Monoclonal antibodies that distinguish Trypanosoma congolense, Trypanosoma vivax and Trypanosoma brucei
Summary Monoclonal antibodies (MoAbs) were derived against in‐vitro‐propagated procyclic forms of Trypanosoma congolense, T. vivax, T. brucei brucei and T. b. rhodesiense in order to identify antigens for use in immunodiagnosis of African trypanosomiasis. The antibodies have been tested against proc...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Wiley
1987
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| Online Access: | https://hdl.handle.net/10568/29358 |
| _version_ | 1855513865016573952 |
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| author | Nantulya, V.M. Musoke, A.J. Rurangirwa, F.R. Saigar, N. Minja, S.H. |
| author_browse | Minja, S.H. Musoke, A.J. Nantulya, V.M. Rurangirwa, F.R. Saigar, N. |
| author_facet | Nantulya, V.M. Musoke, A.J. Rurangirwa, F.R. Saigar, N. Minja, S.H. |
| author_sort | Nantulya, V.M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Summary Monoclonal antibodies (MoAbs) were derived against in‐vitro‐propagated procyclic forms of Trypanosoma congolense, T. vivax, T. brucei brucei and T. b. rhodesiense in order to identify antigens for use in immunodiagnosis of African trypanosomiasis. The antibodies have been tested against procyclic and bloodstream form trypanosomes of 13 T. congolense, six T. vivax six T. b. brucei, four T. b. rhodesiense, five T. b. gambiense and three T. simiae isolates from different geographical areas by indirect immunofluorescent antibody test (IFAT) and enzyme‐linked immunosorbent assay (ELISA). The MoAbs raised against T. b. brucei reacted with all the brucei group of trypanosomes but not with T. congolense, T. simiae or T. vivax. Likewise, MoAbs against T. congolense reacted with T. congolense and T. simiae but not with any of the other species, while those against T. vivax reacted with T. vivax only. The antigens recognized by these MoAbs were present in lysates of bloodstream trypanosomes as well as midgut (for T. congolense and T. brucei) and epimastigote forms from infected Glossina morsitans centralis. There was no reactivity of the MoAbs with Theileria parva, Anaplasma marginale, Babesia bigemina or Plasmodium falciparum. These antibodies and the antigens they recognize should, therefore, prove useful in the development of assay systems for immunodiagnosis of African trypanosomiasis. |
| format | Journal Article |
| id | CGSpace29358 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1987 |
| publishDateRange | 1987 |
| publishDateSort | 1987 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace293582024-05-01T08:15:13Z Monoclonal antibodies that distinguish Trypanosoma congolense, Trypanosoma vivax and Trypanosoma brucei Nantulya, V.M. Musoke, A.J. Rurangirwa, F.R. Saigar, N. Minja, S.H. monoclonal antibodies trypanosoma vivax trypanosoma congolense trypanosoma brucei animal diseases disease control immunity immunology parasitology Summary Monoclonal antibodies (MoAbs) were derived against in‐vitro‐propagated procyclic forms of Trypanosoma congolense, T. vivax, T. brucei brucei and T. b. rhodesiense in order to identify antigens for use in immunodiagnosis of African trypanosomiasis. The antibodies have been tested against procyclic and bloodstream form trypanosomes of 13 T. congolense, six T. vivax six T. b. brucei, four T. b. rhodesiense, five T. b. gambiense and three T. simiae isolates from different geographical areas by indirect immunofluorescent antibody test (IFAT) and enzyme‐linked immunosorbent assay (ELISA). The MoAbs raised against T. b. brucei reacted with all the brucei group of trypanosomes but not with T. congolense, T. simiae or T. vivax. Likewise, MoAbs against T. congolense reacted with T. congolense and T. simiae but not with any of the other species, while those against T. vivax reacted with T. vivax only. The antigens recognized by these MoAbs were present in lysates of bloodstream trypanosomes as well as midgut (for T. congolense and T. brucei) and epimastigote forms from infected Glossina morsitans centralis. There was no reactivity of the MoAbs with Theileria parva, Anaplasma marginale, Babesia bigemina or Plasmodium falciparum. These antibodies and the antigens they recognize should, therefore, prove useful in the development of assay systems for immunodiagnosis of African trypanosomiasis. 1987-07 2013-06-11T09:23:18Z 2013-06-11T09:23:18Z Journal Article https://hdl.handle.net/10568/29358 en Limited Access Wiley Parasite Immunology;9: 421-431 |
| spellingShingle | monoclonal antibodies trypanosoma vivax trypanosoma congolense trypanosoma brucei animal diseases disease control immunity immunology parasitology Nantulya, V.M. Musoke, A.J. Rurangirwa, F.R. Saigar, N. Minja, S.H. Monoclonal antibodies that distinguish Trypanosoma congolense, Trypanosoma vivax and Trypanosoma brucei |
| title | Monoclonal antibodies that distinguish Trypanosoma congolense, Trypanosoma vivax and Trypanosoma brucei |
| title_full | Monoclonal antibodies that distinguish Trypanosoma congolense, Trypanosoma vivax and Trypanosoma brucei |
| title_fullStr | Monoclonal antibodies that distinguish Trypanosoma congolense, Trypanosoma vivax and Trypanosoma brucei |
| title_full_unstemmed | Monoclonal antibodies that distinguish Trypanosoma congolense, Trypanosoma vivax and Trypanosoma brucei |
| title_short | Monoclonal antibodies that distinguish Trypanosoma congolense, Trypanosoma vivax and Trypanosoma brucei |
| title_sort | monoclonal antibodies that distinguish trypanosoma congolense trypanosoma vivax and trypanosoma brucei |
| topic | monoclonal antibodies trypanosoma vivax trypanosoma congolense trypanosoma brucei animal diseases disease control immunity immunology parasitology |
| url | https://hdl.handle.net/10568/29358 |
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