Evaluation of cytotoxic lymphocytes and their parasite strain specificity from African buffalo infected with Theileria parva

Summary Theileria parva‐reactive cytotoxic lymphocytes and their precursors were examined in the blood of African buffalo infected with T. parva and uninfected African buffalo. Peripheral blood mononuclear cells (PBM) from eight of 11 infected buffalo were found to have potent cytotoxic activity aft...

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Main Authors: Baldwin, Cynthia L., Malu, M.N., Grootenhuis, J.G.
Format: Journal Article
Language:Inglés
Published: Wiley 1988
Subjects:
Online Access:https://hdl.handle.net/10568/29377
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author Baldwin, Cynthia L.
Malu, M.N.
Grootenhuis, J.G.
author_browse Baldwin, Cynthia L.
Grootenhuis, J.G.
Malu, M.N.
author_facet Baldwin, Cynthia L.
Malu, M.N.
Grootenhuis, J.G.
author_sort Baldwin, Cynthia L.
collection Repository of Agricultural Research Outputs (CGSpace)
description Summary Theileria parva‐reactive cytotoxic lymphocytes and their precursors were examined in the blood of African buffalo infected with T. parva and uninfected African buffalo. Peripheral blood mononuclear cells (PBM) from eight of 11 infected buffalo were found to have potent cytotoxic activity after stimulation with autologous parasitized cells for 6 days in vitro, while PBM from uninfected buffalo or PBM from infected buffalo not stimulated in vitro had no cytotoxic activity. The cytotoxic activity was specific for parasitized cells and genetically restricted since there was no killing on uninfected autologous lymphoblasts and a lower percentage of killing on parasitized allogeneic lymphocytes than on targets of autologous parasitized cells. The cytotoxic cells tested for parasite strain specificity were shown to kill autologous cells transformed with different stocks of both cattle‐derived (T. parva parva) and buffalo‐derived (T. parva lawrencei) parasites.
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spelling CGSpace293772024-05-01T08:16:57Z Evaluation of cytotoxic lymphocytes and their parasite strain specificity from African buffalo infected with Theileria parva Baldwin, Cynthia L. Malu, M.N. Grootenhuis, J.G. lymphocytes parasites buffaloes infection theileria parva animal diseases Summary Theileria parva‐reactive cytotoxic lymphocytes and their precursors were examined in the blood of African buffalo infected with T. parva and uninfected African buffalo. Peripheral blood mononuclear cells (PBM) from eight of 11 infected buffalo were found to have potent cytotoxic activity after stimulation with autologous parasitized cells for 6 days in vitro, while PBM from uninfected buffalo or PBM from infected buffalo not stimulated in vitro had no cytotoxic activity. The cytotoxic activity was specific for parasitized cells and genetically restricted since there was no killing on uninfected autologous lymphoblasts and a lower percentage of killing on parasitized allogeneic lymphocytes than on targets of autologous parasitized cells. The cytotoxic cells tested for parasite strain specificity were shown to kill autologous cells transformed with different stocks of both cattle‐derived (T. parva parva) and buffalo‐derived (T. parva lawrencei) parasites. 1988-07 2013-06-11T09:23:21Z 2013-06-11T09:23:21Z Journal Article https://hdl.handle.net/10568/29377 en Limited Access Wiley Parasite Immunology;10: 393-403
spellingShingle lymphocytes
parasites
buffaloes
infection
theileria parva
animal diseases
Baldwin, Cynthia L.
Malu, M.N.
Grootenhuis, J.G.
Evaluation of cytotoxic lymphocytes and their parasite strain specificity from African buffalo infected with Theileria parva
title Evaluation of cytotoxic lymphocytes and their parasite strain specificity from African buffalo infected with Theileria parva
title_full Evaluation of cytotoxic lymphocytes and their parasite strain specificity from African buffalo infected with Theileria parva
title_fullStr Evaluation of cytotoxic lymphocytes and their parasite strain specificity from African buffalo infected with Theileria parva
title_full_unstemmed Evaluation of cytotoxic lymphocytes and their parasite strain specificity from African buffalo infected with Theileria parva
title_short Evaluation of cytotoxic lymphocytes and their parasite strain specificity from African buffalo infected with Theileria parva
title_sort evaluation of cytotoxic lymphocytes and their parasite strain specificity from african buffalo infected with theileria parva
topic lymphocytes
parasites
buffaloes
infection
theileria parva
animal diseases
url https://hdl.handle.net/10568/29377
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