Purification of African trypanosomes can cause biochemical changes in the parasites
Bloodstream forms of African trypanosomes are routinely purified from blood components by a combination of centrifugation and chromatography on DEAE cellulose at pH 8.0, Here we report that the nonphysiological conditions used for DEAE chromatography of the parasites result in changes in the ATP lev...
| Autores principales: | , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
1987
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/28954 |
| _version_ | 1855543544011292672 |
|---|---|
| author | Lonsdale-Eccles, John D. Grab, D.J. |
| author_browse | Grab, D.J. Lonsdale-Eccles, John D. |
| author_facet | Lonsdale-Eccles, John D. Grab, D.J. |
| author_sort | Lonsdale-Eccles, John D. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Bloodstream forms of African trypanosomes are routinely purified from blood components by a combination of centrifugation and chromatography on DEAE cellulose at pH 8.0, Here we report that the nonphysiological conditions used for DEAE chromatography of the parasites result in changes in the ATP levels of the trypanosomes and an enhanced release from the parasites of proteins such as variable surface glycoprotein, peptidase, and phospholipase. Some of these changes can be reduced by the addition of nucleosides to the elution buffer and, after the elution of the parasites, by immediate readjustment of the external pH to the normal physiological level of blood (pH 7.4). |
| format | Journal Article |
| id | CGSpace28954 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1987 |
| publishDateRange | 1987 |
| publishDateSort | 1987 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace289542024-05-01T08:20:11Z Purification of African trypanosomes can cause biochemical changes in the parasites Lonsdale-Eccles, John D. Grab, D.J. trypanosoma purification parasites disease transmission animal diseases Bloodstream forms of African trypanosomes are routinely purified from blood components by a combination of centrifugation and chromatography on DEAE cellulose at pH 8.0, Here we report that the nonphysiological conditions used for DEAE chromatography of the parasites result in changes in the ATP levels of the trypanosomes and an enhanced release from the parasites of proteins such as variable surface glycoprotein, peptidase, and phospholipase. Some of these changes can be reduced by the addition of nucleosides to the elution buffer and, after the elution of the parasites, by immediate readjustment of the external pH to the normal physiological level of blood (pH 7.4). 1987-11 2013-05-06T07:01:50Z 2013-05-06T07:01:50Z Journal Article https://hdl.handle.net/10568/28954 en Limited Access Wiley Journal of Protozoology;34: 405-408 |
| spellingShingle | trypanosoma purification parasites disease transmission animal diseases Lonsdale-Eccles, John D. Grab, D.J. Purification of African trypanosomes can cause biochemical changes in the parasites |
| title | Purification of African trypanosomes can cause biochemical changes in the parasites |
| title_full | Purification of African trypanosomes can cause biochemical changes in the parasites |
| title_fullStr | Purification of African trypanosomes can cause biochemical changes in the parasites |
| title_full_unstemmed | Purification of African trypanosomes can cause biochemical changes in the parasites |
| title_short | Purification of African trypanosomes can cause biochemical changes in the parasites |
| title_sort | purification of african trypanosomes can cause biochemical changes in the parasites |
| topic | trypanosoma purification parasites disease transmission animal diseases |
| url | https://hdl.handle.net/10568/28954 |
| work_keys_str_mv | AT lonsdaleecclesjohnd purificationofafricantrypanosomescancausebiochemicalchangesintheparasites AT grabdj purificationofafricantrypanosomescancausebiochemicalchangesintheparasites |