Differential response of bovine T-cell lines to membrane and soluble antigens of Theileria parva schizont-infected cells
Summary T–cell lines generated from Theileria parva–immunc cattle were used to identify antigens associated with schizont–infected lymphoblastoid cells. Homo–genates prepared from T. parua–infected cells were fractionated by differential centrifugation, and antigenically distinct soluble and membran...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Wiley
1989
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/29376 |
| _version_ | 1855531095093673984 |
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| author | Brown, W.C. Sugimoto, C. Conrad, P.A. Grab, D.J. |
| author_browse | Brown, W.C. Conrad, P.A. Grab, D.J. Sugimoto, C. |
| author_facet | Brown, W.C. Sugimoto, C. Conrad, P.A. Grab, D.J. |
| author_sort | Brown, W.C. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Summary T–cell lines generated from Theileria parva–immunc cattle were used to identify antigens associated with schizont–infected lymphoblastoid cells. Homo–genates prepared from T. parua–infected cells were fractionated by differential centrifugation, and antigenically distinct soluble and membrane–bound antigens were detected by the differential stimulation of cell lines derived from two animals. Activity in the soluble fraction was not attributable to either a mitogen or interleukin 2. Activity in the membrane fraction was associated with schizont membranes as indicated by the presence in this fraction of a parasite protein detected by immunoblot analysis using a schizont–specific monoclonal antibody. Elimination of intracellular schizonts over time, using the anti–theilerial drug, parvaquone, resulted in a concomitant loss of antigenicity in infected cells and in subcellular fractions prepared from drug–treated cells, demonstrating that stimulation of Theileria–sptahc helper and cytotoxic T–cell responses is associated with the presence of the parasite. |
| format | Journal Article |
| id | CGSpace29376 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1989 |
| publishDateRange | 1989 |
| publishDateSort | 1989 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace293762024-05-01T08:18:17Z Differential response of bovine T-cell lines to membrane and soluble antigens of Theileria parva schizont-infected cells Brown, W.C. Sugimoto, C. Conrad, P.A. Grab, D.J. theileria parva metastigmata bovinae infection cattle lymphocytes animal diseases Summary T–cell lines generated from Theileria parva–immunc cattle were used to identify antigens associated with schizont–infected lymphoblastoid cells. Homo–genates prepared from T. parua–infected cells were fractionated by differential centrifugation, and antigenically distinct soluble and membrane–bound antigens were detected by the differential stimulation of cell lines derived from two animals. Activity in the soluble fraction was not attributable to either a mitogen or interleukin 2. Activity in the membrane fraction was associated with schizont membranes as indicated by the presence in this fraction of a parasite protein detected by immunoblot analysis using a schizont–specific monoclonal antibody. Elimination of intracellular schizonts over time, using the anti–theilerial drug, parvaquone, resulted in a concomitant loss of antigenicity in infected cells and in subcellular fractions prepared from drug–treated cells, demonstrating that stimulation of Theileria–sptahc helper and cytotoxic T–cell responses is associated with the presence of the parasite. 1989-11 2013-06-11T09:23:21Z 2013-06-11T09:23:21Z Journal Article https://hdl.handle.net/10568/29376 en Limited Access Wiley Parasite Immunology;11: 567-583 |
| spellingShingle | theileria parva metastigmata bovinae infection cattle lymphocytes animal diseases Brown, W.C. Sugimoto, C. Conrad, P.A. Grab, D.J. Differential response of bovine T-cell lines to membrane and soluble antigens of Theileria parva schizont-infected cells |
| title | Differential response of bovine T-cell lines to membrane and soluble antigens of Theileria parva schizont-infected cells |
| title_full | Differential response of bovine T-cell lines to membrane and soluble antigens of Theileria parva schizont-infected cells |
| title_fullStr | Differential response of bovine T-cell lines to membrane and soluble antigens of Theileria parva schizont-infected cells |
| title_full_unstemmed | Differential response of bovine T-cell lines to membrane and soluble antigens of Theileria parva schizont-infected cells |
| title_short | Differential response of bovine T-cell lines to membrane and soluble antigens of Theileria parva schizont-infected cells |
| title_sort | differential response of bovine t cell lines to membrane and soluble antigens of theileria parva schizont infected cells |
| topic | theileria parva metastigmata bovinae infection cattle lymphocytes animal diseases |
| url | https://hdl.handle.net/10568/29376 |
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