Characterization of Trypanosoma congolense serodemes in stocks isolated from cattle introduced into a ranch in Kilifi, Kenya
A herd of 20 cattle was introduced on a ranch in Kilifi, Coast Province of Kenya, where they were in contact with Glossina austeni for 6 months. In total, 65 trypanosome isolates were made from these animals. Examination of the isolates revealed that 61 were Trypanosoma congolense and 4 were T. thei...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Cambridge University Press
1987
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/29412 |
| _version_ | 1855531391191613440 |
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| author | Masake, R.A. Nantulya, V.M. Musoke, A.J. Moloo, S.K. Nguli, K. |
| author_browse | Masake, R.A. Moloo, S.K. Musoke, A.J. Nantulya, V.M. Nguli, K. |
| author_facet | Masake, R.A. Nantulya, V.M. Musoke, A.J. Moloo, S.K. Nguli, K. |
| author_sort | Masake, R.A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | A herd of 20 cattle was introduced on a ranch in Kilifi, Coast Province of Kenya, where they were in contact with Glossina austeni for 6 months. In total, 65 trypanosome isolates were made from these animals. Examination of the isolates revealed that 61 were Trypanosoma congolense and 4 were T. theileri. Out of the 61 T. congolense isolates, 55 were successfully passaged and cloned in mice to provide trypanosome populations for further analyses. The stocks and their clones were inoculated into goats on which teneral G. morsitans centralis were later fed in order to provide metacyclics for use in serodeme analysis. Identification of serodemes was carried out by indirect immunofluorescence and neutralization using antimetacyclic hyperimmune sera prepared in mice against metacyclics of cloned trypanosome populations. So far 4 serodemes have been identified in 8 stocks and 7 clones. Each of the 9 stocks contained a mixture of at least 2 of the 4 serodemes identified. Furthermore, stocks isolated sequentially from individual animals contained the same serodemes despite repeated treatment with a curative dose (6 mg/kg body weight) of Berenil between isolations. From the latter finding, it can be inferred that the 4 serodemes were present on the ranch throughout the study period. |
| format | Journal Article |
| id | CGSpace29412 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1987 |
| publishDateRange | 1987 |
| publishDateSort | 1987 |
| publisher | Cambridge University Press |
| publisherStr | Cambridge University Press |
| record_format | dspace |
| spelling | CGSpace294122024-04-25T06:01:17Z Characterization of Trypanosoma congolense serodemes in stocks isolated from cattle introduced into a ranch in Kilifi, Kenya Masake, R.A. Nantulya, V.M. Musoke, A.J. Moloo, S.K. Nguli, K. kilifi district trypanosoma congolense cattle stocks A herd of 20 cattle was introduced on a ranch in Kilifi, Coast Province of Kenya, where they were in contact with Glossina austeni for 6 months. In total, 65 trypanosome isolates were made from these animals. Examination of the isolates revealed that 61 were Trypanosoma congolense and 4 were T. theileri. Out of the 61 T. congolense isolates, 55 were successfully passaged and cloned in mice to provide trypanosome populations for further analyses. The stocks and their clones were inoculated into goats on which teneral G. morsitans centralis were later fed in order to provide metacyclics for use in serodeme analysis. Identification of serodemes was carried out by indirect immunofluorescence and neutralization using antimetacyclic hyperimmune sera prepared in mice against metacyclics of cloned trypanosome populations. So far 4 serodemes have been identified in 8 stocks and 7 clones. Each of the 9 stocks contained a mixture of at least 2 of the 4 serodemes identified. Furthermore, stocks isolated sequentially from individual animals contained the same serodemes despite repeated treatment with a curative dose (6 mg/kg body weight) of Berenil between isolations. From the latter finding, it can be inferred that the 4 serodemes were present on the ranch throughout the study period. 1987-04 2013-06-11T09:23:28Z 2013-06-11T09:23:28Z Journal Article https://hdl.handle.net/10568/29412 en Limited Access Cambridge University Press Masake, R. A., Nantulya, V. M., Musoke, A. J., Moloo, S. K., & Nguli, K. (1987). Characterization ofTrypanosoma congolenseserodemes in stocks isolated from cattle introduced onto a ranch in Kilifi, Kenya. In Parasitology (Vol. 94, Issue 2, pp. 349–357). Cambridge University Press (CUP). https://doi.org/10.1017/s0031182000054007 |
| spellingShingle | kilifi district trypanosoma congolense cattle stocks Masake, R.A. Nantulya, V.M. Musoke, A.J. Moloo, S.K. Nguli, K. Characterization of Trypanosoma congolense serodemes in stocks isolated from cattle introduced into a ranch in Kilifi, Kenya |
| title | Characterization of Trypanosoma congolense serodemes in stocks isolated from cattle introduced into a ranch in Kilifi, Kenya |
| title_full | Characterization of Trypanosoma congolense serodemes in stocks isolated from cattle introduced into a ranch in Kilifi, Kenya |
| title_fullStr | Characterization of Trypanosoma congolense serodemes in stocks isolated from cattle introduced into a ranch in Kilifi, Kenya |
| title_full_unstemmed | Characterization of Trypanosoma congolense serodemes in stocks isolated from cattle introduced into a ranch in Kilifi, Kenya |
| title_short | Characterization of Trypanosoma congolense serodemes in stocks isolated from cattle introduced into a ranch in Kilifi, Kenya |
| title_sort | characterization of trypanosoma congolense serodemes in stocks isolated from cattle introduced into a ranch in kilifi kenya |
| topic | kilifi district trypanosoma congolense cattle stocks |
| url | https://hdl.handle.net/10568/29412 |
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