Parasite development and host responses during the establishment of Trypanosoma brucei infection transmitted by tsetse fly

Following inoculation ofTrypanosoma bruceiinto large mammals by the tsetse fly a local skin reaction, the ‘chancre’, develops due to trypanosome proliferation. We have cannulated the afferent and efferent lymphatics of the draining lymph node in goats and examined the onset of a cellular reaction, t...

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Autores principales: Barry, J.D., Emery, J.L.
Formato: Journal Article
Lenguaje:Inglés
Publicado: Cambridge University Press 1984
Materias:
Acceso en línea:https://hdl.handle.net/10568/29422
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author Barry, J.D.
Emery, J.L.
author_browse Barry, J.D.
Emery, J.L.
author_facet Barry, J.D.
Emery, J.L.
author_sort Barry, J.D.
collection Repository of Agricultural Research Outputs (CGSpace)
description Following inoculation ofTrypanosoma bruceiinto large mammals by the tsetse fly a local skin reaction, the ‘chancre’, develops due to trypanosome proliferation. We have cannulated the afferent and efferent lymphatics of the draining lymph node in goats and examined the onset of a cellular reaction, the emigration of the parasite from the chancre and the development of both antigenic variation and the specific immune response. The chancre first became detectable by day 3 post-infection, peaked by day 6 and then subsided. Lymphocyte output increased 6- to 8-fold by day 10 and the number of lymphoblasts increased 50-fold in this period. Both then declined. Trypanosomes were detected in lymph 1–2 days before the chancre, peaked by days 5–6, declined during development of the chancre and then peaked again. The bloodstream population appeared by days 4–5 and displayed different kinetics from that in lymph. Recirculation of parasites through the lymphatics ensued. Lymph-borne trypanosome populations were highly pleomorphic. Parasites in lymph expressed firstly a mixture of the Variable Antigen Types (VATs) which are found characteristically in the tsetse fly, this being followed by a mixture of other VATs. The two groups overlapped in appearance. In the bloodstream the same sequence of events occurred although 2 or 3 days later. The specific antibody response, as measured by radioimmunoassay and agglutination, arose within a few days of the first detection of each VAT. Activities appeared first in the lymph and then in plasma.
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spelling CGSpace294222024-11-15T08:52:14Z Parasite development and host responses during the establishment of Trypanosoma brucei infection transmitted by tsetse fly Barry, J.D. Emery, J.L. trypanosoma brucei parasites host parasite relations disease transmission infection glossinidae infectious diseases parasitology Following inoculation ofTrypanosoma bruceiinto large mammals by the tsetse fly a local skin reaction, the ‘chancre’, develops due to trypanosome proliferation. We have cannulated the afferent and efferent lymphatics of the draining lymph node in goats and examined the onset of a cellular reaction, the emigration of the parasite from the chancre and the development of both antigenic variation and the specific immune response. The chancre first became detectable by day 3 post-infection, peaked by day 6 and then subsided. Lymphocyte output increased 6- to 8-fold by day 10 and the number of lymphoblasts increased 50-fold in this period. Both then declined. Trypanosomes were detected in lymph 1–2 days before the chancre, peaked by days 5–6, declined during development of the chancre and then peaked again. The bloodstream population appeared by days 4–5 and displayed different kinetics from that in lymph. Recirculation of parasites through the lymphatics ensued. Lymph-borne trypanosome populations were highly pleomorphic. Parasites in lymph expressed firstly a mixture of the Variable Antigen Types (VATs) which are found characteristically in the tsetse fly, this being followed by a mixture of other VATs. The two groups overlapped in appearance. In the bloodstream the same sequence of events occurred although 2 or 3 days later. The specific antibody response, as measured by radioimmunoassay and agglutination, arose within a few days of the first detection of each VAT. Activities appeared first in the lymph and then in plasma. 1984-02 2013-06-11T09:23:30Z 2013-06-11T09:23:30Z Journal Article https://hdl.handle.net/10568/29422 en Limited Access Cambridge University Press Parasitology;88: 67-84
spellingShingle trypanosoma brucei
parasites
host parasite relations
disease transmission
infection
glossinidae
infectious diseases
parasitology
Barry, J.D.
Emery, J.L.
Parasite development and host responses during the establishment of Trypanosoma brucei infection transmitted by tsetse fly
title Parasite development and host responses during the establishment of Trypanosoma brucei infection transmitted by tsetse fly
title_full Parasite development and host responses during the establishment of Trypanosoma brucei infection transmitted by tsetse fly
title_fullStr Parasite development and host responses during the establishment of Trypanosoma brucei infection transmitted by tsetse fly
title_full_unstemmed Parasite development and host responses during the establishment of Trypanosoma brucei infection transmitted by tsetse fly
title_short Parasite development and host responses during the establishment of Trypanosoma brucei infection transmitted by tsetse fly
title_sort parasite development and host responses during the establishment of trypanosoma brucei infection transmitted by tsetse fly
topic trypanosoma brucei
parasites
host parasite relations
disease transmission
infection
glossinidae
infectious diseases
parasitology
url https://hdl.handle.net/10568/29422
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AT emeryjl parasitedevelopmentandhostresponsesduringtheestablishmentoftrypanosomabruceiinfectiontransmittedbytsetsefly