Monitoring Theileria parva infection in adult Rhipicephalus appendiculatus ticks
A rapid method is described for preparing and staining salivary glands of Rhipicephalus appendiculatus ticks infected with Theileria parva. The technique, involving the use of a modified methyl green pyronin stain, minimizes the risk of losing material and allows examination of stained glands within...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Cambridge University Press
1981
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/29398 |
| _version_ | 1855518185310126080 |
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| author | Irvin, A.D. Boarer, C.D.H. Dobbelaere, D.A.E. Mahan, S.M. Masake, R. Ocama, J.G.R. |
| author_browse | Boarer, C.D.H. Dobbelaere, D.A.E. Irvin, A.D. Mahan, S.M. Masake, R. Ocama, J.G.R. |
| author_facet | Irvin, A.D. Boarer, C.D.H. Dobbelaere, D.A.E. Mahan, S.M. Masake, R. Ocama, J.G.R. |
| author_sort | Irvin, A.D. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | A rapid method is described for preparing and staining salivary glands of Rhipicephalus appendiculatus ticks infected with Theileria parva. The technique, involving the use of a modified methyl green pyronin stain, minimizes the risk of losing material and allows examination of stained glands within minutes of preparation. The technique was applied in a series of studies in which ticks were either infected with T. parva under different conditions, or maturation of parasites in adult ticks was stimulated by different means. When nymphal ticks were fed on the ears of cattle the subsequent infection rate of the adult ticks showed no correlation with the parasitaemia of the cattle at the time of nymphal engorgement. There was no difference in infection rates between adult ticks in which parasite maturation had been stimulated either by incubation at 37 °C or by feeding on rabbits. However, parasite maturation took about 1 day longer in incubated ticks than in rabbit-fed ticks. Female ticks were consistently more highly infected than males, both in terms of the percentage of ticks infected and the mean number of infected acini/tick. Ticks were infected with T. parva by injection of nymphs with parasitaemic bovine blood, but the resultant adult infection was lower than that in ticks which had been infected naturally by feeding on cattle. |
| format | Journal Article |
| id | CGSpace29398 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1981 |
| publishDateRange | 1981 |
| publishDateSort | 1981 |
| publisher | Cambridge University Press |
| publisherStr | Cambridge University Press |
| record_format | dspace |
| spelling | CGSpace293982024-11-15T08:52:14Z Monitoring Theileria parva infection in adult Rhipicephalus appendiculatus ticks Irvin, A.D. Boarer, C.D.H. Dobbelaere, D.A.E. Mahan, S.M. Masake, R. Ocama, J.G.R. rhipicephalus appendiculatus theileria parva infection infectious diseases parasitology A rapid method is described for preparing and staining salivary glands of Rhipicephalus appendiculatus ticks infected with Theileria parva. The technique, involving the use of a modified methyl green pyronin stain, minimizes the risk of losing material and allows examination of stained glands within minutes of preparation. The technique was applied in a series of studies in which ticks were either infected with T. parva under different conditions, or maturation of parasites in adult ticks was stimulated by different means. When nymphal ticks were fed on the ears of cattle the subsequent infection rate of the adult ticks showed no correlation with the parasitaemia of the cattle at the time of nymphal engorgement. There was no difference in infection rates between adult ticks in which parasite maturation had been stimulated either by incubation at 37 °C or by feeding on rabbits. However, parasite maturation took about 1 day longer in incubated ticks than in rabbit-fed ticks. Female ticks were consistently more highly infected than males, both in terms of the percentage of ticks infected and the mean number of infected acini/tick. Ticks were infected with T. parva by injection of nymphs with parasitaemic bovine blood, but the resultant adult infection was lower than that in ticks which had been infected naturally by feeding on cattle. 1981-02 2013-06-11T09:23:26Z 2013-06-11T09:23:26Z Journal Article https://hdl.handle.net/10568/29398 en Limited Access Cambridge University Press Parasitology;82: 137-147 |
| spellingShingle | rhipicephalus appendiculatus theileria parva infection infectious diseases parasitology Irvin, A.D. Boarer, C.D.H. Dobbelaere, D.A.E. Mahan, S.M. Masake, R. Ocama, J.G.R. Monitoring Theileria parva infection in adult Rhipicephalus appendiculatus ticks |
| title | Monitoring Theileria parva infection in adult Rhipicephalus appendiculatus ticks |
| title_full | Monitoring Theileria parva infection in adult Rhipicephalus appendiculatus ticks |
| title_fullStr | Monitoring Theileria parva infection in adult Rhipicephalus appendiculatus ticks |
| title_full_unstemmed | Monitoring Theileria parva infection in adult Rhipicephalus appendiculatus ticks |
| title_short | Monitoring Theileria parva infection in adult Rhipicephalus appendiculatus ticks |
| title_sort | monitoring theileria parva infection in adult rhipicephalus appendiculatus ticks |
| topic | rhipicephalus appendiculatus theileria parva infection infectious diseases parasitology |
| url | https://hdl.handle.net/10568/29398 |
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