaw, D.D. |
| author_browse | Black, Samuel J. Knowles, G. Whitelaw, D.D. |
| author_facet | Knowles, G. Black, Samuel J. Whitelaw, D.D. |
| author_sort | Knowles, G. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The plasma of mice infected with pleomorphicTrypanosoma brucei bruceicontains a peptidase which has the same electrophoretic mobility on starch gels as a parasite peptidase. An enzyme with this electrophoretic mobility was not detected in the plasma of uninfected mice. The molecular weight of this enzyme in either parasite lysate or plasma from infected mice was approximately 40000 Da when assayed on a size exclusion column using high-performance liquid chromatography. The enzyme can cleave the dipeptides leu-ala, val-leu and pro-leu, but not the dipeptide phe-ala. The enzyme also cleaved the tripeptides tyr-tyr-tyr and leu-gly-gly. Another parasite peptidase which migrates on starch gels to a different position than the above-mentioned peptidase cleaved the dipeptides leu-ala, val-leu and pro-leu but could not cleave the tripeptides tyr-tyr-tyr or leu-gly-gly. Furthermore, incubation of this parasite peptidase with normal mouse plasma at 37 °C resulted in an apparent loss of detectable activity. It is postulated that the plasma of mice modifies either the charge or enzymic activity of this peptidase. We speculate that the parasite peptidase present in the plasma of mice infected withT. bruceicould contribute to pathogenesis. |
| format | Journal Article |
| id | CGSpace29423 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1987 |
| publishDateRange | 1987 |
| publishDateSort | 1987 |
| publisher | Cambridge University Press |
| publisherStr | Cambridge University Press |
| record_format | dspace |
| spelling | CGSpace294232024-11-15T08:52:11Z Peptidase in the plasma of mice infected with Trypanosoma brucei brucei Knowles, G. Black, Samuel J. Whitelaw, D.D. trypanosoma brucei peptidases mice plasma animal diseases infectious diseases parasitology The plasma of mice infected with pleomorphicTrypanosoma brucei bruceicontains a peptidase which has the same electrophoretic mobility on starch gels as a parasite peptidase. An enzyme with this electrophoretic mobility was not detected in the plasma of uninfected mice. The molecular weight of this enzyme in either parasite lysate or plasma from infected mice was approximately 40000 Da when assayed on a size exclusion column using high-performance liquid chromatography. The enzyme can cleave the dipeptides leu-ala, val-leu and pro-leu, but not the dipeptide phe-ala. The enzyme also cleaved the tripeptides tyr-tyr-tyr and leu-gly-gly. Another parasite peptidase which migrates on starch gels to a different position than the above-mentioned peptidase cleaved the dipeptides leu-ala, val-leu and pro-leu but could not cleave the tripeptides tyr-tyr-tyr or leu-gly-gly. Furthermore, incubation of this parasite peptidase with normal mouse plasma at 37 °C resulted in an apparent loss of detectable activity. It is postulated that the plasma of mice modifies either the charge or enzymic activity of this peptidase. We speculate that the parasite peptidase present in the plasma of mice infected withT. bruceicould contribute to pathogenesis. 1987-10 2013-06-11T09:23:31Z 2013-06-11T09:23:31Z Journal Article https://hdl.handle.net/10568/29423 en Limited Access Cambridge University Press Parasitology;95: 291-300 |
| spellingShingle | trypanosoma brucei peptidases mice plasma animal diseases infectious diseases parasitology Knowles, G. Black, Samuel J. Whitelaw, D.D. Peptidase in the plasma of mice infected with Trypanosoma brucei brucei |
| title | Peptidase in the plasma of mice infected with Trypanosoma brucei brucei |
| title_full | Peptidase in the plasma of mice infected with Trypanosoma brucei brucei |
| title_fullStr | Peptidase in the plasma of mice infected with Trypanosoma brucei brucei |
| title_full_unstemmed | Peptidase in the plasma of mice infected with Trypanosoma brucei brucei |
| title_short | Peptidase in the plasma of mice infected with Trypanosoma brucei brucei |
| title_sort | peptidase in the plasma of mice infected with trypanosoma brucei brucei |
| topic | trypanosoma brucei peptidases mice plasma animal diseases infectious diseases parasitology |
| url | https://hdl.handle.net/10568/29423 |
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